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JP-7855063-B2 - Copolymers, polymer membranes, measuring devices, and measuring carriers

JP7855063B2JP 7855063 B2JP7855063 B2JP 7855063B2JP-7855063-B2

Inventors

  • 栗岡 秀治
  • 小村 ちさと
  • 安藤 剛
  • 網代 広治

Assignees

  • 京セラ株式会社

Dates

Publication Date
20260507
Application Date
20230426
Priority Date
20220426

Claims (8)

  1. A copolymer, Two hydrophilic blocks A, each containing at least one structural unit (a) from among the structural units (a) represented by the following formulas (1) to (6), (In the formula, R1 to R7 are each independently H or CH3 , X1 to X4 are each independently O or NH, p1 to p5 are each independently integers between 1 and 3, and q1 to q3 are each independently integers between 1 and 5.) Between the two hydrophilic blocks A, there is a hydrophobic block B which includes at least one structural unit (b) from among the structural units (b) represented by the following formulas (7) and (8), (In the formula, R8 and R10 are each independently H or CH3 , R9 , R11 , and R12 are each independently alkyl groups having 1 to 6 carbon atoms, X5 and X6 are O or NH, and r is an integer between 1 and 3.) The hydrophobic block B has disulfide bonds between the structural units (b), A copolymer in which the ratio of moles of structural unit (a) to moles of structural unit (b) contained in the copolymer is 0.20 or more and 2.5 or less .
  2. The copolymer according to claim 1, wherein the proportion of the structural unit (b) constituting the hydrophobic block B contained in the copolymer is 30 mol% or more and 80 mol% or less.
  3. The copolymer according to claim 1 or 2, wherein the proportion of the structural unit (a) constituting the hydrophilic block A contained in the copolymer is 20 mol% or more and 70 mol% or less.
  4. The copolymer according to claim 1 or 2 , wherein at least one end of the copolymer has a thiol group or a dithioester group.
  5. The copolymer according to claim 1 or 2 , wherein the two hydrophilic blocks A have the same structure as each other.
  6. The copolymer according to claim 1 or 2 , wherein the two hydrophilic blocks A have a number-average degree of polymerization of 100 or more.
  7. The copolymer according to claim 1 or 2 , wherein the two hydrophilic blocks A have a combined number-average degree of polymerization of 30 or more.
  8. The copolymer according to claim 1 or 2 , wherein the hydrophobic block B has a number-average degree of polymerization of 25 or more.

Description

This disclosure relates to copolymers used to form polymer films used in measuring devices. Furthermore, this disclosure relates to polymer films obtained using such copolymers, and measuring devices or measuring carriers equipped with such polymer films. As an example of a measuring device for measuring the concentration of a target substance (e.g., biomolecules), a surface acoustic wave sensor is disclosed in Patent Document 1. Such a measuring device includes a detection unit on which a substance that interacts with the target substance contained in the sample (e.g., an antibody) is immobilized. A polymer film is often formed on the detection unit in order to immobilize the substance that interacts with the target substance contained in the sample. Japanese Patent Application Publication No. 2008-286606 A copolymer according to one embodiment comprises two hydrophilic blocks A, each containing at least one structural unit (a) from among the structural units (a) represented by the following formulas (1) to (6), (In the formula, R1 to R7 are each independently H or CH3 , X1 to X4 are each independently O or NH, p1 to p5 are each independently integers between 1 and 3, and q1 to q2 are each independently integers between 1 and 5.) Between the two hydrophilic blocks A, there is a hydrophobic block B which includes at least one structural unit (b) from among the structural units (b) represented by the following formulas (7) and (8), (In the formula, R8 and R10 are each independently H or CH3 , R9 , R11 , and R12 are each independently alkyl groups having 1 to 6 carbon atoms, X5 and X6 are O or NH, and r is an integer between 1 and 3.) The hydrophobic block B is a copolymer having disulfide bonds between the structural units (b). A polymer film according to one embodiment comprises a hydrophilic block A containing at least one structural unit (a) from among the structural units (a) represented by the following formulas (1) to (6), (In the formula, R1 to R7 are each independently H or CH3 , X1 to X4 are each independently O or NH, p1 to p5 are each independently integers between 1 and 3, and q1 to q2 are each independently integers between 1 and 5.) A hydrophobic block B comprising at least one structural unit (b) from among the structural units (b) represented by the following formulas (7) and (8), (In the formula, R8 and R10 are each independently H or CH3 , R9 , R11 , and R12 are each independently alkyl groups having 1 to 6 carbon atoms, X5 and X6 are O or NH, and r is an integer between 1 and 3.) The polymer film comprises a copolymer having a thiol group or a dithioester group at the end of the hydrophobic block B opposite to the side bonded to the hydrophilic block A. This is a schematic diagram showing an example of a measuring device related to this disclosure.This is a plan view showing the sensors included in the above measuring device.Figure 1 is a conceptual diagram showing the polymer contained in the detection unit 23.This is a plan view showing an example of a measuring carrier related to this disclosure.This graph shows the relationship between the unit ratio (CBMA1/BMA) and the amount of non-specific adsorption in the triblock copolymer according to this disclosure.This graph shows the relationship between the average degree of polymerization of CBMA1 in the triblock copolymer according to this disclosure and the amount of non-specific adsorption. [Embodiment 1] The sensors provided in the measuring device according to this disclosure will be described below, with appropriate use of the drawings. Figure 1 shows a schematic of the sensor 2 of the measuring device 100 according to this embodiment. The measuring device 100 can detect a specific substance (first substance) from the object to be measured (sample). The first substance 5 is, for example, a biomolecule. Examples of the biomolecule include proteins, DNA, substrates for enzyme reactions, etc. The measuring device 100 includes a sensor 2 capable of detecting the first substance and a control device 6 capable of controlling the measuring device 100. Sensor 2 can be any sensor that utilizes, for example, elastic waves, QCM (Quartz Crystal Microbalance), SPR (Surface Plasmon Resonance), or FET (Field Effect Transistor). In other words, sensor 2 only needs to be able to convert between electrical signals and elastic waves, QCM, SPR, FET, etc. Sensor 2 according to one embodiment is a sensor that utilizes elastic waves. That is, the measuring device 100 according to one embodiment can detect changes in elastic waves based on the presence of the first substance as changes in electrical signals by using sensor 2. Sensor 2 can be manufactured by conventionally known methods, except for the polymer film 1 described below. In this case, the inspection information included in the identification information may include information specific to sensors that utilize elastic waves, such as the initial phase of the elastic wave and the orientation of