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KR-102961184-B1 - Method for extracting low molecular collagen using pollack skin, low molecular collagen, and cosmetic composition having skin regeneration efficacy comprising same

KR102961184B1KR 102961184 B1KR102961184 B1KR 102961184B1KR-102961184-B1

Abstract

The present invention relates to a method for extracting low molecular weight collagen using pollack skin, low molecular weight collagen, and a cosmetic composition having skin regeneration efficacy containing the same.

Inventors

  • 김현수
  • 김유주
  • 송유섭

Assignees

  • 유엔케이코리아 주식회사

Dates

Publication Date
20260508
Application Date
20241115

Claims (10)

  1. A cleaning step of preparing the pollack by separating the skin, washing it once with water, and then washing it a second time under running water; A step of pre-treating the pollack skin by washing the pollack skin with distilled water containing a natural extract; A step of extracting collagen by boiling the above-mentioned pre-treated pollack skin in purified water; and A method for extracting low-molecular-weight collagen comprising the step of reducing the collagen above to a low molecular weight; The step of breaking down the above collagen into low molecular weight molecules a) Acid treatment step to adjust the pH of the collagen to 2 to 4, b) A step of primary low molecular weight reduction by adding a primary acid-active enzyme to acid-treated collagen, c) a step of neutralizing the pH of the primary low-molecular-weight collagen to 7 to 8; and d) A method for extracting low molecular weight collagen comprising the step of adding a secondary neutralizing active enzyme to the neutralized primary low molecular weight collagen to perform secondary low molecular weight conversion, The above primary acidic active enzyme includes pepsin, and The above secondary neutral active enzyme includes pancreatin and nutrasase, and A method for extracting low molecular weight collagen comprising, based on 100 parts by weight of the secondary neutral active enzyme, 0.01 parts by weight or more and 10 parts by weight or less of the pancreatin; and 90 parts by weight or more and 99.99 parts by weight or less of the nutrase.
  2. In claim 1, The above b) further includes a step of adding a primary acidic active enzyme to acid-treated collagen to perform primary low-molecular-weight conversion, and subsequently passing it through an ultrafiltration membrane to increase the content of low-molecular-weight collagen of 3,000 Da or less. A method for extracting low molecular weight collagen, comprising: c) a step of neutralizing the pH of the primary low molecular weight collagen to 7 to 8; and subsequently passing it through an ultrafiltration membrane to increase the content of low molecular weight collagen of 1,000 Da or less.
  3. delete
  4. In claim 1, d) After the step of adding a secondary neutralizing active enzyme to the above-mentioned neutralized primary low-molecular-weight collagen to perform secondary low-molecular-weight conversion, The method further comprises a step of tertiarily reducing the molecular weight of collagen by adding a tertiary neutral active enzyme to the secondary reduced molecular weight collagen; and a step of increasing the content of low molecular weight collagen of 3,000 Da or less by passing it through an ultrafiltration membrane. A method for extracting low molecular weight collagen in which the above-mentioned tertiary neutral active enzyme is collagenase.
  5. In claim 4, A method for extracting low molecular weight collagen, wherein the active temperature in the above first low molecular weight step; the second low molecular weight step and the third low molecular weight step is 30℃ or higher and 40℃ or lower, and the active time is within 12 hours.
  6. In claim 1, The above natural extract includes green tea extract and lemon extract, and A method for extracting low molecular weight collagen, comprising, based on 100 parts by weight of the above natural extract, 20 parts by weight or more and 40 parts by weight or less of the above lemon extract; and 60 parts by weight or more and 80 parts by weight or less of the above green tea extract.
  7. Low molecular weight collagen extracted through a method for extracting low molecular weight collagen according to any one of claims 1, 2 and 4 to 6.
  8. In claim 7, the low molecular weight collagen is a low molecular weight collagen containing 60 wt% or more of collagen with a molecular weight of 1,000 Da or less.
  9. A cosmetic composition having wound healing and skin regeneration efficacy comprising low molecular weight collagen according to claim 7; and an amino acid including proline.
  10. In claim 9, A cosmetic composition having wound healing and skin regeneration efficacy, comprising, based on 100 parts by weight of the total cosmetic composition, 1 part by weight or more and 5 parts by weight or less of the low molecular weight collagen, and 10 parts by weight or more and 15 parts by weight or less of the amino acid.

