KR-102961034-B1 - IMMUNE STRENGTHENING COMPOSITION USING BLACK RADISH EXTRACT

Abstract

The present invention relates to an immune-enhancing composition using a black radish hot water extract and a water-soluble polysaccharide, characterized by containing a black radish hot water extract. The black radish hot water extract is prepared through a black radish drying step in which black radish is washed, cut, and then dried; a hot water extraction step in which distilled water is mixed with and heated the black radish dried through the black radish drying step; a filtration and concentration step in which the black radish extract prepared through the hot water extraction step is filtered and concentrated; a centrifugation step in which ethanol is mixed with and centrifuged the black radish extract concentrated through the filtration and concentration step; and a freeze-drying step in which the black radish extract centrifuged through the centrifugation step is freeze-dried to produce a water-soluble polysaccharide. The black radish hot water extract and water-soluble polysaccharide prepared through the above process exhibit low cytotoxicity and demonstrate an excellent immune-enhancing effect.

Inventors

• 김기옥
• 전형식
• 유경수
• 금은주
• 정영아

Assignees

• 재단법인 제주테크노파크
• 주식회사 유유헬스케어

Dates

Publication Date

20260508

Application Date

20221123

Priority Date

20211125

Claims (12)

1. It contains black radish hot water extract and water-soluble polysaccharides, The above black radish hot water extract is prepared by washing and cutting the black radish, drying it with hot air, then hot water extracting it with distilled water, and filtering and vacuum concentrating the hot water extracted extract. The above-mentioned water-soluble polysaccharide is characterized by being prepared by washing and cutting black radish, hot-air drying, hot-water extraction with distilled water, filtering and vacuum concentrating the hot-water extracted extract, mixing with ethanol, centrifuging, and freeze-drying.
2. The immune-enhancing composition according to claim 1, characterized in that the immune-enhancing composition contains 50 to 200 ug/ml of black radish hot water extract.
3. In claim 1, the immune-enhancing composition is characterized by enhancing immunity through TLR2/4 stimulation.
4. In claim 1, the immune-enhancing composition is characterized by promoting the production of immune-enhancing factors such as nitric oxide (NO), IL-1β, IL-6, and TNF-α.
5. In claim 1, the immune-enhancing composition is characterized by promoting NK cell activation.
6. In claim 1, the immune-enhancing composition is characterized by promoting the production of the immune-modulating factors iNOS and Cox-2.
7. In claim 1, the immune-enhancing composition is characterized by promoting phagocytosis.
8. In claim 1, the immune-enhancing composition is characterized by promoting the generation of reactive oxygen species (ROS).
9. The immune-enhancing composition according to claim 1, characterized in that the immune-enhancing composition promotes the phosphorylation of signaling factors MAPK, NFκB, Akt, and STAT3.
10. In claim 1, the immune-enhancing composition is characterized by inducing macrophage activation through the TLR2/4-MAPK-NFκB-Akt-STAT3 signaling pathway.
11. A health functional food comprising the immune-enhancing composition of claim 1.
12. delete

Description

Immunity-strengthening composition using black radish extract The present invention relates to an immune-enhancing composition using a black radish hot water extract and a water-soluble polysaccharide, and more specifically, to an immune-enhancing composition comprising a black radish hot water extract and a water-soluble polysaccharide that exhibits low cytotoxicity and excellent immune-enhancing effects, which promotes NO production, phagocytosis, cytokine production (IL-1β, IL-6, TNF-α), and activating factors (iNOS, COX-2) through stimulation of TLR (Toll-like receptor) 2/4 of macrophages. Radish ( *Raphanus sativus L.*) is a root plant belonging to the Cruciferae family that has been cultivated for 2,700 years. It possesses 18 chromosomes (2n=2x) and is known to be a self-incompatible plant that produces seeds through pollination by insects; it is also one of the species with very high genetic variation. The origin of the radish is believed to be the Mediterranean coast, and it has now become a commercialized crop, establishing itself as an important vegetable industry in China, Japan, and Korea. Furthermore, genetic variation has led to the creation of various cultivars; in particular, the roots not only possess diverse sizes, colors, and shapes but also exhibit various characteristics (size, shape, surface features, etc.) in their leaves. Representative color characteristics of radishes produced in China include four main types: red, white, green with white, and red fresh. Radishes are an economically important vegetable crop cultivated and consumed globally, particularly in East Asia. Various types and traditional varieties of radishes have been developed through a history of evolution and breeding, exhibiting significant variations in root size, shape, color, taste, and other characteristics such as yield. While the thick roots of radishes are generally harvested as vegetables, there are also varieties used as leafy greens, rapeseed, or canola crops. In Africa, the plant's roots are used as a remedy for many ailments, such as gallbladder problems, diabetes, hepatitis, and gastrointestinal disorders (Chaturvedi 2008). In Korea, it is one of the top ten major vegetables and is consumed in various forms domestically, including kimchi, fried chicken, steamed dishes, seasoned greens, and pickled vegetables. In terms of sales revenue in the domestic vegetable seed market and overseas exports, it ranked third after chili peppers and cabbage (Ministry for food, agriculture, forestry and fisheries, 2012). However, despite being an important vegetable crop in agriculture, genetic and molecular biological research on radishes is lacking compared to other Brassica crops such as Chinese cabbage, cabbage, and canola. Recently, as colored radishes are used in cosmetics, functional foods, and wine, there has been growing interest in the anthocyanin pigments found in radishes. Furthermore, to analyze the genetic relationships of colored radish varieties, genetic analyses such as RAPD (random amplified polymorphic DNA), AFLP (amplified fragment length polymorphism), ITS region analysis, and cpDNA are being actively studied to investigate the phylogenetic relationships between radish varieties. The genus Raphanus originated in the Mediterranean and Black Sea coastal regions (Kaneko et al., 2007). While it is described that R. sativus originated from hybridization between R. maritimus and R. landra (Kitamura 1958, Kaneko et. 2007), most scholars have reported that during the process of spreading from wild radish (R. raphanistrum L.), cultivated radish (R. sativus L.) resulted in the cultivation of varieties such as fresh edible cherry radish (R. sativus L. var. radicola Pers), oil radish (R. sativus L. var. oleifera), fodder radish (R. sativus L. var. caudatus), black radish (R. sativus L. var. niger), and large root radish (Daikon) (R. sativus L. var. longipinnatus Bailey). In Japan, results of analysis using AFLP and SSR markers were reported on wild radish species distributed in East Asia (Huh and Ohnishi, 2002; Ohsako et al., 2010). Additionally, studies on the origin of cultivated radishes in Europe and Asia were reported through the analysis of the kinship between wild and cultivated radishes using mitochondrial sequences (Yamagishi and Terachi, 2003; Yamane, 2004), as well as analysis results using chloroplast sequences (L et al., 2008; Yamane et al., 2005). In Korea, an analysis of the kinship between Korean native and wild species was reported using RAPD markers (Choi et al., 2008), and cultivar classification was reported using AFLP markers (Kwon et al., 2003). Meanwhile, black radish is widely cultivated in regions ranging from the Mediterranean to Asia, Europe, and North America, and is reported to have excellent effects in liver protection, inhibition of lipid metabolism, protection of the intestines and stomach, and anti-inflammatory properties. [Prior Art Literature] [Patent Literature] (Patent Document