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KR-102961592-B1 - Novel Priestia megaterium strain having malodor reducing activity and use thereof

KR102961592B1KR 102961592 B1KR102961592 B1KR 102961592B1KR-102961592-B1

Abstract

The present invention relates to a novel strain of Pristia megaterium having odor-reducing activity, a feed composition containing the same, and a method for reducing odors in livestock barns using the same. Since it can reduce odors generated during animal rearing, it is industrially advantageous as it can improve the working environment of the barn, increase work efficiency, and reduce complaints from neighbors. Furthermore, it can increase the productivity of livestock barns by lowering the disease and mortality rates of animals.

Inventors

  • 이병열

Assignees

  • (주)지넥스바이오

Dates

Publication Date
20260512
Application Date
20221031

Claims (13)

  1. Priestia megaterium BTN4 KCTC 15147BP, containing the gdhA gene and possessing odor-reducing efficacy, The above-mentioned Priestia megaterium BTN-4 strain is characterized by having acid resistance and bile resistance, Priestia megaterium BTN4 KCTC 15147BP.
  2. delete
  3. A feed additive comprising Priestia megaterium BTN4 KCTC 15147BP or a culture solution thereof according to claim 1.
  4. In paragraph 3, The above feed additive is characterized by further including yucca extract and an amino acid mineral complex.
  5. In paragraph 4, A feed additive characterized in that the above yucca extract is a 50% (v/v) ethanol extract.
  6. In paragraph 4, A feed additive characterized in that the above amino acid mineral complex is zinc aspartate hydrate represented by the following chemical formula 1. [Chemical Formula 1]
  7. In paragraph 4, A feed additive characterized by the above-mentioned yucca extract, amino acid mineral complex, and Priestia megaterium BTN4 KCTC 15147BP or a culture solution thereof being mixed in a weight ratio of 1:1:1.
  8. In paragraph 3, The above feed additive is a feed additive characterized by reducing odors generated during animal feeding activities.
  9. In paragraph 8, A feed additive characterized in that the above-mentioned odor-causing substance is ammonia.
  10. In paragraph 3, The above feed additive is a feed additive characterized by having an immune-enhancing effect on animals.
  11. A feed composition comprising a feed additive according to paragraph 3.
  12. A method for reducing odors in livestock rearing, comprising the step of adding a feed additive according to paragraph 3 to the livestock feed and feeding it.
  13. In Paragraph 12, The above method is characterized by reducing the amount of ammonia generated from the feeding activities of livestock.

Description

Novel Priestia megaterium strain having malodor reducing activity and use thereof The present invention relates to a novel Pristia megaterium strain having odor-reducing activity, a feed composition containing the same, and a method for reducing odors in livestock barns using the same. The pig farming industry accounts for the largest share (15.2%) of agricultural production value, and its proportion is increasing every year. The amount of domestic pig manure generated has been increasing by an average of 2.23% annually from 2013 to 2019. Odors emanating from livestock barns have a serious impact on the living environment of residents living near farms, and the discomfort index regarding manure odor varied by livestock type, with pigs showing the highest level. Odors from livestock barns not only increase health issues for livestock farmers, such as reduced lung function, bronchitis, nasal mucosal disease, esophagitis, and headaches, but also significantly affect the health and productivity of livestock; consequently, complaints regarding odors are on the rise. To resolve public complaints, the government is making significant efforts to reduce livestock odors by allocating and operating budgets for measures such as spraying microorganisms inside livestock barns, installing misting facilities, distributing deodorizers, spraying deodorizers around barns, installing shielding facilities at composting plants, and dredging Joeuncheon Stream to prevent manure from flowing into the river. Nevertheless, because feed containing excess nutrients is still being fed to increase livestock productivity, the trend is continuing due to odor components (sulfur compounds, volatile fatty acids, phenols and indoles, ammonia and volatile amines) remaining in the digestive organs and manure. To address the odor problem, conventional methods such as biofilters using microorganisms, activated carbon adsorption, and plasma decomposition have been proposed; however, these methods have limitations in continuously and sustainably removing high concentrations of odors and entail enormous costs, making a fundamental solution urgently needed. Accordingly, the inventors have completed the present invention by identifying a novel microorganism capable of effectively removing high concentrations of odors and enhancing disease defense capabilities through immune enhancement in pigs for sustainable livestock farming. Figure 1 is the result of molecular phylogenetic analysis based on the nucleotide sequence of the 16S rRNA gene of BTN1 (sequence number 1), and Figure 2 is the result of molecular phylogenetic analysis based on the nucleotide sequence of the 16S rRNA gene of BTN4 (sequence number 2). Figure 3 shows the results of confirming whether BTN1 and BTN4 strains possess the gdhA gene. The present invention will be described in more detail below. One aspect of the present invention relates to Priestia megaterium BTN4 KCTC 15147BP having the efficacy of reducing odors in livestock barns. The above Priestia megaterium BTN4 KCTC 15147BP or a culture thereof may also be used as a composition for odor reduction. The above-mentioned Priestia megaterium BTN4 strain is a strain deposited under accession number KCTC 15147BP. The above-mentioned Priestia megaterium BTN4 strain was deposited at the Korea Culture Collection Center (KCTC) of the Korea Research Institute of Biotechnology and Bioengineering on October 20, 2022, under accession number KCTC 15147BP. Accordingly, persons skilled in the art can freely obtain and easily acquire the strain, and can verify the characteristics and effects of the microorganism. The above-mentioned Priestia megaterium BTN4 strain may be a strain capable of inhabiting the intestines of animals, including humans, and may have various effects on the health of humans or animals other than humans while inhabiting the intestines. Common microorganisms of the genus Priestia are Gram-positive rod-shaped bacteria belonging to the family Bacillaceae of the order Bacillales, and have previously been known as Bacillus megaterium. The above-mentioned Priestia megaterium BTN4 strain may be a safe microorganism as it does not exhibit toxicity or cause disease in animals, including humans, and may function as a probiotic microorganism, acting as a beneficial bacterium that helps the health of humans or animals other than humans in the intestines. The Priestia megaterium BTN4 strain according to the present invention may have acid resistance and bile resistance. The "acid resistance" refers to the property of withstanding acidic conditions well, and may be a property in which fewer bacteria die under acidic conditions compared to conventional strains, or a property in which the bacteria can proliferate even under acidic conditions. The acidic conditions may be pH 1 to pH 4, and for example, the Priestia megaterium BTN4 strain may withstand acidic conditions caused by animal stomach acid well. The above "bile tolerance" refers to