JP-2026076382-A - Antibacterial and antioxidant composition containing grape callus culture solution as an active ingredient

Abstract

[Problem] To provide an antioxidant and antibacterial composition containing plant-derived active ingredients. [Solution] The present invention relates to an antibacterial and antioxidant composition containing grape callus culture solution containing epsilon(ε)-viniferin, delta(δ)-viniferin, and resveratrol as active ingredients. Specifically, the grape callus, its culture solution, and its extract of the present invention are non-cytotoxic, exhibit significant antioxidant and antibacterial effects against acne bacteria, and have particularly remarkable antioxidant and antibacterial effects compared to pure vinylin. Therefore, the grape callus, its culture solution, and its extract can be usefully used as active ingredients in antioxidant and antibacterial compositions. [Selection Diagram] Figure 1

Inventors

• ピ、ジェホ

Assignees

• アイエヌジーアル インク

Dates

Publication Date

20260511

Application Date

20260224

Priority Date

20210914

Claims (10)

1. An antimicrobial pharmaceutical composition containing grape callus culture solution or an extract of grape callus culture solution as an active ingredient.
2. The antimicrobial pharmaceutically acceptable composition according to claim 1, characterized in that the grape callus is derived from one or more parts selected from the group consisting of grape flowers, leaves, stems, branches, fruits, and roots.
3. The antimicrobial pharmaceutically active composition according to claim 1, characterized in that it has antimicrobial activity against Propionibacterium acnes.
4. The antimicrobial pharmaceutical composition according to claim 1, characterized in that the composition has bacteriostatic and bactericidal activity.
5. A pharmaceutical composition for the prevention and treatment of acne, containing grape callus culture solution or an extract of grape callus culture solution as an active ingredient.
6. The pharmaceutical composition for preventing and treating acne according to claim 5, characterized in that the composition has antibacterial activity against Propionibacterium acnes.
7. The pharmaceutical composition for the prevention and treatment of acne according to claim 5, characterized in that the composition has bacteriostatic and bactericidal activity against Propionibacterium acnes.
8. An antibacterial cosmetic composition containing grape callus culture solution or an extract of grape callus culture solution as an active ingredient.
9. A cosmetic composition for preventing and improving acne, containing grape callus culture solution or an extract of grape callus culture solution as an active ingredient.
10. A composition for the prevention, improvement, or treatment of bacterial infection, containing grape callus culture solution or an extract of grape callus culture solution as an active ingredient.

Description

This invention relates to an antibacterial and antioxidant composition containing grape callus culture solution as an active ingredient, including epsilon(ε)-viniferin, delta(δ)-viniferin, and resveratrol. In the global shift from an industry-centered society to a service-centered society, human health has become the most pressing issue. While the rapid expansion of petroleum-based industrial societies brought convenience, the after-effects of this rapid development not only polluted living environments but also harmed human health. In response, there is a growing interest in and demand for environmentally friendly materials, particularly in everyday products. Oxygen is an essential element for sustaining life, used in the human body to produce energy through the process of respiration. However, oxygen doesn't just provide us with energy. During the process of combining oxygen with food in the body, it creates harmful oxygen species called reactive oxygen species. These reactive oxygen species act as a cause of disease in our bodies, and recently, the medical community has identified reactive oxygen species as the main culprit behind human aging. Therefore, steady progress is being made in developing natural antioxidants that can protect the human body from the toxicity of reactive oxygen species. Acne is a skin condition that occurs in most people of all ages, forming pustules or cysts. Acne develops due to a combination of factors, including excessive sebum secretion, hormonal imbalances, abnormal keratinization within hair follicles, and the proliferation and inflammatory response of Propionibacterium acnes. Propionibacterium acnes, the bacterium that causes acne, is an anaerobic skin flora that proliferates within hair follicles. Lipase hydrolyzes triglycerides in sebum, producing free fatty acids. This stimulates the hair follicles, generating chemical factors that trigger an inflammatory response. While antibiotics are the most common treatment for acne caused by Propionibacterium acnes infection, their effectiveness can decrease due to antibiotic resistance. Therefore, research into herbal substances that do not cause side effects or resistance in acne treatment is actively underway. On the other hand, numerous studies have attempted to produce a variety of useful substances (e.g., secondary metabolites) from plants using biotechnology. Among these, plant cell culture is the most effective production method. This method can effectively produce secondary metabolites through the use of culture media, a suitable culture environment, and various inducer (elictor) treatments. Furthermore, it is commercially important because it allows for the continuous and stable mass production of useful plant-derived substances without being subject to the constraints of cultivation or the natural environment (highly toxic pesticides, heavy metal contamination, seasonal influence, climatic conditions, etc.). Furthermore, as related prior art, Korean Registered Patent No. 10-1253374 (Cosmetic Composition for Improving Acne-Prone Skin) utilizes kimchi lactic acid bacteria fermentation extract and soybean fermentation extract as a cosmetic composition for improving acne-prone skin, and Korean Registered Patent No. 10-0879032 (Antibacterial, Antioxidant, and Anti-inflammatory Inhibitor with Natural Antibacterial and Anti-inflammatory Effects and Cosmetic Composition Containing the Same) provides a cosmetic composition with antibacterial effects using a mixed extract of the herbal medicine materials licorice, rhizome, rhizome, astragalus, coptis japonica, sophora, rhizome, and gromwell. However, the antibacterial and antioxidant effects of grape callus against acne bacteria are not known. Therefore, the inventors have strived to develop a method for mass-producing useful plant-derived substances and to develop functional cosmetics, health foods, and pharmaceutical materials utilizing these substances. As a result, the grape callus culture solution of the present invention exhibits significant antioxidant and antibacterial effects, particularly those significantly greater than those of pure viniferin. Therefore, the present invention was completed by demonstrating that the aforementioned grape callus culture solution can be usefully used as a naturally derived antioxidant and antibacterial pharmaceutical composition, cosmetic composition, and health food. Figure 1 shows the cytotoxicity of vinylin and grape callus culture medium (sample name A) in MNT-1 cells. To compare with vinylin, the grape callus culture medium (sample name A) was diluted according to the epsilon vinylin content standard and treated in wells.Figure 2 shows the cytotoxicity of vinylin and grape callus culture medium (sample name A) in Raw 264.7 cells. To compare with vinylin, the grape callus culture medium (sample name A) was diluted according to the epsilon vinylin content standard and treated in the wells.Figure 3 shows the results of DPPH analysis to