Search

EP-4741804-A1 - DEVICE AND METHOD FOR IMAGE ACQUISITION

EP4741804A1EP 4741804 A1EP4741804 A1EP 4741804A1EP-4741804-A1

Abstract

A first aspect of the present disclosure is related to a device for image acquisition, configured to: - select a plurality of excitations for exciting a sample, - adjust in a first step: -- a configuration of one of the selected excitations or; -- a configuration of a global excitation , wherein the global excitation is based on one or more of the selected excitations; - adjust in a second step and based on the configuration adjusted in the first step: -- a configuration of one or more of the non-adjusted, selected excitations; and/or -- a configuration of the global excitation, respectively; - providing the adjusted configurations for performing an excitation of the sample.

Inventors

  • WELTI, STEFAN
  • REYMANN, JUERGEN

Assignees

  • Leica Microsystems CMS GmbH

Dates

Publication Date
20260513
Application Date
20241106

Claims (15)

  1. Device for image acquisition, configured to: - select a plurality of excitations (112, 114, 116, 118) for exciting a sample, - adjust in a first step: -- a configuration (116a, 116b) of one of the selected excitations (106) or; -- a configuration (122a, 122b) of a global excitation (122), wherein the global excitation is based on one or more of the selected excitations; - adjust in a second step and based on the configuration adjusted in the first step: -- a configuration (112a, 112b; 114a, 114b; 118a, 118b) of one or more of the non-adjusted, selected excitations; and/or -- a configuration (122a, 122b) of the global excitation, respectively; - providing the adjusted configurations (112a, 114a, 116a, 118a, 112b, 114b, 116b, 118b) for performing an excitation of the sample.
  2. The device according to the preceding claim, configured to: - obtain selection information from a user interface (100a, 100b), and wherein the selection of the excitations is based on the obtained selection information; - obtain adjustment information from the user interface, and wherein the adjustment of the configuration of the excitation in the first step is based on the obtained adjustment information.
  3. The device according to one of the preceding claims, wherein a configuration of an excitation can comprise one or more of: - frequency-dependent information; - time-dependent information.
  4. The device according to one of the preceding claims, wherein an adjustment of the configuration of one or more of the excitations (112, 114, 116, 118) comprises an adjustment of an intensity of the excitation.
  5. The device according to one of the preceding claims, wherein the global excitation (122) is based on the selected excitation with a predefined parameter, in particular intensity (116), that is largest relative to the parameter of the other selected excitations, or based on a compounded excitation based on two or more of the selected excitations.
  6. The device according to one of the preceding claims, wherein the adjustment of a configuration (112a, 112b; 114a, 114b; 118a, 118b) of one or more of the non-adjusted excitations is based on a linear scaling of a configuration parameter of the one or more non-adjusted excitations relative to a configuration parameter of the adjusted excitation (116a, 116b).
  7. The device according to one of the preceding claims, wherein the adjustment of a configuration of one or more of the excitations (112, 114, 116, 118) is based on a pre-defined requirement for unmixing excited emissions of an excited sample.
  8. The device according to one of the preceding claims, wherein the adjustment of a configuration of one or more of the excitations (112, 114, 116, 118) is based on a characteristic of an emission-signal, in particular a dispersion, excited by a configured excitation (112, 114, 116, 118).
  9. The device according to one of the preceding claims, wherein the adjustment of a configuration of one or more of the excitations (112, 114, 116, 118, 122) is based on a pre-defined intensity of a staining of a fluorophore within an image.
  10. The device according to one of the preceding claims, wherein the adjustment of a configuration of one or more of the excitations (112, 114, 116, 118, 122) is based on pre-defined depth within a stained image.
  11. The device according to one of the preceding claims, wherein the adjustment of a configuration of one or more of the excitations (112, 114, 116, 118, 122) is based on a pre-defined brightness of an image of the sample.
  12. The device according to one of the preceding claims, wherein the adjustment of a configuration of one or more of the excitations (112, 114, 116, 118, 122) is based on a pre-defined SNR, in particular a degree of autofluorescence, of an image of the sample.
  13. The device according to one of the preceding claims, configured to: - display the configurations of the adjusted excitations; - display a range in which an adjustment of a configuration of one or more of the excitations (112, 114, 116, 118, 122) satisfies a pre-defined requirement, in particular an unmixing requirement.
  14. The device according to one of the preceding claims, configured to: - receive information about a further adjustment of a respective configuration of one or more or the selected excitations.
  15. A computer-implemented method for image acquisition, comprising the steps: - selecting a plurality of excitations (112, 114, 116, 118) for exciting a sample, - adjusting a configuration of one of the selected excitations or of a global excitation (122), wherein the global excitation is based on one or more of the selected excitations; - adjust a configuration of one or more of the non-adjusted selected excitations and/or of the global excitation, based on the adjusted excitation; - providing the adjusted configurations (112a, 114a, 116a, 118a, 112b, 114b, 116b, 118b) for performing an excitation of the sample.

