JP-2026514387-A - Automated data storage system

Abstract

[Challenge] A system for data storage. [Solution] A system for data storage is described, comprising components for receiving, encoding, synthesizing, storing, reading, sequencing, and decoding digital information stored in polynucleotides and libraries of polynucleotides. [Selection Diagram] Figure 2B

Inventors

• ルドフ，ロジャー
• トッパーニ，ドミニク
• ディガンズ，ジェームズ
• ワトソン，マーク
• バンヤイ，ウィリアム

Assignees

• アトラス データ ストレージ，インコーポレイテッド

Dates

Publication Date

20260511

Application Date

20240328

Priority Date

20230328

Claims (18)

1. A system for data storage, A computing system comprising at least one processor and instructions executable by the at least one processor for performing one or more operations, Receiving digital information, A computing system and a modular rack-mount synthesis unit comprising encoding digital information into one or more nucleic acid sequences and synthesizing a library of polynucleotides corresponding to the nucleic acid sequences, Computer controller, One or more reservoirs, A flow cell block containing one or more flow cells, Extraction stage, Post-processing unit, Storage unit, A system including a modular rack-mount composite unit, including robot interference and/or a rack interface.
2. The system according to claim 1, wherein the at least one flow cell block comprises at least 12 flow cells.
3. The one or more flow cells described above are The system according to claim 1, comprising one or more solid supports configured for synthesizing a library of the polynucleotides, and one or more ports for exchanging gases, synthesis reagents, and/or extracted polynucleotides.
4. The modular rack-mount synthesis unit includes the extraction stage and/or the post-processing unit, and the extraction stage and/or the post-processing unit is The system according to claim 1, comprising an energy supply chain and one or more ports for exchanging synthetic reagents and/or extracted polynucleotides.
5. The modular rack-mount composite unit includes the storage unit, and the storage unit is Storage plate, The system according to claim 1, comprising a robotic interface configured to move a storage plate, and an access port.
6. The modular rack-mount composite unit includes the rack interface, and the rack interface is power supply, Rack reagent bulkhead, Synthesis unit reagent bulkhead, One or more reservoirs, The system according to claim 1, comprising an energy supply chain and a pilot valve bank.
7. The system according to claim 1, wherein the storage unit provides the polynucleotide library to a sequencing unit configured to sequence the polynucleotide library.
8. It further includes one or more sensors, and the one or more sensors are The state is detected, and the state includes temperature, pressure, humidity, voltage changes, current changes, capacitance, conductivity, storage plate position, liquid volume, flow rate, and/or presence of liquid. The system according to claim 1, configured to report the aforementioned state to the computing system.
9. The system according to claim 8, wherein the modular rack-mount synthesis unit includes the at least one flow cell block, and the one or more sensors are further configured to pause synthesis in the one or more flow cells or the at least one flow cell block.
10. The system according to claim 9, wherein the system is a first system for data storage, and the computing system is configured to route encoded data from (i) a suspended flow cell or (ii) a suspended flow cell block to an active flow cell, an active flow cell block, or a second system for data storage.
11. The computing system further includes a cache, where digital information is held in the cache until a polynucleotide encoding the digital information is stored, according to claim 1.
12. An assembly for storing information, A plurality of compartments containing a first material, each compartment configured to receive a plurality of polynucleotides that encode information, It includes a base plate containing a second material and a cover plate containing a third material, The coefficient of thermal expansion (CTE) of the first material, the second material, or both thereof is smaller than that of the third material. assembly.
13. The assembly according to claim 12, wherein the plurality of compartments are arranged in an array on the base plate.
14. The assembly according to claim 13, wherein the base plate, the cover plate, or both include a plurality of concave features, and each section is at least partially located within a concave feature.
15. The assembly according to claim 12, wherein the first material comprises a borosilicate.
16. The assembly according to claim 12, wherein the second material has a specific heat capacity of approximately 0.5 J/k-°C to 2.5 J/k-°C.
17. The third material is, CTE of approximately 15 μm/m-°C to 20 μm/m-°C, The assembly according to claim 12, comprising a thermal conductivity of approximately 15 W/m-K to approximately 20 W/m-K, and/or a specific heat capacity of approximately 0.5 J/g-°C.
18. The assembly according to claim 12, wherein the third material includes stainless steel.

