EP-4741510-A1 - METHOD FOR PRODUCING HIGH-PURITY ARGININE

Abstract

The present application relates to a method for producing arginine, wherein a bleaching step is not performed, and an anion exchange step is separated and performed simultaneously with an arginine production step, thereby improving the yield of arginine and significantly reducing the time required for production.

Inventors

• HAN, HYEONG SEOK
• KIM, JOONGHO

Assignees

• CJ Cheiljedang Corporation

Dates

Publication Date

20260513

Application Date

20240904

Claims (11)

1. A method for producing arginine comprising: (a) passing a fermentation broth containing arginine through a cation exchange resin to obtain an eluate; (b) concentrating and crystallizing the eluate obtained in step (a); (c) passing the mother liquor obtained in step (b) through an anion exchange resin to obtain an eluate; and (d) reusing the eluate obtained in step (c) in step (a), (b), or both steps (a) and (b), wherein steps (a) through (d) are repetitively performed.
2. The method according to Claim 1, wherein the fermentation broth containing arginine is a fermentation product obtained by culturing a microorganism producing arginine, or a fermentation product from which the cells are removed.
3. The method according to Claim 1, wherein step (a) further comprises a step of concentrating the eluate passed through the cation exchange resin to a concentration of 200 g/l or less,
4. The method according to Claim 1, wherein step (b) is to concentrate arginine to a concentration of 200 g/l or more of arginine concentration.
5. The method according to Claim 1, wherein the concentrating in step (b) is performed at 30 to 60 °C.
6. The method according to Claim 1, wherein step (b) further comprises a step of cooling the concentrate to 35 °C or less.
7. The method according to Claim 1, wherein step (b) further comprises a step of drying the obtained arginine.
8. The method according to Claim 1, characterized in that step (c) is performed concurrently with steps (a) and (b).
9. The method according to any one of Claim 1 to Claim 8, characterized in that a decolorization process is not performed'
10. The method according to any one of Claims 1 to 8, wherein the time required for producing arginine is reduced by 20% or more, compared with a method for producing arginine in which the anion exchange process is not performed separately.
11. The method according to any one of Claims 1 to 8, wherein the method for producing arginine is a method for producing arginine crystals or arginine powder.

Description

[TECHNICAL FIELD] CROSS-REFERENCE TO RELATED APPLICATION(S) This application claims the benefit of priority based on Korean Patent Application No. 10-2023-0117455 filed on September 5, 2023, and all contents disclosed in the document of the corresponding Korean Patent Application are incorporated herein by reference. The present application relates to a method for producing high-purity arginine. [BACKGROUND ART] The present invention relates to a method for producing high-purity arginine used in animal feed additives, etc., from arginine fermentation broth. The conventional method for producing arginine includes the steps of separating bacterial cells from the arginine fermentation broth, cation exchange process, decolorization process, anion exchange process, followed by crystallization and separation. Among these, the decolorization process is a step in which activated carbon is added to absorb and separate organic impurities or colored ionic substances contained in the arginine process liquid, thereby improving the purity of the arginine solution and the transmittance of arginine. However, a demand for high transmittance in arginine products used for animal feed additives has been decreasing recently, and environmental issues concerning the disposal of used activated carbon and wastewater from the decolorization process are becoming increasingly prominent. [PRIOR ART] (Patent document 1) US 2003-0124686 A1 [DISCLOSURE] [TECHNICAL PROBLEM] An object of the present application is to provide a method for producing arginine. [TECHNICAL SOLUTION] The present application relates to a method for producing arginine in which a decolorization process is not performed, and an anion exchange process is separated and carried out concurrently with the arginine production process, thereby the yield of arginine is enhanced and the time required for arginine production is drastically reduced. An aspect provides a method for producing arginine. The arginine may be arginine crystals or arginine powder. Definition of Terms In the present application, the term "fermentation product" may mean a resultant product of enzymatic or metabolic decomposition of organic material utilizing a microorganism. For example, the fermentation product may include a culture product itself obtained by culturing the microorganism in a culture medium, the culture product from which cells (the microorganism, or a part of the microorganism) are removed, or a concentrated product, a dry product, or a lyophilized product of the culture product from which the bacterial cells are removed. Furthermore, in this case, the term "fermentation broth" may include the whole fermentation product containing arginine produced from the microorganism, or may be one from which impurities are removed from the fermentation product. In the present application, the term "eluate" may mean the liquid having passed through a cation exchange resin and/or an anion exchange resin. In the present application, the term "process liquid" may refer collectively to any liquid that is introduced into each step for producing arginine (e.g., arginine crystals) and/or any liquid obtained after each processing step. For example, the process liquid may include a fermentation broth, eluate, concentrate, filtrate containing arginine, and the like. In this application, the term "mother liquor" may refer to the liquid remaining after separation of arginine (e.g., arginine crystals) by performing a concentration-crystallization process on a process liquid containing arginine Fermentation broth containing arginine An aspect may provide a method for producing arginine crystals from the fermentation broth containing arginine. The fermentation broth containing arginine may be obtained by culturing a microorganism producing arginine. The microorganism producing arginine includes both wild-type microorganisms or microorganisms having natural or artificial genetic modification. Such a microorganism may contain a genetic modification of a target protein for arginine production, and may be a microorganism in which a specific mechanism is weakened or enhanced due to any cause such as insertion of a foreign gene, or strengthened or inactivated activity of intrinsic gene. The microorganism producing arginine according to the present application may be a microorganism naturally possessing arginine production capability, or a microorganism on which arginine production capability is conferred to a microorganism lacking arginine production capability, but is not limited thereto. Specifically, the microorganism producing arginine or the microorganism having arginine production capability in the present application, may be a microorganism in which a part of the genes within the arginine biosynthetic pathway is enhanced or weakened, or a part of the genes within the arginine degradation pathway is enhanced or weakened. In the present application, the terms "enhanced" or "increased" arginine production capabilit