JP-2026074826-A - Sample measuring device

JP2026074826AJP 2026074826 AJP2026074826 AJP 2026074826AJP-2026074826-A

Abstract

[Problem] To provide a specimen measurement device that can streamline specimen testing operations, including malaria infection testing. [Solution] The specimen measuring device 1, which measures a specimen taken from a subject, includes a measuring unit 10 that prepares a measurement sample from the specimen and reagents and detects an optical signal corresponding to at least cells in the measurement sample, and an analysis unit 30 that analyzes cells in accordance with the measurement of the specimen by the measuring unit 10. The measuring unit 10 is capable of selectively performing a plurality of measurement operations on the specimen, including: (1) a first measurement operation in which the specimen is measured using a first reagent containing a first fluorescent dye for staining leukocytes for classification of leukocytes; (2) a second measurement operation in which the specimen is measured using a second reagent containing a second fluorescent dye for staining cells suspected of being infected with malaria; and (3) a third measurement operation in which the specimen is measured using the first reagent and the second reagent. [Selection Diagram] Figure 1

Inventors

鳥家雄二
藤巻謙一
辻智悠
松葉弘晃
山内将哉

Assignees

シスメックス株式会社

Dates

Publication Date: 20260507
Application Date: 20241021

Claims (20)

A specimen measuring device for measuring specimens collected from a subject, A measurement unit that prepares a measurement sample from the aforementioned sample and reagents and detects an optical signal corresponding to at least the cells in the measurement sample, The analysis unit includes an analysis unit that analyzes the cells in accordance with the measurement of the sample by the measurement unit, The measurement unit measures the sample as follows: (1) A first measurement operation in which the sample is measured using a first reagent containing a first fluorescent dye for staining the leukocytes in order to classify the leukocytes, (2) A second measurement operation in which the sample is measured using a second reagent containing a second fluorescent dye for staining the cells suspected of being infected with malaria, (3) A third measurement operation in which the sample is measured using the first reagent and the second reagent, Multiple measurement operations, including the following, can be selectively performed. Sample measuring device.
The measurement unit includes a plurality of mechanisms for measuring the sample to be measured, The measurement unit shares at least one of the plurality of mechanisms in the first measurement operation, the second measurement operation, and the third measurement operation. A specimen measuring device according to claim 1.
The measurement unit includes an optical detection unit for detecting an optical signal corresponding to the cells in the measurement sample. The measurement unit shares the optical detection unit for the first measurement operation, the second measurement operation, and the third measurement operation. A specimen measuring device according to claim 1.
The measurement unit includes at least one light source for irradiating the sample to be measured with light, The measurement unit shares the at least one light source for the first measurement operation, the second measurement operation, and the third measurement operation. A specimen measuring device according to claim 1.
The measurement unit includes a light source for irradiating the sample to be measured with light of a first wavelength and a light source for irradiating light of a second wavelength. In the first measurement operation, the measurement unit irradiates the sample to be measured with at least one of the first wavelength of light and the second wavelength of light, and in the second measurement operation, irradiates the sample to be measured with the first wavelength of light and the second wavelength of light. A specimen measuring device according to claim 1.
The first wavelength is 315 nm or more and 490 nm or less. The second wavelength is 610 nm or more and 750 nm or less. A specimen measuring device according to claim 5.
The measurement unit includes a suction tube for aspirating the sample supplied to the sample measuring device. The measurement unit uses the suction tube for the first measurement operation, the second measurement operation, and the third measurement operation. A specimen measuring device according to claim 1.
The measurement unit includes a suction tube for aspirating the sample supplied to the sample measuring device, and a pump used to aspirate the sample by the suction tube. The measurement unit uses the suction tube and the pump for the first measurement operation, the second measurement operation and the third measurement operation. A specimen measuring device according to claim 1.
The measurement unit performs the first measurement operation and the second measurement operation in the third measurement operation. A specimen measuring device according to claim 1.
The measurement unit includes a plurality of mechanisms for measuring the sample to be measured, At least one of the aforementioned plurality of mechanisms performs a different operation in each of the first measurement operation and the second measurement operation. A specimen measuring device according to claim 1.
The measurement unit includes an optical detection unit for detecting an optical signal corresponding to the cells in the measurement sample. The measurement time by the optical detection unit in the first measurement operation is different from the measurement time by the optical detection unit in the second measurement operation. A specimen measuring device according to claim 1.
The measurement unit includes a sample preparation unit for preparing the measurement sample from the sample and the reagent, The sample preparation unit prepares a first measurement sample from the sample and the first reagent in the first measurement operation, and prepares a second measurement sample from the sample and the second reagent in the second measurement operation. A specimen measuring device according to claim 1.
The sample preparation unit prepares a first measurement sample from the sample, the first reagent, and the first hemolytic reagent in the first measurement operation, and prepares a second measurement sample from the sample, the second reagent, and the second hemolytic reagent in the second measurement operation. A specimen measuring device according to claim 12.
The measurement unit measures the sample for the purpose of counting and classifying the white blood cells in the first measurement operation. A specimen measuring device according to claim 1.
In the second measurement operation, the measurement unit measures the sample for the purpose of counting the cells suspected of being infected with malaria and counting the white blood cells. A specimen measuring device according to claim 1.
In the third measurement operation, the measurement unit prepares a common measurement sample for classifying the leukocytes and counting the cells suspected of being infected with malaria, and detects the optical signal corresponding to the cells in the measurement sample. A specimen measuring device according to claim 1.
The measurement unit includes an optical detection unit for detecting an optical signal corresponding to the cells in the measurement sample, and an electrical detection unit for detecting an electrical signal corresponding to the cells in the measurement sample. The measurement unit uses both the optical detection unit and the electrical detection unit in the first measurement operation and the second measurement operation. A specimen measuring device according to claim 1.
The measurement unit includes an optical detection unit for detecting an optical signal corresponding to the cells in the measurement sample, and an electrical detection unit for detecting an electrical signal corresponding to the cells in the measurement sample. The measurement unit does not use the electrical detection unit in the second measurement operation. A specimen measuring device according to claim 1.
The analysis unit analyzes the cells using different clustering methods in the first and second measurement operations. A specimen measuring device according to claim 1.
The analysis unit, in the analysis corresponding to the first measurement operation, classifies the white blood cells into multiple subgroups. A specimen measuring device according to claim 1.

