JP-2025539666-A5 -

Dates

Publication Date

20260508

Application Date

20230426

Description

Equivalents: Each and all patent, patent application, and publication disclosures referenced herein are incorporated herein by reference in their entirety. While the present invention has been disclosed by reference to certain embodiments, it will be apparent that other embodiments and variations of the present invention can be invented by those skilled in the art without departing from the true spirit and scope of the invention. The appended claims are intended to be construed to include all such embodiments and equivalent variations. In a particular embodiment, for example, the following items are provided: (Item 1) A method for regulating the production of low molecular weight halogenated organic compounds using peroxidase enzymes, (i) supplying low molecular weight organic compounds (e.g., acetylacetone), (ii) Contacting the low molecular weight organic compound with a peroxidase (e.g., VHPO) to form a reaction mixture under conditions sufficient to produce a low molecular weight halogenated organic compound; (iii) Evaluating the produced low molecular weight halogenated organic compound and thereby regulating the production of the low molecular weight halogenated organic compound. (Item 2) The method according to item 1, wherein the peroxidase is a haloperoxidase. (Item 3) The method according to item 2, wherein the haloperoxidase is vanadium haloperoxidase (VHPO). (Item 4) The method according to item 3, wherein the VHPO is vanadium chloroperoxidase (VCCO), vanadium bromoperoxidase (VBPO), or vanadium iodoperoxidase (VIPO). (Item 5) The method described in any one of items 3 to 4, wherein the VHPO is a VBPO. (Item 6) The peroxidase is an algal haloperoxidase (e.g., derived from algae), a fungal haloperoxidase (e.g., derived from a fungal species), or a cyanobacterial haloperoxidase (e.g., derived from cyanobacteria), as described in item 1. (Item 7) The method according to item 1, wherein the peroxidase is a fungal haloperoxidase (for example, derived from a fungal species). (Item 8) The peroxidase is derived from Curvularia inaqualis, Halomicronema hongdechloris, Moorea bouillonii, Trichodesmium erythraeum, Aphanocapsa. montana, Lyngbya confervoides, Synechococcus The method according to item 1, derived from organisms selected from sp. PCC7335 and Corallina officinalis. (Item 9) The peroxidase is produced within a host cell microorganism, as described in item 1. (Item 10) The method according to item 9, wherein the host cell microorganism is selected from Pichia pastoris, Aspergillus niger, Saccharomyces cerevisiae, or Escherichia coli. (Item 11) The method according to any one of items 9 to 10, wherein the expression of the peroxidase produced in the host cell microorganism is, for example, about 1.5 times, 2 times, 3 times, 4 times, 5 times, 6 times, 7 times, 8 times, or 10 times higher than the peroxidase produced in the natural host. (Item 12) The method according to item 1, wherein the amino acid sequence of the peroxidase is selected from the amino acid sequences listed in Table 1. (Item 13) The method according to item 1, wherein the peroxidase has at least 75% sequence identity with respect to the peroxidase sequences listed in Table 1 (for example, at least 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99%, or 99.5% sequence identity). (Item 14) The method according to item 1, wherein the peroxidase is a sequence selected from any one of sequence numbers 1 to 50. (Item 15) The method according to item 1, wherein the amino acid sequence of the peroxidase has 1, 2, 3, 4, 5, or 6 amino acid substitutions to an amino acid sequence selected from any one of sequence numbers 1 to 50. (Item 16) The low molecular weight organic compound is, for example, the method described herein, having the structure of formula (Y). (Item 17) The method according to item 1, wherein the low molecular weight organic compound contains 1, 2, 3, 4, 5, 6, 7, or 8 carbon atoms. (Item 18) The method according to item 1, wherein the low molecular weight organic compound comprises a ketone or an aldehyde. (Item 19) The low molecular weight halogenated organic compound is, for example, the method described herein, having the structure of formula (Z). (Item 20) The low molecular weight halogenated organic compound is chlorinated, brominated, iodized, chlorinated and iodized, chlorinated and brominated, brominated and iodized, or chlorinated, brominated and iodized, as described in item 1. (Item 21) The method according to item 1, wherein the low molecular weight halogenated organic compound is brominated. (Item 22) The low molecular weight halogenated organic compound contains one, two, or three halogen atoms, as described in item 1. (Item 23) The low molecular weight halogenated organic compound is the method according to item 1, wherein the low molecular weight halogenated organic compound contains one, two, or three bromine atoms. (Item 24) The method according to item 1, wherein the low molecular weight halog