Search

KR-102961866-B1 - Viridibacillus sp. JNUCC6 strain having skin whitening and anti-inflammatory activity and uses thereof

KR102961866B1KR 102961866 B1KR102961866 B1KR 102961866B1KR-102961866-B1

Abstract

The present invention relates to a strain of the genus Viridibacillus JNUCC6 having skin whitening and anti-inflammatory activity and other uses. It is expected that the strain, its lysate, culture medium, extract of the culture medium, and fermentation medium can be used in cosmetics, pharmaceuticals, health foods, etc., as it has been confirmed that they inhibit melanin production and tyrosinase activity and reduce nitric oxide production.

Inventors

  • 현창구
  • 고민녕

Assignees

  • 제주대학교 산학협력단

Dates

Publication Date
20260511
Application Date
20230414

Claims (10)

  1. delete
  2. delete
  3. A pharmaceutical composition for the prevention or treatment of hyperpigmentation comprising, as an active ingredient, one or more selected from the group consisting of the strain of the genus Viridibacillus JNUCC6 ( Viridibacillus sp. JNUCC6) of accession number KCTC15046BP, a lysate thereof, a culture medium, an extract of the culture medium, and a fermented liquid.
  4. A cosmetic composition for skin whitening comprising, as an active ingredient, one or more selected from the group consisting of the strain of the genus Viridibacillus JNUCC6 ( Viridibacillus sp. JNUCC6) of accession number KCTC15046BP, a lysate thereof, a culture medium, an extract of the culture medium, and a fermented liquid.
  5. A topical skin preparation for preventing or improving hyperpigmentation comprising, as an active ingredient, one or more selected from the group consisting of the strain of the genus Viridibacillus JNUCC6 ( Viridibacillus sp. JNUCC6) of accession number KCTC15046BP, a lysate thereof, a culture medium, an extract of the culture medium, and a fermented liquid.
  6. delete
  7. A pharmaceutical composition for the prevention or treatment of inflammatory diseases comprising, as an active ingredient, one or more selected from the group consisting of the strain of the genus Viridibacillus JNUCC6 ( Viridibacillus sp. JNUCC6) of accession number KCTC15046BP, a lysate thereof, a culture medium, an extract of the culture medium, and a fermented liquid.
  8. A topical skin preparation for the prevention or treatment of inflammatory diseases comprising, as an active ingredient, one or more selected from the group consisting of the strain of the genus Viridibacillus JNUCC6 ( Viridibacillus sp. JNUCC6) of accession number KCTC15046BP, a lysate thereof, a culture medium, an extract of the culture medium, and a fermented liquid.
  9. An anti-inflammatory cosmetic composition comprising one or more active ingredients selected from the group consisting of the strain of Viridibacillus sp. JNUCC6 ( Viridibacillus sp. JNUCC6) of the genus Viridibacillus with accession number KCTC15046BP, a lysate thereof, a culture medium, an extract of the culture medium, and a fermented liquid.
  10. delete

Description

Viridibacillus sp. JNUCC6 strain having skin whitening and anti-inflammatory activity and uses thereof The present invention relates to a strain of the genus Viridibacillus JNUCC6 having skin whitening and anti-inflammatory activity, and other uses. Natural ingredients are increasingly being used in existing cosmetics and personal care products. Companies are replacing synthetic chemicals with natural ingredients to meet consumer demands and utilize sustainable materials. Many biological components from plants and various other organisms are major sources of natural ingredients. Natural cosmetics have long used plant and animal extracts as primary ingredients. However, microorganisms, such as bacterial cells, are now considered a potential sustainable source of functional ingredients or additives to improve the quality of these products. Furthermore, these products can be easily produced commercially. While diverse microorganisms exist in nature, very few are commercially utilized in the cosmetics industry. Therefore, the abundant yet underutilized biodiversity of microorganisms represents potential opportunities for future biotechnology and cosmetic applications. Chemical research on biologically active compounds isolated from microorganisms has accelerated tremendously in recent years. The increasing demand for biomolecules is primarily due to their potential efficacy in cosmetics, pharmaceuticals, fine chemicals, and functional personal care products. Microorganisms are a valuable resource because they produce metabolites that can be mass-produced at a reasonable cost. Furthermore, a major advantage of microbial ingredients is their biocompatibility. Other benefits include simplified processes, improved and consistent product quality, and a small environmental footprint. Bacteria secrete many biologically active compounds with significant commercial value. These compounds are applied in various cosmetics used for beautification or to improve the health of subjects, replacing chemical ingredients. The global beauty industry features a diverse range of whitening functional cosmetics, and their market share is expected to expand further. The cosmetics industry has introduced various chemical additives, including hydroquinone and arbutin, to meet the increasing demand for skin whitening agents. These compounds exhibit excellent whitening effects. However, some of these drugs are associated with toxic side effects. Through the efforts of researchers worldwide to address this problem, whitening active substances derived from natural products that are less toxic and have fewer side effects have been discovered [Non-patent Literature 1]. In addition, as the number of consumers who consider the safety of cosmetics important increases, green and eco-friendly organic products are gaining popularity. Ultimately, research and discovery regarding safe and healthy skin whitening ingredients have become a trend of modern development [Non-patent Literature 2]. Hyperpigmentation is one of the most common skin conditions affecting both men and women of all races due to various factors such as ultraviolet (UV) exposure and skin inflammation. Topical whitening agents have been reported to be the most superior and less aggressive treatment for hyperpigmentation compared to instrumental approaches. Hyperpigmentation is associated with a decrease in the production of melanin, the primary pigment that regulates skin and hair color. Excessive melanin production leads to its accumulation in the skin, forming freckles that can cause serious skin cancer. Therefore, it is necessary to inhibit melanin production to prevent excessive skin pigmentation. Additionally, good anti-inflammatory effects can reduce skin damage caused by oxidation and inflammation, thereby exhibiting a synergistic whitening effect [Non-patent Literature 3]. The genus Viridibacillus belongs to the phylum Bacillus, and the family Caryophanaceae was first proposed to reclassify three species of the genus Bacillus. To date, there are three strains in this genus: Viridibacillus arenosi (DSM 16319), Viridibacillus arbi (DSM 16317), and Viridibacillus naidey (DSM 15031). According to recent research, the genus Viridibacillus is used not only for the production of green silver nanoparticles (G-AgNPs), which are receiving much attention in the medical community for their excellent efficacy against multidrug-resistant microorganisms, but also for the production of environmentally friendly, economical, reliable, and controlled cadmium sulfide particles [Non-patent Literature 4]. Therefore, genetic, physiological, and ecological information on Viridibacillus is still lacking, and its application to human health is still in the early stages. Figure 1 shows the inhibition of melanin production and tyrosinase activity by JNUCC6 extract in B16F10 melanoma cells [(a) Cell viability was evaluated using the MTT assay and expressed as a percentage value compared to the untreated g