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KR-102961865-B1 - COLORIMETRIC SENSOR FOR DETECTING BACTERIA AND/OR VIRUSES

KR102961865B1KR 102961865 B1KR102961865 B1KR 102961865B1KR-102961865-B1

Abstract

As a colorimetric sensor (1) for detecting bacteria and/or viruses, this colorimetric sensor - One or more layers having a photonic crystal structure (3', 3", ...); - A functional layer (4) that overlaps with one or more layers (3', 3", ...) having a photonic crystal structure, comprising a nanomaterial capable of generating bacterial bioreactive and/or viral bioreactive surface plasmons, and - The bacterial bioreactive and/or viral bioreactive nanomaterial of the functional layer (4) is doped with a protein material or antibody that acts as a viral receptor, or the colorimetric sensor (1) comprises a receptor layer (5) containing a protein material or antibody that acts as a viral receptor, and the functional layer (4) and the receptor layer (5) overlap each other; and/or - The above colorimetric sensor (1) comprises a plasmonic nanostructured layer (7), the plasmonic nanostructured layer comprises an etched nanostructure such as to produce a plasmonic color, and overlaps with layers (3', 3", …) having a photonic crystal structure.

Inventors

  • 라디세 디노

Assignees

  • 디쥐 그룹 에스.피.에이.

Dates

Publication Date
20260507
Application Date
20210329
Priority Date
20200504

Claims (19)

  1. As a colorimetric sensor (1) for detecting bacteria and/or viruses, - A functional layer (4) having a thickness between 4 nanometers and 20 nanometers, comprising a nanomaterial capable of generating surface plasmons and being bacterial bioreactive and/or viral bioreactive, wherein the nanomaterial comprises silver or a silver-based material, or gold, or a gold-based material; - A receptor layer (5) comprising a protein substance or antibody that acts as a receptor for bacteria or viruses, wherein the functional layer (4) and the receptor layer (5) overlap and are in direct contact with each other; - It includes a plasmonic nanostructured layer (7) that is distinct from the functional layer (4) and includes etched nanostructures that produce a plasmonic color, and The functional layer (4) overlaps with the plasmonic nanostructured layer (7), and The etched nanostructures of the plasmonic nanostructured layer (7) are etched into a metal layer or a polymer layer coated with metal nanoparticles, Colorimetric sensor.
  2. In claim 1, the protein substance or antibody acting as a virus receptor comprises a colorimetric sensor (1) containing an ACE2 protein (angiotensin-converting enzyme 2).
  3. A colorimetric sensor (1) further comprising a second functional layer (6) which is a nanomaterial capable of generating surface plasmons and is bacterial bioreactive and/or viral bioreactive, and which overlaps with the receptor layer (5) and is on the opposite side of the functional layer (4).
  4. In paragraph 3, the second functional layer (6) comprises the same nanomaterial as the nanomaterial of the functional layer (4), in a colorimetric sensor (1).
  5. In paragraph 3, a colorimetric sensor (1) in which the second functional layer (6) and the receptor layer (5) are in direct contact with each other.
  6. In paragraph 3, the second functional layer (6) has a thickness between 4 nanometers and 20 nanometers, and is a colorimetric sensor (1).
  7. In claim 1, the etched nanostructure of the plasmon nanostructured layer (7) is formed in a shape that causes surface plasmon resonance, the colorimetric sensor (1).
  8. A colorimetric sensor (1) according to claim 1, wherein the diffraction order of the etched nanostructure of the plasmonic nanostructure layer (7) is 0.
  9. In claim 1, the etched nanostructure of the plasmonic nanostructured layer (7) is configured to produce a polarizing optical effect, the colorimetric sensor (1).
  10. A colorimetric sensor (1) further comprising a support layer (2) in claim 1.
  11. In paragraph 1, a colorimetric sensor (1) implemented in the form of a label.
  12. In paragraph 1, a colorimetric sensor (1) applied to a device (200), a sheet, or a roll-type support.
  13. In claim 1, the functional layer (4) is optionally arranged to form an alphanumeric string, or an image, or a symbol, or a code, of a colorimetric sensor (1).
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Description