Description

This invention relates to a specimen measuring device for measuring specimens. Specimen analyzers are known that can detect blood cells such as white blood cells and red blood cells infected with malaria. For example, Patent Document 1 describes an analyzer that acquires scattered light signals, a first fluorescence signal corresponding to the first dye, and a second fluorescence signal corresponding to the second dye from a measurement sample prepared by mixing a blood sample, a hemolytic agent, a first dye capable of staining white blood cells, and a second dye capable of staining infected red blood cells. This analyzer acquires white blood cell optical information and infected red blood cell optical information in a single test. This analyzer can simultaneously detect white blood cell parameters and infected red blood cell parameters in a single test. This reduces the amount of blood used for testing compared to preparing two measurement samples for each parameter, thus lowering testing costs. International Publication No. 2022/115982 Figure 1 is a perspective view showing the configuration of a sample measuring device according to Embodiment 1.Figure 2 is a block diagram showing the functional configuration of the measurement unit according to Embodiment 1.Figure 3 shows the configuration of the optical detection unit according to Embodiment 1.Figure 4 shows the configuration of the electrical detection unit and the hemoglobin detection unit according to Embodiment 1.Figure 5 shows a configuration for aspirating and discharging a sample via a suction tube according to Embodiment 1.Figure 6 shows the configuration of the fluid circuit connected to the chamber and the optical detection unit according to Embodiment 1.Figure 7 shows the configuration of a fluid circuit connected to a chamber, an electrical detection unit, and a hemoglobin detection unit according to Embodiment 1.Figure 8 is a block diagram showing the functional configuration of the transport unit and the analysis unit according to Embodiment 1.Figure 9 shows an example of the relationship between discrete components, measurement mode, and measurement items according to Embodiment 1.Figure 10 is a diagram showing the configuration of the menu screen according to Embodiment 1.Figure 11 is a diagram showing the configuration of the measurement type switching dialog, manual measurement dialog, and measurement unit information display area according to Embodiment 1.Figure 12 is a diagram showing the configuration of the measurement order registration screen according to Embodiment 1.Figure 13 is a diagram illustrating the measurement order registration screen when normal mode is selected as the measurement order according to Embodiment 1.Figure 14 is a diagram illustrating the measurement order registration screen when malaria mode is selected as the measurement order according to Embodiment 1.Figure 15 is a diagram illustrating the measurement order registration screen when multimode is selected as the measurement order according to Embodiment 1.Figure 16 shows the configuration of the scattergram WDF and scattergram WNR according to Embodiment 1.Figure 17 shows the configurations of scattergram M and scattergram RET according to Embodiment 1.Figure 18 shows the configuration of the scattergram PLT-F according to Embodiment 1.Figure 19 is a diagram showing the configuration of the analysis result display screen according to Embodiment 1.Figure 20 is an example of the analysis result display screen according to Embodiment 1, when the measurement mode and discrete components are set as shown in Figure 13.Figure 21 is a diagram illustrating the analysis result display screen according to Embodiment 1, when the measurement mode and discrete components are set as shown in Figure 14.Figure 22 is an example of the analysis result display screen according to Embodiment 1, when the measurement mode and discrete components are set as shown in Figure 15.Figure 23 is a flowchart showing the processing performed by the control unit of the analysis unit according to Embodiment 1.Figure 24 is a diagram showing the configuration of the reflex settings screen according to Embodiment 2.Figure 25 is a flowchart showing the processing performed by the control unit of the analysis unit according to Embodiment 2.Figure 26 is a flowchart showing the processing performed by the control unit of the analysis unit when the measurement operation is selectively performed based on other information about the subject, according to Modification Example 1.Figure 27 shows the configuration of the fluid circuit connected to the chamber and the optical detection unit according to Modification Example 2.Figure 28 shows the configuration of the fluid circuit connected to the chamber and the optical detection unit according to Modification Example 3.Figure 29 shows the configuration of the scattergram WDF according to modification example 4.Figure 30 shows the configuration of the