Description

Cross-reference of related applications [0001] This application claims the benefits and priority of U.S. Provisional Patent Application No. 63/492,593 filed on 28 March 2023 and U.S. Provisional Patent Application No. 63/514,227 filed on 18 July 2023, the contents of which are incorporated herein by reference in their entirety. All publications, patents, and patent applications referenced herein are incorporated by reference to the same extent as each individual publication, patent, or patent application is specifically and individually indicated as being incorporated by reference. background [0002] DNA is an attractive data storage medium given its superior density, stability, energy efficiency, and lifespan compared to current storage methods. However, automated systems may involve processes that perform several different components, such as coding, writing/synthesis, storage, and reading/sequencing. Therefore, there is a need to develop systems that can perform these functions. [0009]This flowchart shows an exemplary workflow for an automated data storage system using nucleic acids including a synthetic module according to an aspect of the present disclosure.[0010] This flowchart shows the workflow of an automated data storage system using nucleic acids, including a storage module. In some examples, these steps are performed by a robotic stage according to aspects of the present disclosure.[0011] A flowchart shows an exemplary system architecture workflow at the server rack level for an automated data storage system (as described herein) using nucleic acids, including firmware, a control system (such as a network interface controller), and a virtual data path, according to aspects of the present disclosure. In some aspects, data may move to a server rack for storage and verification, and the server rack may transmit data to an external interface for database management.[0012] A figure showing an exemplary standardized DNA synthesis unit (DSU) according to an aspect of the present disclosure, including data/addressing, power, and reagent/DNA input/removal (flow cell, flow cell block, or entire data storage unit), and including a table describing the dimensions and chip density of an exemplary 6U module.[0013] This is a system-level diagram illustrating an exemplary automated data storage system using nucleic acids. It shows modules for reagents, rack mounts, synthesis, post-processing (e.g., deprotection, drying, PCR, purification), control, power distribution, and status indicators according to aspects of the present disclosure.[0014] This figure shows an exemplary rack unit for data storage, displaying a DNA data storage system unit extending from a rack unit according to an aspect of the present disclosure.[0015] This figure shows the mechanical layout of the DNA data storage system unit shown in Figure 3A according to an aspect of the present disclosure.[0016] This figure shows a schematic front/transparent view of an exemplary DNA data storage system unit configured for use in a rack unit as shown in Figure 3A, according to an aspect of the present disclosure.[0017] This figure shows a schematic diagram of the back panel of an exemplary DNA data storage system unit according to an aspect of the present disclosure.[0018] Two figures illustrating the mechanical layout of an extraction stage of an exemplary DNA data storage system unit according to an aspect of the present disclosure. The top figure shows an extraction stage in which the storage chamber is located in a first position for distribution at the front of the stage. The bottom figure shows an extraction stage in which the storage chamber is moved to a second position at the rear of the stage for waste/washing. These two positions are shown for illustrative purposes only, and in some examples the chamber is moved to any position associated with the flow cell block.[0019] This figure shows a side view of the fluid pathway of an exemplary DNA data storage system unit, including reagent delivery, according to an aspect of the present disclosure.[0020] This figure shows a top view of the fluid pathway of an exemplary DNA data storage system unit, including reagent delivery, according to an aspect of the present disclosure.[0021] This figure shows a schematic diagram of the fluid path of an exemplary flow cell block including a control valve.[0022] This figure shows an exemplary flow cell block device illustrating fluid input. Twelve flow cell units are shown as an example only.[0023] Three schematic diagrams of an exemplary flow cell block for synthesis, including a filling, washing, and drying process using a Barcart valve (6712 series, 24V), according to an aspect of the present disclosure.[0024] Three schematic diagrams of an exemplary flow cell block for extraction, including a washing, filling, and extraction process using a Barcart valve (6712 series, 24V), according to an aspect of the present disclosure.[0025] This figure shows a schematic