JP-7856301-B2 - Sulfur compound detector, method for producing the sulfur compound detector, and method for detecting sulfur compounds

JP7856301B2JP 7856301 B2JP7856301 B2JP 7856301B2JP-7856301-B2

Inventors

丸尾容子
加藤諒

Assignees

学校法人東北工業大学

Dates

Publication Date: 20260511
Application Date: 20220608
Priority Date: 20211119

Claims (8)

Detection agent and, A detection agent support material carrying the aforementioned detection agent, Includes, The aforementioned detection agent consists of a disulfide compound having a disulfide and a pyridine ring in its molecule, and sodium acetate . The aforementioned detection agent carrier is made of cellulose fibers. Sulfur compound detector.
The sulfur compound detector according to claim 1 , wherein an electron-withdrawing group is further bonded to the pyridine ring of the disulfide compound.
The sulfur compound detector according to claim 1, wherein the detection agent support material is in the form of a sheet or a flat plate.
The sulfur compound detector according to any one of claims 1 to 3 , wherein the sulfur compound of the sulfur compound detector is a volatile sulfur compound.
A method for producing a sulfur compound detector according to claim 1, The process involves immersing the aforementioned detection agent support material in a detection agent solution, A step of drying the detection agent support material that has been immersed in the detection agent solution, Includes, The detection agent solution comprises the detection agent and a solvent. A method for producing a sulfur compound detector.
The method for producing a sulfur compound detector according to claim 5 , wherein the solvent is ethanol, methanol, or isopropyl alcohol.
An exposure step of exposing a sulfur compound detector according to claim 1 to a gas, A measurement step, in which the amount of light absorbed or reflected by a sulfur compound detector exposed to the gas in the aforementioned exposure step is measured, A detection step in which the presence or absence of sulfur compounds in the gas and/or the sulfur compound content of the gas is detected from the amount of light absorbed or reflected measured in the measurement step, A method for detecting sulfur compounds, including those mentioned above.
An exposure step of exposing a sulfur compound detector according to claim 1 to a gas, The image acquisition step involves acquiring an image of the sulfur compound detector that has been exposed to the gas in the aforementioned exposure step, A detection step is performed to detect, from the image obtained in the image acquisition step, whether or not a sulfur compound is present in the gas, and/or the sulfur compound content of the gas. A method for detecting sulfur compounds, including those mentioned above.

Description

This invention relates to a sulfur compound detector, a method for producing the sulfur compound detector, and a method for detecting sulfur compounds. In particular, it relates to a sulfur compound detector and a detection method for detecting sulfur compounds present in a gas. Periodontal disease is the most common disease affecting people of all ages, from children to the elderly. It is associated with dietary habits, smoking, and other lifestyle-related factors, and is considered one of the diseases associated with lifestyle. Furthermore, it has become clear that periodontal disease and its treatment can influence systemic diseases such as diabetes, coronary artery disease, and aspiration pneumonia. Therefore, appropriate periodontal treatment contributes not only to improving oral health but also to maintaining and promoting overall health. Currently, periodontal disease examinations primarily involve measuring pocket depth, bleeding, X-rays, photographs, and bacterial tests. However, since these examinations are typically performed at dental clinics, regular visits to the dentist for checkups present significant time and financial challenges. Therefore, it was proposed to analyze the concentration of sulfur compounds, known to be the cause of bad breath in people suffering from periodontal disease, in their exhaled breath. Exhaled breath analysis has the advantages of being non-invasive, painless to the subject, and requiring no special skills. Conventional analytical techniques for sulfur compounds in exhaled breath include semiconductor sensors using zinc oxide or cerium oxide (see Patent Documents 1 and 2, and Non-Patent Document 1), biosensors (see Non-Patent Document 2), and quartz crystal oscillator sensors (see Non-Patent Document 3). Methods involving contact between saliva and detection paper containing lead acetate (see Patent Document 3) have also been proposed. For example, Non-Patent Document 1 discloses the existence of a commercially available halitosis meter, Breastron® II, which uses a semiconductor sensor with a comb-shaped electrode made of Pt thin film coated and sintered with cerium oxide, capable of detecting tens of ppb of hydrogen sulfide and methyl mercaptan in 30 seconds. Since this semiconductor sensor is also sensitive to organic compounds such as alcohol, interference from these gases is eliminated by passing the analytical gas through a mouthpiece with an acid-treated silica gel filter. Non-patent document 2 discloses the detection of methyl mercaptan in an aqueous solution using a sensor that utilizes two enzymes: alcohol oxidase and horseradish peroxidase. The enzymes are immobilized on an osmium wire, and the detection of concentrations as low as 0.2 μM is possible by electrochemically detecting the H₂O₂ produced by the enzymatic reaction. Non-patent document 3 discloses a method for detecting micrograms of hydrogen sulfide using a quartz crystal oscillator with a gas permeable membrane and a silver electrode. However, the above-mentioned analytical techniques for sulfur compounds in exhaled breath had the problem of not being able to selectively and easily measure sulfur compounds. For example, in the semiconductor sensor described in Non-Patent Document 1, it was necessary to combine multiple sensors and analyze the output in order to achieve selectivity, and it was also necessary to remove interfering gases through a filter. Furthermore, it was necessary to provide a heater to keep the semiconductor surface at a high temperature during measurement, and it was necessary to keep it powered on at all times in order to extract the signal in real time. Non-patent document 2, which combines a biosensor using an enzyme reaction, has several problems: it requires highly specialized handling, is unstable due to being a biosensor, and the device is large. Non-patent document 3 required precise control of the temperature of the quartz crystal oscillator. Japanese Patent Application Publication No. 01-035368Japanese Patent Publication No. 2004-108861Japanese Patent Publication No. 2004-309283 Kengo Suzuki, Tsuyoshi Ueda, "Measurement of Halitosis Components Using Semiconductor Gas Sensors," Electrochemistry, 2018, 86, 134-137.Z. H. Li, et al., “Design and characterization of methyl mercaptan biosensor using alcohol oxidase”, Sensors & Actuators B, 2014, 192, 680-684F. He, et al., “A Novel QCM-based Biosensor for Detection of Microorganisms Producing Hydrogen Sulfide”, Anal. Lett., 2008, 41, 2697-2709 An example of a method for producing a sulfur compound detector and a method for measuring sulfur compounds according to the present invention is shown.An example of an absorbance spectrum obtained by measuring the absorbance of a gas using the sulfur compound detector of the present invention is shown.An example of an absorbance spectrum obtained by measuring the absorbance of a gas using a comparative example sulfur compound detector is shown.Another example of an absorbance spec