Description

Method for extracting low molecular collagen using pollack skin, low molecular collagen, and cosmetic composition having skin regeneration efficacy comprising same The present invention relates to a method for extracting low molecular weight collagen using pollack skin, low molecular weight collagen, and a cosmetic composition having skin regeneration efficacy containing the same. As environmental pollution resulting from modern industrialization, as well as fine dust and ultraviolet rays in the atmosphere, have undesirable effects on the skin of modern people, leading to skin roughness and accelerated skin aging, there is a growing demand for preventing dryness and skin roughness by blocking moisture evaporation and maintaining skin moisture for a long time, as well as for improving wrinkles caused by photoaging. The skin is the outermost organ of the human body and serves as a barrier protecting our body from harmful external substances such as microorganisms, irritants, and pollutants. The skin is composed of the epidermis, dermis, and subcutaneous fat layer. The epidermis is divided into the stratum corneum, stratum lucidum, stratum granulosum, stratum spinosum, and stratum basale according to the keratinization process. Most of the cells composing the epidermis are keratinocytes, which form the skin barrier by synthesizing proteins such as transglutaminase-1 and filaggrin, and also participate in skin moisturization by synthesizing hyaluronic acid synthase (HAS), which produces hyaluronic acid, and aquaporin-3, which is involved in the movement of water and glycerol (Jiwon H, et al., 2021). Therefore, strengthening the skin barrier can be considered an essential requirement for skin health. The skin is broadly divided into three layers in order from the outside: the epidermis, dermis, and subcutaneous adipose tissue. It functions to protect the human body from physical and chemical stimuli from the external environment. In particular, the skin regulates the evaporation of approximately 65–70% of the body's water. Among these, the epidermis is divided from the outside in the order of the stratum corneum, stratum granulosum, stratum spinosum, and stratum basale. The stratum corneum of the epidermis contains about 10–20% water and is located at the outermost layer of the body, inhibiting water evaporation from the body while blocking the excessive penetration of substances from the outside (J. Invest. Dermatol. 80(Suppl.), 44-49. 1983). The cells constituting this stratum corneum contain high concentrations of Natural Moisturizing Factor (NMF), a water-soluble component, which not only helps the skin exhibit flexibility but also helps maintain adequate moisture (J. Invest. Dermatol. 54, 24-31, 1970). However, due to causes such as environmental changes, changes in lifestyle patterns, various stresses and environmental pollution arising from social life, frequent washing due to makeup habits, and natural skin aging due to increasing age, the moisture in the stratum corneum decreases, causing the skin to become dry, rough on the surface, and lose its luster, appearing dull. Consequently, the importance of skin moisturization is growing. Recently, among the factors for skin health, natural moisturization, anti-aging, skin regeneration, and elasticity improvement are important, along with strengthening the skin barrier. In particular, collagen, which is essential for skin regeneration and elasticity improvement, is an abundant fibrous structural protein of animal origin that accounts for 30% of the total protein in vertebrates and invertebrates. It forms a triple helix structure and plays a role in forming most of the organic matter in the skin, bones, and teeth in various forms, with particularly high content in bones and skin (dermis). The basic unit of collagen is troprocollagen, which possesses physical or biological stability through intramolecular or intermolecular covalent cross-linking. While the amino acid composition of collagen varies slightly depending on the type, glycine typically accounts for about one-third of the total, proline for one-fourth, and hydroxyproline for one-seventh. Hydroxyproline, an amino acid constituting collagen, forms a specific ratio (12.5–14%) along with hydroxylysine, serving as an indicator component for quantifying collagen; it is known that the composition ratio of collagen varies depending on the species and age of the organism. Regarding research on collagen derived from marine products, many studies have been reported on collagen extracted from the connective tissues and skin of vertebrates and invertebrates such as starfish, squid, jellyfish, and skates. Various studies on these fish collagens, including extraction conditions and physicochemical properties, have been reported using species such as the Japanese sea bream ( Lutjanus lutjanus ) (Kittiphattanabawon et al. 2005), sea bass ( Lates niloticus ) (Muyonga et al. 2004), yellowfin tuna ( Thunnus albacares ) (