Colorimetric sensor for detecting bacteria and/or viruses The present invention relates to a colorimetric sensor for detecting bacteria and/or viruses. Colorimetric sensors are known for detecting bacterial contaminating pathogens, such as Escherichia coli. Examples of colorimetric sensors for detecting bacteria, such as E. coli, are described in the literature [G.M. Paterno, L. Moscardi, S. Donini, D. Ariodanti, I. Kriegel, M. Zani, E. Parisini, F. Scotognella, G. Laznani, "Hybrid One-Dimensional Plasmonic Photonic Crystals for Optical Detection of Bacteria Contaminants", J. Phys. Chem. Lett. 2019, 10, 4980-4986]. These sensors comprise a silver layer (plasmonic metal) and a one-dimensional photonic crystal. Silver exhibits bioresponsivity to E. coli bacteria, changing the photonic response upon contact with E. coli bacteria. That is, for example, when the presence of bacteria is detected by contacting a subject's secretion with the silver layer, a change in the sensor's color is detected. However, to detect other contaminating pathogens or viruses that are generally much smaller than bacteria, a sensor with higher sensitivity is required. Accordingly, the object of the present invention is to provide a colorimetric sensor having enhanced sensitivity that enables accurate detection of bacteria and viruses, such as the COVID-19 virus. This purpose and other purposes are satisfied by a colorimetric sensor for detecting bacteria and/or viruses according to claim 1 and a colorimetric sensor for detecting bacteria and/or viruses according to claim 18. Dependent claims limit possible preferred embodiments of the present invention. To ensure a good understanding of the invention and to recognize its advantages, some non-limiting exemplary embodiments will be described below with reference to the accompanying drawings. FIG. 1 is a schematic cross-sectional view of a colorimetric sensor according to a possible first embodiment of the present invention. FIG. 2 is a schematic cross-sectional view of a colorimetric sensor according to a possible second embodiment of the present invention. FIG. 3 is a schematic cross-sectional view of a colorimetric sensor according to a possible third embodiment of the present invention. FIG. 4 is a schematic cross-sectional view of a colorimetric sensor according to a possible fourth embodiment of the present invention. FIG. 5 is a schematic cross-sectional view of a colorimetric sensor according to a possible fifth embodiment of the present invention. FIG. 6 is a schematic cross-sectional view of a colorimetric sensor according to a possible sixth embodiment of the present invention. Figure 7 is a schematic cross-sectional view of a one-dimensional photonic crystal. FIGS. 8a to 8c are perspective views of a kit including a colorimetric sensor according to a possible embodiment of the present invention in different usage states. FIGS. 9a to 9c are perspective views of a kit including a colorimetric sensor according to a possible embodiment of the present invention in different usage states. FIGS. 10a to 10c are perspective views of a kit including a colorimetric sensor according to a possible embodiment of the present invention in different usage states. FIGS. 11 to 14 are schematic cross-sectional views of a colorimetric sensor according to other possible embodiments of the present invention. Referring to the attached FIGS. 1 through 6, a colorimetric sensor for detecting bacteria and/or viruses is generally denoted by reference numeral 1. The sensor (1) may be made, for example, as a label applied to one or more sheets or roll-type supports, or as a label applied to an apparatus (200), for example, as illustrated in FIGS. 8 through 10. As an example, the sensor (1) implemented in the form of a label may be immersed directly or indirectly by the withdrawing device (201) of the apparatus (200) in a container that receives a sample of the secretion to be analyzed from a subject. Alternatively, the sensor (1) implemented in the form of a label may be applied directly or indirectly by the withdrawing device (201) of the apparatus (200) to a subject, for example, the tongue, to be tested for infection. For example, referring to FIGS. 8a to 8c, the mechanism (200) may include a body (202) formed in the shape of a spatula to which the sensor (1) is applied. Referring to FIGS. 9a through 9c, the apparatus (200) may include a spatula-shaped body (202) to which a sensor (1) is applied, and a second spatula-shaped body (203) to which a sampling device (201) is applied. When the second spatula-shaped body (203) is rotated relative to the spatula-shaped body (202), the sampling device (201) comes into contact with the sensor (1). According to the illustrated embodiment, the relative rotation of the spatula-shaped bodies is centered on an axis perpendicular to the longitudinal axis of the spatula-shaped bodies aligned with each other. Referring to FIGS. 10a through 10c, the apparatus (200) may in