JP-2022512979-A5 -

Dates

Publication Date

20221215

Application Date

20191107

Description

In some embodiments, the algae are cultured under dark conditions. These dark conditions preferably have an illuminance of less than 1000 lux, less than 750 lux, less than 500 lux, less than 400 lux, less than 300 lux, less than 200 lux, or less than 100 lux. In some embodiments, algae cultured under dark conditions have at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, or at least 80% less chlorophyll production compared to algae cultured under non-dark conditions. In some embodiments, algae grown under dark conditions are supplemented with one or more nutrients. In some embodiments, algae grown under dark conditions are grown in the presence of a reducing carbon source such as acetate, glucose, sucrose, fructose, glycerol, or other types of sugars (e.g., dextrose, maltose, galactose, sucrose, ribose, etc.). In some embodiments, algae grown under dark conditions are grown in the presence of iron, or if not, are supplemented with iron. Example 3: Preparation of a PPIX-rich "meat-free" hamburger A composition of a meat-like product made from plant-based materials and PPIX-enriched algae was prepared using a PPIX-enriched sample. To create a PPIX-rich hamburger, the materials were mixed in the following proportions and formed into a disc-shaped plant-based hamburger using algae: The proportions were 20% or about 20% wheat-derived textured protein, 20% or about 20% refined coconut oil, 3% or about 3% sunflower oil, 2% or about 2% potato starch, 0.5% or about 0.5% konjac gum, 0.5% or about 0.5% xanthan gum, 45% or about 45 % water, and 4-9% or about 4-9% seasonings (including yeast extract, garlic powder, onion powder, and salt), and PPIX-enriched ("red") algae. Figure 3 shows hamburgers made using 0.01 g, 0.1 g, 1.0 g, and 5.0 g of PPIX-enriched algae.