JP-2026075206-A - Reagents for measuring biological samples containing disulfides and methods for suppressing the influence of thiols

Abstract

[Problem] To provide a method for suppressing the effects of thiols in biological sample measurement reagents, and a biological sample measurement reagent utilizing this method. [Solution] The biological sample measurement reagent contains disulfide. This biological sample measurement reagent can suppress the effects of thiols. [Selection Diagram] None

Inventors

• 石井 悠貴
• 田村（門馬） 美奈子
• 中尾 友作

Assignees

• 株式会社シノテスト

Dates

Publication Date

20260508

Application Date

20241022

Claims (11)

1. A reagent for measuring biological samples, characterized by containing disulfide.
2. The reagent according to claim 1, wherein the disulfide is at least one of the following: 5,5'-dithiobis(2-nitrobenzoic acid), bis(2-hydroxyethyl) disulfide, cystamine dihydrochloride, and 33'-dithiodipropionic acid.
3. The reagent according to claim 1 or 2, wherein the presence of a disulfide is for the purpose of suppressing the effect of thiols.
4. The reagent according to claim 1 or 2, wherein the biological sample measurement reagent is a biochemical test reagent.
5. The reagent according to claim 3, wherein the biological sample measurement reagent is a biochemical test reagent.
6. The reagent according to claim 4, wherein the biological sample measurement reagent is a zinc measurement reagent.
7. The reagent according to claim 5, wherein the biological sample measurement reagent is a zinc measurement reagent.
8. A method for suppressing the influence of thiols in biological sample measurement reagents, characterized by the inclusion of disulfide.
9. The method according to claim 8, wherein the disulfide is at least one of the following: 5,5'-dithiobis(2-nitrobenzoic acid), bis(2-hydroxyethyl) disulfide, cystamine dihydrochloride, and 33'-dithiodipropionic acid.
10. The method according to claim 8 or 9, wherein the biological sample measurement reagent is a biochemical test reagent.
11. The method according to claim 10, wherein the biological sample measurement reagent is a zinc measurement reagent.

Description

This invention relates to a biological sample measurement reagent characterized by containing disulfide to suppress the effects of thiols. Furthermore, this invention relates to a method for suppressing the effects of thiols in a biological sample measurement reagent, characterized by the inclusion of disulfide. This invention is particularly useful in fields such as chemistry, life sciences, analytical science, and clinical testing. Various metals exist within the body, each performing diverse functions. Therefore, measuring metal concentrations in biological samples and observing their fluctuations is essential for disease diagnosis, treatment, early detection, and prevention, and is widely practiced in hospitals and testing facilities. For example, zinc deficiency is known to cause symptoms such as developmental disorders and taste disorders; therefore, serum zinc concentration measurements may be performed when these symptoms are observed. Methods for measuring zinc concentration include atomic absorption spectrometry, ICP emission spectrometry, and colorimetric analysis. Among these, colorimetric analysis using chelating chromogens measures zinc concentration by utilizing the change in absorbance that occurs when zinc forms a chelate with a chelating chromogen such as 2-(5-bromo-2-pyzylyl azo)-5(N-propyl-N-sulfopropylamino)phenol sodium (sometimes abbreviated as 5-Br-PAPS) or 2-(5-nitro-2-pyzylyl azo)-5(N-propyl-N-sulfopropylamino)phenol sodium (sometimes abbreviated as nitro-PAPS). This method allows for the measurement of zinc concentration in biological samples using general-purpose automated analyzers, even without an atomic absorption spectrophotometer, and is used in hospital laboratories and other settings. Incidentally, when using biological materials as samples for measurements, the sample may contain numerous substances that are not the target of measurement. These untargeted substances are sometimes called coexisting substances, and there is a concern that they may adversely affect the measurement. If such adverse effects occur, problems such as the inability to accurately measure the concentration of the target substance may arise. This type of effect is called the influence of coexisting substances, and hemoglobin is known to be one such substance. To prevent such effects, methods for suppressing interference (see Patent Document 1), characterized by the addition of carboxylic acids or their salts, and/or anionic surfactants, and influence avoidance agents (see Patent Document 2), which use nonionic surfactants as active ingredients, are known. Japanese Patent Publication No. 2020-048431Japanese Patent Publication No. 2015-119729 The present invention will be described in detail below. The following embodiments are illustrative examples for illustrating the present invention and are not intended to limit the invention to these embodiments only. The present invention can be implemented in various forms without departing from its essence. 1. Overview When using biological materials as samples for measurements, the sample may contain many substances that are not the target of measurement. These substances, sometimes called coexisting substances, may have adverse effects on the measurement. If such adverse effects occur, problems such as the inability to accurately measure the concentration of the target substance may arise. This type of effect is called the effect of coexisting substances, and hemoglobin is known to be one of the substances that have this effect. While conducting research on reagents for measuring biological samples, the inventors encountered a phenomenon in which, when performing a sample recovery test using a sample containing a large amount of thiols as coexisting substances, the measured value of the target substance was lower than the expected value. Such biological sample measurement reagents have problems such as the inability to obtain accurate measurement values or the possibility that measurement may not be possible at all when measuring samples containing a large amount of thiols as coexisting substances. The inability to obtain accurate measurements, or even the inability to perform measurements at all, is a serious problem that can lead to misdiagnosis or delays in disease diagnosis. In the field of biological sample measurement reagents, there is a need for reagents that can provide accurate measurements. Therefore, regarding the influence of thiols, there is a demand for biological sample measurement reagents that do not exhibit such phenomena. As a result of diligent research into the above-mentioned problems and their solutions, the inventors have discovered that the influence of thiols can be suppressed by including certain compounds in the biological sample measurement reagent. This invention was made based on these findings. 2. Addition and Recovery Test The addition and recovery test in this invention is a test to confirm whether the amount of the target