KR-102961023-B1 - PLANT VIRUS CONTROL AGENT COMPRISING TURMERIC EXTRACT

Abstract

The present invention relates to a composition for controlling plant viruses comprising an extract of turmeric (Curcumae Longae Rhizoma). The composition of the present invention has high antiviral activity against tobacco mosaic virus.

Inventors

• 김철홍
• 한송희
• 박주연

Assignees

• (주)현농

Dates

Publication Date

20260508

Application Date

20241023

Claims (5)

1. A composition for controlling plant viruses comprising turmeric (Curcumae Longae Rhizoma) extract.
2. In paragraph 1, A composition for controlling plant viruses, wherein the above plant virus is the Tobacco Mosaic Virus (TMV).
3. In paragraph 1, The above turmeric extract is prepared by adding water in an amount of 2 to 4 times the weight of the dried turmeric to the dried turmeric, and A composition for controlling plant viruses, extracted for 0.5 to 2 hours at a temperature of 50 to 70°C.
4. In paragraph 1, The above turmeric extract is prepared by adding ethanol in an amount of 2 to 4 times the weight of the dried turmeric relative to the weight of the dried turmeric, and A composition for controlling plant viruses, extracted at room temperature for 0.5 to 2 hours.
5. In paragraph 1, A composition for controlling plant viruses, wherein the above-mentioned turmeric extract is included in an amount of 0.5 to 15 weight percent relative to the total composition.

Description

Plant virus control agent comprising turmeric extract The present invention relates to a plant disease control agent comprising a turmeric extract, and more specifically, to a plant disease control agent comprising a turmeric extract having a control effect against tobacco mosaic virus. Tobacco Mosaic Virus (TMV) is one of the representative viruses that causes diseases in various plants, including tobacco, and is considered a major problem, particularly in the agricultural sector. Plants infected with TMV exhibit symptoms such as mosaic-patterned spots on the leaves, curling, and discoloration; these symptoms reduce the plant's photosynthetic capacity and can ultimately have a fatal impact on productivity. Globally, the tobacco mosaic virus has long threatened agricultural productivity, and various control methods have been developed. Traditionally, chemical insecticides or pesticides have been used, but their use is limited due to environmental pollution issues and potential harm to the human body. Biological control agents, such as those containing the Pseudomonas chlororaphis O6 strain, are widely studied due to their ability to produce antibiotics and induce immune responses in plants; however, due to the nature of biological control agents, their activity can vary significantly depending on temperature, humidity, and the chemical composition of the soil, and their effectiveness tends to decrease under unsuitable conditions. Furthermore, the methods for culturing, isolating, and storing the strain are more complex compared to other control agents, and the numerous variables involved in these processes may make them difficult to apply in large-scale agricultural systems. Therefore, there is a need for the development of a control agent based on natural substances that can address the tobacco mosaic virus in a safe and efficient manner, while compensating for the shortcomings of chemical and biological strain control agents. Figure 1 is a photograph showing the results of an antiviral activity test of turmeric hot water extract against tobacco mosaic virus. Figure 2 is a graph showing the results of the antiviral activity test of turmeric hot water extract against tobacco mosaic virus. Figure 3 is a photograph showing the results of an antiviral activity test against tobacco mosaic virus according to the extraction method of turmeric extract. Figure 4 is a photograph showing the results of the antiviral activity test against tobacco mosaic virus according to the concentration of turmeric hot water extract. The present invention will be explained in detail below through experimental examples. Preparation Example 1. Preparation of turmeric extract Turmeric extract was prepared to measure the antiviral activity of the turmeric extract against tobacco mosaic virus. First, tobacco seeds were disinfected using 70% ethanol and 2% NaOCl, then thoroughly washed three times with sterile water and sown in sterile bio-soil. After two weeks, the germinated seeds were transplanted into 20 x 20 cm pots and grown for 30 days in a glass greenhouse maintaining a temperature of 26°C and a relative humidity of 30-45%. For hot water extraction, 30g of dried turmeric was soaked in 100ml of distilled water and extracted with hot water at 60℃ for 1 hour. For ethanol extraction, 30g of dried turmeric was extracted with 95% ethanol at room temperature for 1 hour to prepare a 30% (w/v) extract raw material. Experimental Example 1. Results of the virus control activity test of a composition containing turmeric hot water extract The virus control activity of a composition containing a hot water extract of turmeric was measured. The virus inoculum was prepared by adding 2 g of dried diseased tobacco leaves and 0.1 g of diatomite to 10 ml of 0.1 M potassium phosphate (pH 6.8). For the antiviral test, the turmeric extract and the tobacco mosaic virus inoculum were diluted 1:1 in equal volumes to create a composition containing 15%, and then inoculated by making a wound on one side of a tobacco leaf using a cotton swab. The number of lesions occurring on the tobacco leaf was checked on the 5th day after inoculation, and the results are shown in Figures 1 and 2. As a result, the 15% treatment group formed a spot of 65.3±12.6, and Pseudomonas chlororaphis O6 used as a positive control ((+)CON) formed a spot of 18.3±5.0, whereas the untreated group formed a spot of 794.7±26.6. The composition containing turmeric extract showed a high antiviral effect against tobacco mosaic virus, confirming that it exhibited a control efficacy of 74.4% compared to the untreated group. Experimental Example 2. Results of Comparative Test on Virus Control Activity by Extraction Solvent The antiviral activity of turmeric extract against tobacco mosaic virus was measured according to the extraction method. The virus inoculum was prepared by adding 2 g of dried diseased tobacco leaves and 0.1 g of diatomite to 10 ml of 0.1 M potassium phosphate (pH 6.8). For the