JP-2026076164-A - Adipose tissue matrix containing tropoelastin

Abstract

[Problem] To provide a tissue product manufactured from adipose tissue and a method for manufacturing the same. [Solution] A method for producing a tissue product is provided, comprising the steps of: selecting adipose tissue; processing the tissue to remove substantially all cellular material from the tissue; suspending the tissue in a liquid to form a suspension containing 2-4% by weight of solids; and freezing and drying the suspension to form a porous sponge. [Selection Diagram] Figure 1

Inventors

• シュ，フゥイ
• パスティノ，アレクサンドラ
• ファン，キャリー

Assignees

• ライフセル コーポレーション

Dates

Publication Date

20260511

Application Date

20251225

Priority Date

20200403

Claims (20)

1. A method for manufacturing tissue products, The step of selecting adipose tissue, The steps include processing the tissue to remove virtually all cellular material from it, The steps include suspending the tissue in a liquid to form a suspension containing 2-4% by weight of solids, A method characterized by comprising the steps of freezing and drying a suspension to form a porous sponge.
2. In the method according to claim 1, A method characterized by further comprising the step of crosslinking a porous sponge.
3. In the method of claim 2, A method characterized in that crosslinking is performed using a dehydrothermal process.
4. In the method according to claim 3, A method characterized by further comprising a step of performing a chemical crosslinking process.
5. In the method according to claim 1, A method characterized in that a porous sponge has a desired thickness at least in the thickest part of the sponge, and that thickness exceeds 10.0 cm.
6. In the method according to claim 1, A method further comprising the step of adding a suspension to a mold.
7. In the method according to claim 6, A method characterized in that the mold is in the shape of a rounded breast implant or a teardrop-shaped breast implant.
8. In the method according to claim 4, A method characterized in that the chemical crosslinking step includes at least one of glutaraldehyde, genepine, carbodiimide, and diisocyanate.
9. In the method according to claim 4, A method characterized by crosslinking, which includes heating a porous sponge.
10. In the method according to claim 9, A method characterized by heating a porous sponge in a vacuum.
11. In the method according to claim 10, A method characterized by heating a porous sponge to a temperature range of 70°C to 120°C.
12. In the method according to claim 4, A method characterized in that a porous sponge is crosslinked so that the material maintains a stable three-dimensional structure when it comes into contact with an aqueous environment.
13. In the method according to claim 12, A method characterized in that the aquatic environment is the body of a mammal.
14. It is an organizational product, A tissue product comprising a breast implant, wherein the implant comprises a construct of a cell-free adipose tissue matrix that is homogenized, dried, and stabilized to form a suspension, the implant having at least one dimension of at least 5 cm, and comprising tropoelastin.
15. In the tissue product according to claim 14, The implant is a tissue product characterized by having at least one dimension of at least 8 cm.
16. In the tissue product according to claim 14, A tissue product characterized by the rounded shape of the breast implant.
17. In the tissue product according to claim 14, A tissue product characterized by the implant having a teardrop shape as a breast implant.
18. A method for manufacturing tissue products, The step of selecting adipose tissue, The steps include processing the tissue to remove virtually all cellular material from it, The steps include suspending the tissue in a liquid to form a suspension, The step of adding tropoelastin to the suspension, A method characterized by comprising the steps of freezing and drying a suspension to form a porous sponge.
19. In the method according to claim 18, A method characterized by further comprising the step of crosslinking a porous sponge.
20. In the method according to claim 19, A method characterized in that crosslinking is performed using a dehydrothermal process.

Description

This disclosure relates to tissue products, and more specifically, to extracellular tissue matrices derived from adipose tissue and containing tropoelastin. This application claims priority to U.S. Provisional Application No. 63/004,794, filed on 3 April 2020 pursuant to Section 119 of the U.S. Patent Act, which is incorporated herein by reference in its entirety. Various tissue-derived products are used to regenerate, repair, or otherwise treat diseased or damaged tissues and organs. Such products may include tissue grafts and/or processed tissues (e.g., cell-free tissue matrices from skin, intestines, or other tissues, with or without cell seeding). Such products generally have properties determined by the tissue source (i.e., the type of tissue and the animal from which it originates) and the processing parameters used to manufacture the tissue product. Since tissue products are often used for surgical applications and/or tissue replacement or augmentation, the products must support tissue growth and regeneration as desired at the selected transplant site. This disclosure provides adipose tissue products that can improve tissue growth and regeneration for various applications, such as breast implants. The tissue products may further contain tropoelastin to improve biological or mechanical properties. The products may be crosslinked to maintain a desired shape when transplanted. According to certain embodiments, a method for producing a tissue product is provided. This method may include the steps of: selecting adipose tissue; mechanically processing the adipose tissue to reduce its size; processing the mechanically processed tissue to remove substantially all cellular material from it; suspending the tissue in a liquid to form a suspension; and drying the suspension in a mold to form a porous sponge. In some embodiments, the suspension is dried and subjected to a treatment such as dehydrothermal crosslinking. The tissue product may contain tropoelastin. In various embodiments, adipose tissue is treated to control specific mechanical properties. For example, the treated tissue may be crosslinked to provide a stable three-dimensional structure. Additionally or alternatively, the solid content of the sponge or suspension can be controlled, as will be described in more detail below. Furthermore, in some embodiments, tropoelastin may be added to the composition before forming the suspension. Furthermore, this specification also provides organizational products created through the disclosure process. In some embodiments, the tissue product comprises a decellularized extraadipocyte tissue matrix, the tissue matrix being formed into a predetermined three-dimensional shape, and the tissue matrix being partially crosslinked to maintain the three-dimensional shape. The product may contain a desired amount of tropoelastin. Furthermore, this specification also provides tissue products including breast implants. These implants may include adipose tissue matrix formed to have a desired set of mechanical properties controlled by the proportion of crosslinking and/or solids. The product may further contain tropoelastin. Furthermore, this specification also provides tissue products containing an injectable fat matrix or particulate fat matrix. This implant may be at least partially crosslinked solids. The product may further contain tropoelastin. Furthermore, this specification provides a therapeutic method comprising the steps of selecting a tissue site and implanting the tissue product disclosed herein into the tissue site. Figure 1 is a flowchart outlining the process for manufacturing an adipose tissue matrix sponge according to a specific embodiment.Figure 2 is a side view of a biological breast implant according to a specific embodiment.Figure 3A is a perspective view of the configuration of a breast implant according to a particular embodiment. Figure 3B is a perspective view of another configuration of the breast implant according to a particular embodiment. Figure 3C is a perspective view of another configuration of the breast implant according to a particular embodiment.Figure 4 shows the implantation of a system for surgical breast surgery according to a specific embodiment.Figures 5A to 5G are tissue images showing the effect of EDC crosslinking on lipid synthesis.Figure 6A is a bar graph showing the effect of fat matrix solids on compressive strength. Figure 6B is a bar graph showing the effect of fat matrix solids on recovery rate. Figure 6C is a bar graph showing the effect of fat matrix solids on elasticity. Figure 6D is a bar graph showing the effect of fat matrix solids on elastic modulus.Figure 7A is a bar graph showing the effect of changes in crosslinking agent content or tropoelastin content on the compressive strength of the adipose matrix product. Figure 7B is a bar graph showing the effect of changes in crosslinking agent content or tropoelastin content on the recovery rate of the adipose matrix p