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KR-102960486-B1 - Anti-bacterial or cell protective composition containing extract of Ligustrum obtusifolium

KR102960486B1KR 102960486 B1KR102960486 B1KR 102960486B1KR-102960486-B1

Abstract

The present invention relates to a composition comprising an extract of Ligustrum obtusifolium as an active ingredient, wherein the Ligustrum obtusifolium extract exhibits an antibacterial effect against Acne Bacteria ( C. acnes ) and Staphylococcus epidermidis ( S. epidermidis ), and also exhibits a cytoprotective effect against cell damage caused by oxidative stress induced by hydrogen peroxide ( H₂O₂ ) in human skin keratinocytes (HaCaT keratinocytes), so it can be used as an antibacterial or cytoprotective composition, specifically as a cosmetic composition, and also as a pharmaceutical composition and a health functional food composition.

Inventors

  • 이남호
  • 김정은
  • 신찬성
  • 김소희

Assignees

  • 제주대학교 산학협력단

Dates

Publication Date
20260507
Application Date
20231228

Claims (6)

  1. A cosmetic composition for skin antibacterial or skin cell protection characterized by comprising an ethanol extract of the leaves or branches of Ligustrum obtusifolium as an active ingredient, The above composition exhibits an antibacterial effect against acne bacteria ( C. acnes ) and Staphylococcus epidermidis , and The above composition is a cosmetic composition for skin antibacterial or skin cell protection , characterized by exhibiting a cytoprotective effect against cell damage caused by oxidative stress induced by hydrogen peroxide ( H₂O₂ ) in human skin keratinocytes (HaCaT keratinocytes).
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  6. A functional food composition for skin antibacterial or skin cell protection characterized by containing an ethanol extract of the leaves or branches of Ligustrum obtusifolium as an active ingredient, The above composition exhibits an antibacterial effect against acne bacteria ( C. acnes ) and Staphylococcus epidermidis , and The above composition is a functional food composition for skin antibacterial or skin cell protection , characterized by exhibiting a cytoprotective effect against cell damage caused by oxidative stress induced by hydrogen peroxide ( H₂O₂ ) in human skin keratinocytes (HaCaT keratinocytes).

Description

Antibacterial or cell protective composition containing extract of Ligustrum obtusifolium The present invention relates to an antibacterial or cell-protective composition comprising an extract of Ligustrum obtusifolium , and more specifically, to a composition characterized by containing an extract of Ligustrum obtusifolium leaves or branches as an active ingredient, which exhibits an antibacterial effect against Acne Bacteria ( C. acnes ) and Staphylococcus epidermidis ( S. epidermidis ) and has a cell-protective effect against cell damage caused by oxidative stress induced by hydrogen peroxide ( H₂O₂ ) in human skin keratinocytes (HaCaT keratinocytes). Acne is a skin condition originating in the hair follicles and sebaceous glands. It occurs when bacteria proliferate and cause inflammation as sebum cannot be expelled due to increased sebum secretion or the narrowing or blockage of pores. Androgens, which begin to be secreted during puberty, promote sebum production and cause hyperkeratosis of the epidermis. Consequently, as hair follicles become blocked, the interior of the follicle becomes an environment where anaerobic bacteria, including Cutibacterium acnes ( C. acnes ), can thrive. Simultaneously, other bacteria such as Staphylococcus epidermidis ( S. epidermidis ) play a role in causing acne and its complications around the hair follicles. C. acnes , the causative agent of acne, is a commensal bacterium present in the skin that is normally tolerated by the skin's immune system. However, skin conditions such as acne develop due to an imbalance in skin lipid composition caused by excessive androgen secretion and the proliferation of C. acnes . S. epidermidis , an aerobic bacterium, grows in the middle of external cysts or hair follicles and is a causative agent that exacerbates primary skin diseases and secondary acne symptoms. Since bacteria such as C. acnes and S. epidermidis play a major role in inducing inflammatory responses, antibiotics are primarily used to treat inflammatory acne. Currently, most medications used for acne treatment are medical antibiotics or chemically synthesized products—such as retinoids, benzoyl peroxide, clindamycin, erythromycin, and triclosan—that are unsuitable for cosmetic application and are known to cause side effects including skin irritation, hepatotoxicity, and the development of resistance. Consequently, there is a rapidly increasing interest in natural antimicrobial substances that are effective for acne-prone skin, safe for the skin, and minimize side effects, and many researchers are striving to develop such acne treatments. Skin aging refers to changes that occur in the skin alongside the aging of the human body. It can be classified into intrinsic aging, which arises from the decline in the function of physiological factors within the skin, and exogenous aging, which is caused by oxidative stress from the external environment. A representative example of exogenous aging is photoaging caused by exposure to sunlight. In skin exposed to sunlight, reactive oxygen species (ROS) are generated through the action of ultraviolet rays. ROS refer to all types of modified oxygen that damage cells; they are produced during oxygen metabolism or generated within the body when continuously exposed to external environmental factors such as ultraviolet rays and stress. Types of ROS include superoxide anion radical (· O₂⁻ ), hydroxyl radical (·OH), and hydrogen peroxide ( H₂O₂ ) . These substances drive damage to skin cells and tissues, destroying the antioxidant defense network composed of antioxidant enzymes and non-enzymatic antioxidants. Consequently, they reduce elasticity and accelerate skin aging by causing wrinkles, age spots, and freckles. Therefore, to prevent skin aging, a system is required that not only inhibits the excessive production of reactive oxygen species but also efficiently removes the generated reactive oxygen species. Synthetic antioxidants are used for this purpose, with butylated hydroxyl toluene (BHT) and butylated hydroxyl anisole (BHA) being representative examples. However, while these synthetic antioxidants exhibit excellent antioxidant activity, they pose safety issues, such as side effects and toxicity, when consumed over a long period; consequently, discussions regarding restrictions on their use have been ongoing. Consequently, there is a demand for the development of antioxidants utilizing physiologically active components found in natural products that have low toxicity and fewer side effects. Accordingly, the inventors completed the present invention by confirming that the extract of the leaves or branches of the Ligustrum plant has excellent antibacterial effects against C. acnes and S. epidermidis , which are causative agents of acne, and has excellent cytoprotective effects against oxidative stress in human skin keratinocytes (HaCaT keratinocytes). The following drawings attached to this specification illustrate preferred embodiments o