JP-2022532802-A5 -

Dates

Publication Date

20230525

Application Date

20200522

Description

[Invention 1001]A transfer plasmid comprising an expression cassette containing a coding polynucleotide encoding an isoform or functional variant of T cell immunomodulator 1 (TCIRG1) and a promoter, wherein the polynucleotide is operably ligated to the promoter, and the transfer plasmid comprises an RNA-OUT repressor and a CMV IE promoter.[Invention 1002]The transfer plasmid of the present invention 1001, wherein the RNA-OUT repressor shares at least 95% identity or at least 99% identity with SEQ ID NO: 32.[Invention 1003]A transfer plasmid according to Invention 1001 or Invention 1002, wherein the CMV IE promoter shares at least 95% identity or at least 99% identity with SEQ ID NO: 33.[Invention 1004]A transfer plasmid according to any one of invention 1001 to 1003, comprising a pCCL skeleton.[Invention 1005]The transfer plasmid of the present invention 1004, wherein the pCCL backbone contains the RNA-OUT repressor.[Invention 1006]A transfer plasmid of the present invention 1005, sharing at least 95% or 100% identity with sequence number 39.[Invention 1007]A transfer plasmid according to any one of the present invention 1001 to 1007, wherein the promoter is an EFS promoter.[Invention 1008]The transfer plasmid of the present invention 1007, wherein the EFS promoter shares at least 95% identity with SEQ ID NO: 2.[Invention 1009]The transfer plasmid of the present invention 1008, wherein the EFS promoter is sequence number 2.[Invention 1010]A transfer plasmid according to any one of the present invention 1001 to 1009, wherein the coding polynucleotide shares at least 95% identity with SEQ ID NO: 3.[Invention 1011]The transfer plasmid of the present invention 1010, wherein the coding polynucleotide shares at least 99% identity with SEQ ID NO: 3.[Invention 1012]The transfer plasmid of the present invention 1011, wherein the coding polynucleotide is sequence number 3.[Invention 1013]The expression cassette comprises a transfer plasmid according to any one of the inventions 1001 to 1012, wherein the expression cassette contains a woodchuck hepatitis virus (WHP) post-transcriptional regulatory element (WPRE).[Invention 1014]The transfer plasmid of the present invention 1013, wherein the aforementioned WPRE is sequence number 4.[Invention 1015]A transfer plasmid according to any one of the present invention 1001 to 1014, wherein the expression cassette shares at least 95% identity with SEQ ID NO: 1.[Invention 1016]A transfer plasmid according to any one of the present invention 1001 to 1015, wherein the expression cassette is adjacent to a 5' long terminal repeat sequence (LTR) and a 3' LTR.[Invention 1017]A transfer plasmid according to the present invention 1016, wherein the 5'LTR is sequence number 34 and/or the 3'LTR is sequence number 28.[Invention 1018]A transfer plasmid according to any one of the present invention 1001 to 1017, wherein the expression cassette shares at least 95% identity with SEQ ID NO: 1.[Invention 1019]A transfer plasmid according to any of the present invention 1001 to 1017, wherein the expression cassette is sequence number 1.[Invention 1020]Lentiviral particles produced by transfecting a host cell with any of the transfer plasmids of the present invention 1001 to 1020.[Invention 1021]An expression cassette comprising a coding polynucleotide encoding an isoform or functional variant of T cell immunomodulatory factor 1 (TCIRG1) and an EFS promoter, wherein the polynucleotide is operably ligated to the EFS promoter.[Invention 1022]An expression cassette of the present invention 1001, wherein the coding polynucleotide shares at least 95% identity with SEQ ID NO: 3.[Invention 1023]The expression cassette of the present invention 1002, wherein the coding polynucleotide shares at least 99% identity with SEQ ID NO: 3.[Invention 1024]An expression cassette of the present invention 1003, wherein the coding polynucleotide is sequence number 3.[Invention 1025]An expression cassette according to any of the present invention 1001 to 1004, wherein the EFS promoter shares at least 95% identity with SEQ ID NO: 2.[Invention 1026]An expression cassette of the present invention 1025, wherein the EFS promoter is sequence number 2.[Invention 1027]An expression cassette according to any of the inventions 1021 to 1026, comprising a woodchuck hepatitis virus (WHP) post-transcriptional regulatory element (WPRE).[Invention 1028]An expression cassette for the present invention 1027, wherein the aforementioned WPRE is sequence number 4.[Invention 1029]An expression cassette of any of the present invention 1021 to 1028, sharing at least 95% identity with Sequence ID No. 1.[Invention 1030]The expression cassette for the invention 1029, which is sequence number 1 of sequence number 1.[Invention 1031]Recombinant lentiviral genome, in the order of 5' to 3',(a) Lentivirus 5' long-term repeat sequence (LTR),(b) An expression cassette of any of the inventions 1021 to 1030,(c) Lentivirus 3'LTR andA recombinant lentiviral g