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JP-7856124-B2 - Microbial culture kit

JP7856124B2JP 7856124 B2JP7856124 B2JP 7856124B2JP-7856124-B2

Inventors

  • 高田 雅親
  • 砂原 博文
  • 青井 議輝

Assignees

  • 株式会社村田製作所

Dates

Publication Date
20260511
Application Date
20240604
Priority Date
20190326

Claims (4)

  1. It comprises three frame structures: an upper, middle, and lower layer, each having an internal space. The three frames described above constitute a three-layer laminated structure that is stacked and connected to one another. The layers are separated by filters that allow for the flow of nutrients/environmental components but prevent the flow of microorganisms. The upper frame allows for the circulation of a nutrient-containing liquid or nutrient-containing gas within its internal space. The intermediate frame is capable of holding a microorganism-containing culture medium in its internal space. The lower frame allows for the circulation of a liquid or gas containing environmental components within its internal space . Each of the three frames has a first fitting portion and a second fitting portion. The first fitting portion and the second fitting portion are mutually fittingable. The three frames mentioned above are either all first-type frames, or a first-type frame and a second-type frame. The first formwork comprises a first frame body surrounding a first internal space, and the first frame body is provided with an inlet passage for introducing fluid into the first internal space and an outlet passage for releasing fluid from the first internal space, both of which are openable and closable. The second formwork comprises a second frame body that surrounds the second internal space, The upper and lower frames are the first formwork, The intermediate frame is either the first formwork or the second formwork. The first mold body arranged in the upper layer is configured such that, when the inlet and outlet passages are open, the nutrient-containing liquid or nutrient-containing gas can be circulated through the first internal space, and when the inlet and outlet passages are closed, the nutrient-containing material can be held in the first internal space. The first mold body arranged in the lower layer is configured such that when the inlet and outlet passages are open, the liquid or gas containing environmental components can be circulated through the first internal space, and when the inlet and outlet passages are closed, the material containing environmental components can be held in the first internal space. It further comprises a base and a lid, The base supports the lower frame so as to close the first internal space of the lower first formwork, The cover closes the upper frame so as to cover the first internal space of the upper first formwork. Microbial culture kit.
  2. The first frame body has an annular shape, The second frame body has an annular shape, The first fitting portion is an annular outer fitting portion having an internal thread, The second fitting portion is an annular inner fitting portion having an external thread, The outer fitting portion of the first frame body can be fitted onto the inner fitting portion of the first frame body and the inner fitting portion of the second frame body by the internal threads being screwed onto the external threads. The inner fitting portion of the first frame body can be fitted into the outer fitting portion of the first frame body and the outer fitting portion of the second frame body by the external screw being screwed into the internal screw, and the outer fitting portion of the second frame body can be fitted into the inner fitting portion of the first frame body and the inner fitting portion of the second frame body by the internal screw being screwed into the external screw, The inner fitting portion of the second frame body can be fitted into the outer fitting portion of the first frame body and the outer fitting portion of the second frame body by the external screw being screwed into the internal screw. The base has an annular inner fitting portion with an external thread at its upper part, and the inner fitting portion is capable of being fitted into the outer fitting portion of the first formwork body arranged in the lower layer. The lid has an annular outer fitting portion with internal threads at its lower part, and the outer fitting portion is externally fitted onto the internal fitting portion of the first mold body arranged in the upper layer. A microbial culture kit according to claim 1 .
  3. The first formwork has pipes extending to the outside from the inlet and/or outlet. A microbial culture kit according to claim 1 .
  4. The first fitting portion and the second fitting portion are, (i) A screwing mechanism using internal and external threads, (ii) Slide fitting mechanism, (iii) uneven fitting mechanism; Constituting at least one of the following: A microbial culture kit according to claim 1.

Description

This invention relates to a microbial culture kit that can constitute a microbial culture apparatus on its own. It is said that the conventional agar plate surface smear method can only cultivate about 1% of the microorganisms in the environment. The reason for this is thought to be as follows: (a) Because the culture environment is closed, excess products from microorganisms cannot be discharged from the system. As a result, microbial metabolites and environmental components accumulate, inhibiting microbial growth. (b) It is difficult to maintain the nutrient concentrations required for the growth of the target microorganism in solid culture media. Therefore, cultivation techniques such as those shown in Patent Documents 1 and 2 have been proposed. Patent Document 1 describes cultivation while continuously supplying liquid culture medium. Patent Document 2 describes cultivation using a solid culture medium containing microorganisms placed in a natural environment. Japanese Patent Publication No. 2016-86654U.S. Patent No. 7011957 This is a perspective view showing a microbial culture kit according to the first embodiment of the present invention.This is a plan view showing the first mold frame of the microbial culture kit.This is a cross-sectional view taken along line III-III in Figure 2.This is a cross-sectional view showing the process of connecting the first formwork units together.This is a partial cross-sectional side view showing the vibrator.This is a view from arrow VI in Figure 5.This is a schematic cross-sectional view showing a microbial culture apparatus configured with the microbial culture kit of the first embodiment.This is a perspective view of the base.This is a view from arrow IX in Figure 8.This is a cross-sectional view taken along line X-X in Figure 9. This is a bottom view of the lid.This is a cross-sectional view taken along line XII-XII in Figure 11.This is a schematic cross-sectional view showing a first modified example of a microbial culture apparatus configured with the microbial culture kit of the first embodiment.This is a schematic cross-sectional view showing a second modified example of a microbial culture apparatus configured with the microbial culture kit of the first embodiment.This is a schematic cross-sectional view showing a third modified example of a microbial culture apparatus configured with the microbial culture kit of the first embodiment.This is a perspective view showing a microbial culture kit according to a second embodiment of the present invention.This is a plan view showing the second type frame of the microbial culture kit.This is a cross-sectional view taken along the line XVIII-XVIII in Figure 17.This is a cross-sectional view showing the process of connecting the first formwork and the second formwork.This is a schematic cross-sectional view showing a first modified example of a microbial culture apparatus configured with the microbial culture kit of the second embodiment. This is a schematic cross-sectional view showing a second modified example of a microbial culture apparatus configured with the microbial culture kit of the second embodiment.This is a schematic cross-sectional view showing a third modified example of a microbial culture apparatus configured with the microbial culture kit of the second embodiment.This is a schematic cross-sectional view showing a fourth modified example of a microbial culture apparatus configured with the microbial culture kit of the second embodiment.This is a perspective view showing an example of a microbial culture kit according to a third embodiment of the present invention.This is a perspective view showing another example of a microbial culture kit according to a third embodiment of the present invention.Figure 24 is a schematic cross-sectional view showing a microbial culture apparatus configured using the microbial culture kit.This is a schematic cross-sectional view showing a first modified example of a microbial culture apparatus configured with the microbial culture kit shown in Figure 24.Figure 25 is a schematic cross-sectional view showing a microbial culture apparatus configured using a microbial culture kit.Figure 25 is a schematic cross-sectional view showing a first modified example of a microbial culture apparatus configured using the microbial culture kit.This is a perspective view showing an example of a microbial culture kit according to the fifth embodiment of the present invention. This is a view taken along the XXXI arrow in Figure 30.This is a view (plan view) from the direction of arrow XXXII in Figure 30.This is a cross-sectional view taken along the line XXXIII-XXXIII in Figure 32.Figure 32 is a perspective view of the cross-section shown.This is a schematic cross-sectional view showing a microbial culture apparatus configured with the microbial culture kit of the fifth embodiment.This is a perspective view showing a modified example of the second formwork.This is a view taken along arro