KR-102961749-B1 - Streptomyces bacillaris S8 strain increasing environmental stress resistance of plant and having antifungal activity against plant pathogen and uses thereof

Abstract

The present invention relates to a Streptomyces bacillus S8 strain that enhances the environmental stress tolerance of plants and has antifungal activity against plant pathogens, and to the use thereof. The Streptomyces bacillus S8 strain of the present invention has excellent antifungal activity against the causative agents of anthracnose, root rot, blight, and brown spread, and has an excellent effect of increasing the cold stress tolerance of turfgrass.

Inventors

• 곽연식

Assignees

• 경상국립대학교산학협력단

Dates

Publication Date

20260507

Application Date

20230531

Claims (10)

1. Streptomyces bacillaris S8 strain with accession number KCTC19090P, which enhances cold stress tolerance in plants and has antifungal activity against Colletotrichum truncatum , Fusarium sp., Phytophthora sojae , and Rhizoctonia solani .
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4. A composition for controlling plant diseases comprising the strain of claim 1 or a culture solution thereof as an active ingredient, A composition for controlling plant diseases, characterized in that the above plant diseases are one or more selected from the group consisting of anthracnose caused by Colletotrichum truncatum , Pythium root rot caused by strains of the genus Fusarium sp., Phytophthora root rot caused by Phytophthora sojae , and Large patch caused by Rhizoctonia solani .
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6. A method for preparing a composition for controlling plant diseases comprising the step of culturing the strain of claim 1, A method for preparing a composition for controlling plant diseases, characterized in that the above-mentioned plant disease is one or more selected from the group consisting of anthracnose caused by Coletotrichum truncatum, root rot caused by strains of the genus Fusarium, blight caused by Phytophthora material, and brown spread disease caused by Rhizoctonia solani.
7. A method for controlling plant diseases comprising the step of applying an effective amount of the composition for controlling plant diseases of claim 4 to a plant, plant seeds, or plant planting soil, wherein A method for controlling plant diseases characterized by the above plant diseases being one or more selected from the group consisting of anthracnose caused by Coletotrichum truncatum, root rot caused by Fusarium strains, late blight caused by Phytophthora material, and brown spread disease caused by Rhizoctonia solani.
8. A composition for enhancing low-temperature stress tolerance in plants, comprising the strain of claim 1 or a culture solution thereof as an active ingredient.
9. A method for enhancing low-temperature stress tolerance of plants, comprising the step of treating an effective amount of the composition of claim 8 to a plant, plant seeds, or plant planting soil.
10. A method for preparing a composition for enhancing low-temperature stress tolerance of plants, comprising the step of culturing the strain of claim 1.

Description

Streptomyces bacillaris S8 strain increasing environmental stress resistance of plant and having antifungal activity against plant pathogens and uses thereof The present invention relates to a Streptomyces bacillus S8 strain that enhances the plant's resistance to environmental stress and has antifungal activity against plant pathogens, and to the use thereof. Modern agriculture relies on the use of synthetic pesticides to protect crops from disease; however, agriculture utilizing these chemical pesticides causes numerous adverse effects on humans and the environment, including environmental pollution, high toxicity, the emergence of pesticide-resistant pathogens, and pesticide residues in the soil and agricultural products. Amid concerns over these side effects, there is a growing preference for eco-friendly farming methods, driven by a recent increase in awareness regarding environmental protection and the safety of agricultural products. Currently, active research is being conducted on the use of microorganisms as well as by-products such as rice bran, food waste, and crab shells for eco-friendly agricultural production. Among these, farming methods utilizing antagonistic microorganisms, which exhibit excellent disease control capabilities in crops and outstanding effects in promoting crop growth and improving soil quality, are becoming widely adopted. Entering the 21st century, OECD countries, including Korea, have been actively promoting eco-friendly agricultural policies to reduce the use of chemical pesticides and fertilizers due to side effects such as environmental pollution and pesticide residues. In Korea as well, interest in biological pest control methods using microbial agents is rising as the advantages of biopesticides—such as the absence of concerns regarding residual toxicity in agricultural products and the non-disruption of agricultural ecosystems—become known. Domestic research on biopesticides began in 1980 with the development of Vicona for controlling ginseng root rot in 1987. Since then, research institutes such as the Korea Research Institute of Chemical Technology, the Korea Research Institute of Bioscience and Biotechnology, and the Agricultural Science and Technology Institute, as well as universities and companies nationwide, have been striving to develop new microbial pesticides that are safe for humans and the environment. However, microorganisms with antagonistic capabilities selected under limited laboratory conditions often fail to colonize and survive in actual fields, and there are very many cases where they fail to exhibit antagonistic effects because antagonistic bacteria are selected only under restricted laboratory conditions. Furthermore, while research on plant disease control using antagonistic microorganisms in crop cultivation is being promoted primarily by universities, national agricultural research institutes, and government-funded research institutes, domestic research personnel and accumulated technology are not yet as sufficient as in foreign countries. Consequently, the use and commercialization of agricultural biopesticides effective for crop production under domestic cultivation conditions remain minimal. Furthermore, plants are subjected to various environmental stresses—such as high temperatures, salinity, drought, pollution, injury, cold damage, excessive light conditions, ozone, excessive UV exposure, and osmotic shock—as well as biological stress caused by pathogens, pests, and viruses, which arise from the deterioration of the global environment. These environmental stresses are one of the factors that severely limit crop productivity and constitute a difficult challenge in the field of agriculture. Therefore, there is a demand for new and groundbreaking technologies to induce resistance to environmental stresses, such as salinity, heat tolerance, drought tolerance, and osmotic shock, in major plants and crops. Meanwhile, Korean Published Patent No. 2021-0117595 discloses 'a microbial preparation for enhancing environmental stress tolerance in plants containing a strain of the genus Flavobacterium TCH3-2 as an active ingredient and its use,' and Korean Registered Patent No. 2248558 discloses 'a strain of Paenibacillus polymyxa JE201 having antifungal activity against plant pathogenic fungi and its use,' but there is no description of the present invention's 'streptomyces bacilli S8 strain enhancing environmental stress tolerance in plants and having antifungal activity against plant pathogens and its use.' Figure 1 is a genome map showing the chromosome and plasmid of the Streptomyces bacillaris S8 strain of the present invention. Figure 2 shows the results of confirming the antifungal activity of the Streptomyces bacilli S8 strain of the present invention against Collectotrichum truncatum , the causative agent of soybean anthracnose; Fusarium sp., the causative agent of root rot in underground soybeans; and Phytophthora sojae , the causative agen