KR-102959893-B1 - Antioxidant and anti-inflammatory composition containing fermented grape skin extract fermented with Lactobacillus platarum and Lactobacillus rhamnosus as an active ingredient

Abstract

The present invention relates to an antioxidant and anti-inflammatory functional composition comprising a lactic acid bacteria fermented product of grape skin extract as an active ingredient, and has significant effects on antioxidant and anti-inflammatory properties.

Inventors

• 임병우
• 정수아
• 정영환

Assignees

• 건국대학교 글로컬산학협력단

Dates

Publication Date

20260507

Application Date

20231124

Priority Date

20221125

Claims (10)

1. In an antioxidant and anti-inflammatory functional cosmetic composition comprising a lactic acid bacteria fermented product of grape skin extract, The above lactic acid bacteria is a composition comprising Lactobacillus plantarum and Lactobacillus rhamnosus mixed in a weight ratio of 1:2.5-3.5.
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5. In paragraph 1, A cosmetic composition in which the above lactic acid bacteria is a mixture of Lactobacillus plantarum and Lactobacillus rhamnosus in a weight ratio of 1:2.8-3.2.
6. In paragraph 1, A cosmetic composition in which the above lactic acid bacteria is a mixture of Lactobacillus plantarum and Lactobacillus rhamnosus in a weight ratio of 1:2.9-3.1.
7. In a composition of a health functional food with antioxidant and anti-inflammatory properties comprising a lactic acid fermented product of grape skin extract, The above lactic acid bacteria is a composition comprising Lactobacillus plantarum and Lactobacillus rhamnosus mixed in a weight ratio of 1:2.5-3.5.
8. In Paragraph 7, A health functional food composition wherein the above lactic acid bacteria is a mixture of Lactobacillus plantarum and Lactobacillus rhamnosus in a weight ratio of 1:2.8-3.2.
9. In a functional health food composition with antioxidant and anti-inflammatory properties comprising a lactic acid fermented product of grape skin extract, The above lactic acid bacteria is a composition comprising Lactobacillus plantarum and Lactobacillus rhamnosus mixed in a weight ratio of 1:2.5-3.5.
10. In Paragraph 9, A health food composition wherein the above lactic acid bacteria is a mixture of Lactobacillus plantarum and Lactobacillus rhamnosus in a weight ratio of 1:2.8-3.2.

Description

Antioxidant and anti-inflammatory composition containing fermented grape skin extract fermented with Lactobacillus platarum and Lactobacillus rhamnosus as an active ingredient The present invention relates to an antioxidant and anti-inflammatory functional composition comprising a lactic acid bacteria fermented product of grape skin extract as an active ingredient. This invention is the result of a project supported by Chungju City Hall. [Department Name] Chungju City Hall [Project Management Agency Name] Chungju City Hall [Research Project Name] Chungju City Bio-industry Growth Foundation Establishment Support Project [Research Project Title] Development of a Functional Material for Blood Sugar Improvement Using Grape Skins, a Specialized Crop of Chungbuk, and Lactic Acid Bacteria Fermented Extracts Containing Them [Name of Project Performing Organization] Human Bioscience Co., Ltd. [Research Period] June 2022 – November 2022 Reactive oxygen species generated during the body's metabolic processes can cause damage to cell membranes, proteins, and DNA, potentially leading to aging and disease. Free radicals and other reactive oxygen species (ROS) can be produced as byproducts during the oxidation process in living organisms (Halliwell & Gutteridge, 1999). While ROS perform many important functions in normal metabolism, excessive ROS can impair protein function, cause oxidative damage to cellular macromolecules, and lead to the death of trigger cells (Kremer et al., 2012). Prolonged conditions of oxidative stress are involved in the development of various diseases, including cardiovascular disease and cancer (Sun et al., 2004). Oxidative damage to DNA, proteins, and other macromolecules is hypothesized to constitute a major type of internal damage leading to aging (Fraga et al., 1990). Therefore, as interest in enjoying a healthy life by consuming antioxidants increases, interest and demand for natural antioxidants that are harmless to the human body are also increasing, leading to the exploration of various antioxidants derived from natural products (Choi et al., 2005). Inflammation occurs when macrophages in the human body respond to external stimuli or invading pathogens by producing cytokines such as TNF-α, interleukin 1-ββ, and IL-6, which are inflammatory mediators. Additionally, they synthesize inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) to produce nitric oxide (NO) and prostaglandin E2 (PGE2). Although NO produced through these processes plays a role in eliminating tumors and bacteria, excessive production of NO in the body causes tissue damage, genetic mutations, and inflammatory responses such as edema by increasing vascular permeability, thereby causing harm to the human body (Moncada et al., 1991; Choi et al., 1993). Consequently, interest in anti-inflammatory substances is increasing. Figure 1 shows the results of measuring the DPPH radical scabengin activity of the grape skin extract and fermented product obtained in Example 1. Figure 2 shows the measurement results of the ABTS radical scavenging ability of the grape skin extract and fermented product obtained in Example 1. Figure 3 shows the results of measuring the DNA oxidation inhibitory effect of the grape skin extract and fermented product obtained in Example 1. Figure 4 shows the results of measuring the intracellular ROS generation rate of the grape skin extract and fermentation extract obtained in Example 1. Figure 5 shows the results of measuring the intracellular ROS generation rate of fermented products obtained by treating grape skin extract with different mixing ratios of L. Plantarum and L. Rhamnosus. The present invention will be described in detail below. Cosmetic composition The present invention provides a cosmetic composition comprising an active substance. The above cosmetic composition may be in the form of, for example, a solution, gel, solid or paste anhydrous product, an emulsion obtained by dispersing an oil phase in an aqueous phase, a suspension, a microemulsion, a microcapsule, a microgranule, or an ionic (liposome) or non-ionic vesicular dispersant. More specifically, it may be provided in the form of a lotion, emulsion, cream, skin toner, lotion, serum, essence, emulsion, powder, cosmetic ointment, spray, gel, pack, cleanser, soap, shampoo, rinse, bath additive, detergent, or concealer stick. Additionally, it may be prepared in the form of a foam or an aerosol composition further containing a compressed propellant. In addition, the cosmetic composition may contain, in addition to the active ingredient of the present invention, auxiliary agents commonly used in the field of cosmetics, such as fatty substances, organic solvents, solvents, thickeners and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or non-ionic emulsifiers, fillers, metal ion chelating agents and chelating agents, preservatives, vitamins, blocker