KR-102961451-B1 - Novel strain having excellent polyhydroxyalkanoates productivity under high salt concentration and uses thereof

Abstract

The present invention relates to a novel Halomonas litopenaei strain with excellent polyhydroxyalkanoate production under high salt conditions and its use. Among the Halomonas strains, which differ in whether polyhydroxyalkanoates are produced depending on the species, a novel Halomonas litopenaei YBW-3-4-1 strain was selected that produces polyhydroxyalkanoates at significantly high content and concentration under high salt conditions, and thus it can be utilized for the production of polyhydroxyalkanoates.

Inventors

• 김성보
• 이상재
• 손유진
• 신혜진
• 간밧 손도르
• 박미화
• 간밧 다리마

Assignees

• 연세대학교 산학협력단

Dates

Publication Date

20260511

Application Date

20250730

Priority Date

20241022

Claims (13)

1. Halomonas litopenaei strain YBW-3-4-1 deposited under accession number KCTC19194P.
2. In claim 1, a strain having the 16s rRNA of SEQ ID NO. 1.
3. A strain according to claim 1, having salt tolerance at a salinity of 1 to 20% (w/v).
4. In claim 1, having the ability to produce polyhydroxyalkanoates (PHA), The above polyhydroxyalkanoate is a strain of poly(3-hydroxybutyrate).
5. delete
6. A strain according to claim 1, wherein polyhydroxyalkanoate accumulates in the cell at a content of 50 to 65% under salinity conditions of 10 to 20% (w/v).
7. A strain according to claim 1 that produces polyhydroxyalkanoate at a concentration of 1 to 8.5 g/L under salinity conditions of 10 to 20% (w/v).
8. delete
9. A composition for producing polyhydroxyalkanoate comprising the strain of claim 1, The above-mentioned polyhydroxyalkanoate is a composition in which poly(3-hydroxybutyrate) is used.
10. A method for producing polyhydroxyalkanoate comprising the step of culturing the strain of claim 1, The above polyhydroxyalkanoate is poly(3-hydroxybutyrate).
11. A method for producing polyhydroxyalkanoate according to claim 10, wherein the polyhydroxyalkanoate is cultured in a medium containing 10 to 20% (w/v) of NaCl.
12. A method for producing polyhydroxyalkanoate according to claim 10, wherein the method involves culturing in a medium containing 1 to 5% (w/v) of a carbon source.
13. A method for producing a polyhydroxyalkanoate according to claim 12, wherein the polyhydroxyalkanoate is cultured in a medium comprising a carbon source comprising one or more selected from the group consisting of glucose, sucrose, glycerol, fructose, galactose, and xylose.

Description

Novel strain having excellent polyhydroxyalkanoates productivity under high salt concentration and uses thereof The present invention relates to a novel Halomonas lithophena A strain having excellent polyhydroxyalkanoate productivity under high salt conditions and the use thereof. Plastics are polymer materials that have dramatically advanced the convenience of human life. Due to their lightweight nature, moldability, processability, cost-effectiveness, and excellent durability, they are used for a wide range of purposes, from industrial materials to disposable consumables. However, most plastics used universally—such as those for industrial packaging, food packaging, household use, and agriculture/horticulture—exist semi-permanently without decomposing in the natural environment. Consequently, environmental pollution issues arising from the disposal of discarded plastics have persisted. As an alternative to address this, research on biodegradable plastics is actively underway. Biodegradable plastics are plastics that can be broken down by bacteria or living organisms. Because they decompose without leaving behind pollutants, they are receiving significant attention in the field of biotechnology, as they are expected to potentially contribute to reducing solid waste, such as marine microplastics and carbon dioxide emissions from petroleum-based plastics. Bio-based, biodegradable plastics are referred to as biodegradable bioplastics, with representative examples including polylactic acid (PLA) and polyhydroxyalkanoates (PHA). When landfilled, biodegradable bioplastics can decompose into water and carbon dioxide within 6 months to 5 years. Currently, bioplastics account for approximately 1% (2 million tons) of the over 360 million tons of plastic produced annually, with about 1.2 million tons of this being biodegradable plastics. However, this figure is expected to increase to 1.8 million tons by 2025, with the market growth rate for polyhydroxyalkanoates projected to increase tenfold. As demand rises and the availability of high-performance biopolymers and products increases, the bioplastics market is expected to continue growing and diversifying. Polyhydroxyalkanoates (PHAs) are intracellular energy storage compounds produced by various microorganisms under limited nutrient conditions and are biodegradable polymers composed of various types of hydroxycarboxylic acids. Polyhydroxyalkanoates have properties similar to existing synthetic polymers derived from petroleum, such as polybutylene adipate terephthalate (PBAT), polybutylene succinate (PBS), polybutylene succinate terephthalate (PBST), and polybutylene succinate adipate (PBSA), while exhibiting complete biodegradability and excellent biocompatibility. In addition, since mechanical properties and melting points can be controlled by adjusting the type and ratio of polyhydroxyalkanoate monomers, it is being used as an alternative to petroleum plastics in various industrial fields such as medicine, food, and energy. Figure 1 is a figure confirming the intracellular PHA accumulation of Halomonas xianhensis HN-1-3-2, Cobetia marina HN-1-7-2, Halomonas ventosae M434, Halomonas litopenaei YBW-3-4-1, and Halomonas xianhensis YBW-3-4-2 strains by TEM analysis. Figure 2 is a figure analyzing the growth ability of halophilic strains according to salinity. Figure 3 is a diagram showing the analysis of the whole genome of Halomonas litopenaei YBW-3-4-1. Hereinafter, the present invention will be described in detail with reference to the attached drawings and embodiments thereof. However, the following embodiments are presented as examples of the present invention, and if it is determined that a detailed description of a technology or configuration well known to a person skilled in the art may unnecessarily obscure the essence of the present invention, such detailed description may be omitted, and the present invention is not limited thereby. The present invention is capable of various modifications and applications within the scope of the claims set forth below and the equivalent scope interpreted therefrom. Furthermore, the terminology used in this specification is used to appropriately describe preferred embodiments of the present invention, and may vary depending on the intent of the user or operator, or the conventions of the field to which the present invention belongs. Accordingly, the definitions of these terms should be based on the content throughout this specification. Throughout the specification, when a part is described as "comprising" a certain component, unless specifically stated otherwise, this means that it does not exclude other components but may include additional components. All technical terms used in this invention, unless otherwise defined, are used in the sense generally understood by a person skilled in the art in the relevant field of this invention. Furthermore, while preferred methods or samples are described herein, similar or equivale