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JP-2026074677-A - Collection system, collection method, and collection program

JP2026074677AJP 2026074677 AJP2026074677 AJP 2026074677AJP-2026074677-A

Abstract

[Problem] To quickly retrieve the target object on the sample plate. [Solution] A recovery system according to one embodiment comprises: a set stand that supports a sample plate on which sample sections are placed; a container holder that holds a recovery container for recovering target objects from the sample sections on the sample plate; a main blade; a first moving structure that supports the main blade so that the tip of the main blade is movable along the surface of the sample plate supported by the set stand; at least one first actuator that generates power to move the main blade via the first moving structure; at least one sub-blade; at least one second moving structure that supports at least one sub-blade so that it is movable relative to the tip of the main blade; and at least one second actuator that generates power to move at least one sub-blade via the at least one second moving structure. [Selection Diagram] Figure 2

Inventors

  • 山本 浩一
  • 森 邦義

Assignees

  • 川崎重工業株式会社

Dates

Publication Date
20260507
Application Date
20241021

Claims (13)

  1. A set stand that supports the sample plate on which the sample section is placed, A container holder for holding a collection container for collecting target objects from the sample sections on the sample plate, The main blade and A first movable structure supports the main blade so that the tip of the main blade can move along the surface of the sample plate supported on the set stand, At least one first actuator that generates power to move the main blade via the first moving structure, At least one secondary blade, A second movable structure that supports the at least one sub-blade so as to be movable with respect to the tip of the main blade, A recovery system comprising: at least one second actuator that generates power to move the at least one sub-blade via the at least one second moving structure.
  2. The recovery system according to claim 1, wherein the second moving structure is configured to support the secondary blade so as to be movable parallel to the rake surface of the main blade.
  3. The recovery system according to claim 1 or 2, wherein the at least one sub-blade includes a first sub-blade facing the rake face of the main blade and a second sub-blade facing the relief face of the main blade.
  4. The recovery system according to claim 1 or 2, wherein the set stand is configured to support the sample plate such that the surface on which the sample section is placed faces upward.
  5. It is further equipped with processing circuits, The aforementioned processing circuit is Controlling at least one of the first actuators to move the tip of the main blade along the surface of the sample plate to perform a dissection process to remove the target object from the sample section on the sample plate, and Controlling at least one second actuator to move relative to the tip of the main blade to perform a scraping process to scrape off the target object adhering to the tip of the main blade into the collection container, A recovery system according to claim 1 or 2, configured to perform the following:
  6. The recovery system according to claim 5, wherein the processing circuit is configured to perform a positioning process after the dicing process and before the scraping process, by controlling at least one first actuator to position the tip of the main blade so that it overlaps with the recovery container in a top view.
  7. The setting stand supports the sample plate such that the surface of the sample plate is perpendicular to or inclined with respect to the horizontal plane. The recovery system according to claim 5, wherein the dissection process includes controlling the at least one first actuator so that the tip of the main blade moves upward along the surface of the sample plate via the first moving structure.
  8. The recovery system according to claim 5, wherein the positioning process includes controlling the at least one first actuator to pass the tip of the main blade through the opening of the recovery container and position the tip of the main blade within the recovery container.
  9. The first movable structure includes a frame body on which the main blade is supported, and a retainer that detachably holds the frame body, The recovery system according to claim 1 or 2, wherein the at least one second moving structure is supported by the frame body.
  10. The recovery system according to claim 9, wherein at least one second actuator is supported by the retainer without passing through the frame body.
  11. The first movable structure includes a frame body and a blade support structure that supports the main blade relative to the frame body, and the first movable structure is configured to move the main blade together with the frame body. The recovery system according to claim 1 or 2, wherein the blade support structure includes a biasing member that biases the main blade in a direction from the base end to the tip of the main blade.
  12. A system comprising at least one first actuator for generating power to move a main blade and at least one second actuator for generating power to move at least one sub-blade relative to the tip of the main blade, wherein a recovery method for recovering a target object from a sample section on a sample plate into a recovery container, Controlling at least one of the first actuators to move the tip of the main blade along the surface of the sample plate to scrape off the target object from the sample section on the sample plate, and Controlling at least one second actuator to move at least one sub-blade relative to the tip of the main blade to scrape off the target object adhering to the tip of the main blade into the collection container, A collection method that includes this.
  13. A recovery program that causes at least one processor to execute the recovery method described in claim 12.