Description

Technical Field This disclosure is related to devices for image acquisition and methods therefor. Background In fluorescence imaging, various problems can occur that make it necessary to adjust the configurations for one or more excitation channels. These can be, for example, fluctuations in the strength of individual colorations or the coloration as a whole, as well as differences in the depth of penetration due to the respective new selection of the recording area. For subsequent image processing steps (e.g. unmixing), further requirements may have to be considered. Overall, the adjustment process is cumbersome and timeconsuming. Improvements are desired. Summary An object of the present disclosure is to improve fluorescence imaging. This object is solved by the disclosed embodiments, which are defined in particular by the subject matter of the independent claims. The dependent claims provide information for further embodiments. Various aspects and embodiments of these aspects are also disclosed in the summary and description below, which provide additional features and advantages. A first aspect of the present disclosure is related to a device for image acquisition, configured to: select a plurality of excitations for exciting a sample,adjust in a first step:- a configuration of one of the selected excitations or;-- a configuration of a global excitation , wherein the global excitation is based on one or more of the selected excitations;- adjust in a second step and based on the configuration adjusted in the first step:-- a configuration of one or more of the non-adjusted, selected excitations; and/or-- a configuration of the global excitation, respectively;- providing the adjusted configurations for performing an excitation of the sample. A device for image acquisition can be an imaging device, e.g., a microscope, an endoscope, an exoscope, or it can be a device that is configured to exchange information with an imaging device. If the device exchanges information with an imaging device, the device can be a network device and in particular be in communication with a plurality of imaging devices. The term "excitation" refers to a process where light at a specific wavelength energizes a fluorophore in a sample, causing the fluorophore to absorb energy and enter an excited state. After this, the fluorophore quickly returns to its ground state, releasing energy as fluorescence emission, in particular at a longer wavelength. The fluorescence emission can then be captured to create an image. Excitation and emission wavelengths may be selected using filters to ensure only the desired light is used and detected. In other words, an excitation can relate to one or more light configurations to excite a target fluorophore. Selecting a plurality of excitations in the context of this disclosure means that a plurality of light configurations are selected. Each light configuration can comprise one or more parameters such as frequency, intensity, and/or transient of an exciting light. Additionally or alternatively, an excitation can be indirectly defined by one or more parameters of the florescence emission, such as frequency, intensity, and/or transient behavior of the respective fluorescence emission. Another indirect emission-related parameter which can be additionally or alternatively comprised by an excitation can be related to a crosstalk, e.g. an extend of an excitation of a fluorophore, which is not targeted by the excitation. Adjusting a configuration of an excitation in the context of this disclosure means that at least one parameter of an excitation is adapted, e.g. set to an intended value. An adjustment, in particular an adjustment of an excitation in the first step, can be performed manually and/or automatically. An adjustment of a (leading) excitation in a first step follows, in a second step, an adjustment of other selected excitations, in particular all other selected excitations. Hence, based on an intended value adjusted for a parameter of an excitation in the first step, other values for parameters of other excitations are adjusted. Hence, a problem of adjusting a plurality of excitation can be reduced to the problem of adjusting only one excitation and wherein the other excitations are following the adjustment of the first excitation. This may simplify an automatic and/or manual adjustment process of a plurality of excitations intended to be used for exciting a sample stained with a plurality of fluorophores. The adjusted configurations for the different excitation can be provided to the optical unit of the imaging device. In case the device is an imaging device, the information can be provided internally. In case, the device is not an imaging system but in communication with an imaging system, the information can be provided such that an intended imaging system can use this information. A provision of information, e.g. configuration information about an excitation, in the sense of this disclosure ca