JP-7855666-B2 - A cosmetic composition for soothing irritation and improving moisturizing properties, containing an enzyme-treated and low-temperature aged low-molecular-weight hyaluronic acid hydrolysate as an active ingredient, and a method for producing the hydrolysate thereof.

Inventors

• ミン、チン ウ
• ソ、ウォン サン

Assignees

• フゲル，インコーポレイテッド

Dates

Publication Date

20260508

Application Date

20241121

Priority Date

20231121

Claims (9)

1. Step 1 involves mixing hyaluronic acid with distilled water and proteinase and pectinase as mixed enzymes to carry out an enzymatic reaction to obtain hyaluronic acid treated with mixed enzymes, The process includes a step (step 2) in which the hyaluronic acid treated with mixed enzymes in step 1 is subjected to steam aging, followed by low-temperature aging at 5 to 20°C for 1 to 5 days, A method for producing hyaluronic acid hydrolysate .
2. The manufacturing method according to claim 1, characterized in that the proteinase in step 1 is Flavorzyme, Protamex, α-chymotrypsin, subtilisin Carlsberg, thermolysin, papain, fungal protease, or pepsin .
3. The pectinase in step 1 is Pectinex Ultra SP-L, Viscozyme L The manufacturing method according to claim 1, characterized in that it is L), Ultrazyme AFP, Pectinex Ultra AFP, Pectinex Ultra Clear, and α-herbzyme.
4. The manufacturing method according to claim 1, characterized in that the mixed enzyme contains 1 to 5 parts by weight of proteinase based on 1 part by weight of pectinase.
5. The manufacturing method according to claim 4, characterized in that the mixed enzyme contains 2 to 4 parts by weight of proteinase based on 1 part by weight of pectinase.
6. The manufacturing method according to claim 1, characterized in that the steaming treatment in step 2 is carried out at 70 to 90°C for 2 to 4 hours, and the low-temperature aging is carried out at 14 to 18°C for 2 to 4 days.
7. The manufacturing method according to claim 1, characterized in that the weight-average molecular weight (Mw) of the hyaluronic acid produced by the above manufacturing method is 500 Da or less.
8. A hyaluronic acid hydrolysate with a weight -average molecular weight (Mw) of 500 Da or less, produced by the manufacturing method described in claim 1.
9. A cosmetic composition for soothing skin irritation and moisturizing, comprising a hyaluronic acid hydrolysate produced by the manufacturing method described in claim 1.

Description

This invention relates to a cosmetic composition for irritation relief and moisturizing improvement containing an enzyme-treated and low-temperature aged low-molecular-weight hyaluronic acid hydrolysate as an active ingredient, and to a method for producing the hydrolysate thereof. More specifically, when hyaluronic acid is produced as an enzyme-treated and low-temperature aged hydrolysate, a low-molecular-weight hyaluronic acid hydrolysate with a weight-average molecular weight (Mw) of 500 Da or less is obtained, and its histamine inhibitory ability, NO production inhibitory ability, and moisturizing improvement effects have been confirmed. Hyaluronic acid is a type of glycosaminoglycan that exists in the extracellular matrix and is known to be involved in maintaining cell moisture, storing and diffusing cell growth factors and nutrients. It is synthesized by keratinocytes and fibroblasts. A decrease in hyaluronic acid in the skin is known to cause loss of skin elasticity and an increase in wrinkles. Most hyaluronic acid produced by conventional methods is a high-molecular-weight polysaccharide with a high degree of polymerization of 500,000 Da (500,000 Da = 500 kDa) or more, and therefore cannot penetrate the skin. In other words, while applying hyaluronic acid with a molecular weight of 500,000 Da to or more to the skin can prevent moisture evaporation due to its tendency to absorb moisture from the air, it cannot penetrate the skin. Therefore, it remains on the skin's surface and is easily washed away, making it difficult to sustain the skin's moisturizing effect. For this reason, various attempts to reduce the molecular weight of hyaluronic acid have been reported. Regarding prior art for reducing the molecular weight of hyaluronic acid, Patent Document 1 discloses a method for producing hyaluronic acid oligomers containing ultra-low molecular weight hyaluronic acid. This method involves a deacetylation reaction to produce low molecular weight hyaluronic acid. The document reports that the composition exhibits an effect of reducing and improving wrinkles by promoting fibroblast activity and collagen synthesis. Furthermore, Patent Document 2 relates to a method for producing low-molecular-weight hyaluronic acid by gamma-ray irradiation and hyaluronic acid-degrading enzyme treatment. It provides a method for producing low-molecular-weight hyaluronic acid hydrolysate by treating hyaluronic acid with recombinant protein enzymes and then irradiating it with gamma rays. However, this method utilizes chemicals or recombinant proteins that are difficult to purify, making industrial application somewhat challenging. The inventors of this invention diligently researched how to maximize the performance of hyaluronic acid and, as a result, discovered that by simultaneously processing hyaluronic acid with enzymes and aging it at low temperatures to produce a hydrolyzed product, it is possible to reduce the molecular weight of the hyaluronic acid and provide excellent irritation-relieving and moisturizing effects. This led to the completion of the present invention. Korean Registered Patent Publication No. 10-2548168Korean Registered Patent Publication No. 10-2013603 Figure 1 is a graph showing the results of cytotoxicity experiments on hyaluronic acid samples produced in Example 1 and Comparative Examples 1-4.Figure 2 is a graph showing the results of the histamine inhibitory activity of the hyaluronic acid samples prepared in Example 1 and Comparative Examples 1 to 4.Figure 3 is a graph showing the results of the NO (Nitric oxide) production inhibitory activity of the hyaluronic acid samples prepared in Example 1 and Comparative Examples 1 to 4.Figure 4 is a graph showing the percentage increase in skin moisture content after treatment with the hyaluronic acid sample prepared in Example 1 and the control sample (Comparative Example 1).Figure 5 is a spectral image showing the molecular weight of the hyaluronic acid hydrolysate from Example 1.Figure 6 is a table summarizing the peak information in the spectrum in which the molecular weight of the hyaluronic acid hydrolysate of Example 1 was measured. Gray cells indicate matrix peak molecular weights. The present invention will be described in detail below. [Cosmetic Composition] This invention provides a cosmetic composition containing an active ingredient. The cosmetic composition may be in the form of, for example, a solution, gel, solid or kneaded anhydrous product, an emulsion obtained by dispersing an oil phase in an aqueous phase, a suspension, a microemulsion, microcapsules, microgranulocytes, or an ionic (liposome) or nonionic vesicle dispersant. More specifically, it can be provided in the form of a lotion, emulsion, cream, skin, serum, essence, emulsion, powder, cosmetic ointment, spray, gel, face pack, cleanser, soap, shampoo, conditioner, bath additive, cleanser, or concealer stick. It may also be manufactured in the form of a foam or an aerosol composit