Description

This disclosure relates to a recovery system, recovery method, and recovery program. Methods for recovering target objects from sample plates containing them have been known for some time. For example, Patent Document 1 discloses a micromanipulation method in which a target object is recovered by suction after being suspended by dropping a release solution onto the sample plate. Other methods for recovering target objects from sample plates include laser microdissection, which involves cutting the surrounding tissue of the target object on the sample plate with a laser under microscopic observation to recover the target object, and macrodissection, which involves scraping the target object from the sample plate using a scalpel or similar tool under visual observation. Patent No. 4578814 Figure 1 is a schematic diagram of the recovery system according to the embodiment.Figure 2 is a perspective view of a dissection apparatus.Figure 3 is a perspective view of the set stand.Figure 4 is an enlarged perspective view of the dicing tool and the holder that holds the dicing tool.Figure 5 is an enlarged side view showing the positional relationship between the dicing tool and the solenoid actuator.Figure 6 is a view of the main blade and the blade support structure that supports the main blade, as seen from a direction perpendicular to the main blade.Figure 7 is a schematic side view illustrating the positional relationship between the tips of the main blade, the first secondary blade, and the second secondary blade.Figure 8 is a block diagram showing the electrical system of the recovery system.Figure 9 shows the flow of the collection process by the collection system.Figure 10 shows an example of a settings screen displayed on the screen.Figure 11 is a side view illustrating the positional relationship between the sample plate and the main blade during the dissection process.Figure 12 is a partial side cross-sectional view illustrating the positional relationship between the recovery container, the main blade, the first sub-blade, and the second sub-blade. The embodiments will be described below with reference to the drawings. (Background leading to the development of the system) Conventionally, so-called macro-dissection, which involves scraping off the target object from a sample plate containing the target object using a scalpel or similar tool, has been performed manually by an operator, which is time-consuming and labor-intensive. Furthermore, there was room for improvement in conventional macro-dissection from the standpoint of homogeneity of the sample scraped off from the sample plate by an operator. The recovery system according to this embodiment was constructed from this perspective, and realizes the automation of some or all of the macro-dissection process. (Overall system configuration) Figure 1 is a schematic diagram of the recovery system 1 according to this embodiment. Figure 2 is a perspective view of the dissection apparatus 3. Note that the housing 10, which will be described later, is omitted in Figure 2. The recovery system 1 is a system for scraping and recovering target objects to be recovered from a sample plate 100, which is a substrate such as a glass slide on which sample sections are placed. The recovery system 1 comprises a dissection apparatus 3 and an information processing apparatus 4. The housing 10 includes a workbench 11 on which the dissection device 3 is placed, a fixed cover 12 fixed to the workbench 11, and a movable cover 13 that is movable relative to the fixed cover 12. The movable cover 13 is movable between an open position and a closed position relative to the fixed cover 12. As shown in Figure 1, when the movable cover 13 is in the open position, the operator M can perform various tasks inside the housing 10. These tasks include, for example, replacing the dissection tool 70 and the sample plate holder 31, as described later. When the movable cover 13 is in the closed position, the fixed cover 12 and the movable cover 13 prevent the operator M from accessing the inside of the housing 10 from the outside. In the following description, the left-right direction is referred to as the X direction, the front-back direction as the Y direction, and the up-down direction as the Z direction, from the perspective of the worker M working inside the enclosure 10. The X and Y directions are perpendicular to each other on the horizontal plane. The Z direction is the vertical direction. Furthermore, the +X direction may be referred to as the right side, the -X direction as the left side, the +Y direction as the back side, the -Y direction as the front side, the +Z direction as the top side, and the -Z direction as the bottom side. However, these directions are merely for explanatory purposes and do not limit the following embodiments in any way. The information processing device 4 includes an input device 21, a display device 22, and a main control device 23. The input device 21 is a