KR-102962312-B1 - Schizophyllum commune GFC-SC_240704 Strain and Cosmetic Composition Comprising Schizopyrin Isolated Therefrom

Abstract

The present invention relates to a cosmetic composition comprising *Cyzophyllum commune* and *Cyzophyllan* derived therefrom, and more specifically, to a cosmetic composition comprising a novel strain of *Cyzophyllum commune* developed from *Cyzophyllum citrus* isolated from nature and *Cyzophyllan* derived therefrom. The cosmetic composition provided by the present invention has excellent moisturizing, antibacterial, skin barrier improvement, skin regeneration, and wound healing effects.

Inventors

• 김수연
• 한우리자랑
• 정연선
• 민진우
• 강희철

Assignees

• (주)지에프씨생명과학

Dates

Publication Date

20260511

Application Date

20250729

Claims (5)

1. (a) antimicrobial activity against Escherichia coli ( E. coli ), Pseudomonas aeruginosa ( P. aeruginosa ), or Candida albicans ( C. albicans ); (b) moisturizing; (c) skin barrier improvement; (d) skin regeneration; and (e) wound healing activity. A hybrid variety, *Sizophyllum commune* GFC-SC_240704 (Accession No.: KACC 93434P).
2. delete
3. A cosmetic composition comprising, as an active ingredient, schizophyllan isolated from the hybrid variety of claim 1, *Sizophyllum commune* GFC-SC_240704 (accession number: KACC 93434P) or a culture thereof, and having (a) antibacterial activity against * Escherichia coli *, *Pseudomonas aeruginosa *, or *Candida albicans *; (b) moisturizing activity; (c) skin barrier improvement activity; (d) skin regeneration activity; and (e) wound healing activity.
4. A cosmetic composition according to claim 3, characterized in that the culture is a culture of *Cyzophyllum commune* GFC-SC_240704 (accession number: KACC 93434P) cultured in a medium containing soy peptone and dextrose.
5. In paragraph 3, the cosmetic composition is a formulation selected from the group consisting of liquids, gels, solids, patches, and sprays.

Description

Schizophyllum commune GFC-SC_240704 strain and cosmetic composition comprising schizopyrin isolated therefrom The present invention relates to a cosmetic composition comprising *Cyzophyllum commune* and *Cyzophyllan* derived therefrom, and more specifically, to a cosmetic composition comprising a novel strain of *Cyzophyllum commune* developed from *Cyzophyllum citrus* isolated from nature and *Cyzophyllan* derived therefrom. Skin moisturization and antioxidant properties have been subjects of consistent interest in the cosmetics industry. Driven by this interest, the demand for products offering moisturization and antioxidant capabilities is steadily increasing, and the related cosmetics industry is growing into a high-value-added sector. Glucan constitutes the cell walls of fungi and yeast and is the major polysaccharide found in mushrooms; it is classified into alpha-glucan and beta-glucan based on the bonding method and structure of its monomers. Alpha-glucans consist of monomer molecules linked via α-1,4 or α-1,6 bonds, with well-known examples including starch and glycogen. Beta-glucans consist of monomer molecules linked via β-1,3, β-1,4, or β-1,6 bonds, and are connected by β-glycosidic bonds. Various types of beta-glucans exist in grains, yeast, mushrooms, some bacteria, and seaweed; each origin exhibits different forms, such as primary structure, branching pattern, molecular weight, and solubility, and their biological characteristics also differ. Among them, mushroom β-glucans form a stabilized, complex tertiary structure in which the backbone of glucose residues is connected by β-(1→3)-glycosidic bonds and β-(1→6) residues are attached (Zhu, F., Du, B., Bian, Z., & Xu, B. (2015). Beta-glucans from edible and medicinal mushrooms: Characteristics, physicochemical and biological activities. Journal of Food Composition and Analysis, 41, 165-173.) The efficacy and activity of beta-glucans derived from various mushrooms, such as lentinan from shiitake mushrooms ( Lentinula edodes ), ganoderan from reishi mushrooms ( Ganoderma lucidum ), and grifolan from cloud mushrooms ( Coriolus versicolor ), are being studied. Among them, schizophyllan refers to the beta-glucan produced through the shiitake mushroom. Schizophyllum commune is a mushroom of the family Schizophyllaceae, order Agaricomycetes, class Basidiomycota. It is a white-rot fungus that attaches to dead and living trees and extends white mycelia, using the lignin and cellulose of wood for nutrients. The cap is an irregular fan shape, approximately 1 to 5 cm in diameter. It grows long with the cap splitting into two halves. This characteristic is reflected in the name *Schizophyllum*; "Schizo" means "split" or "divided," and "phyllum" is the Greek word for "leaf." The fruiting body is white, gray, or grayish-brown, and its tissue is rather tough and leathery. It also possesses excellent resilience, capable of returning to its original state after drying out if moisture is replenished over time. The spores are generally irregular in shape, primarily oval or circular. They measure 3–4 × 1–1.5 µm and are also generally capable of surviving in dry environments. The shiitake mushroom follows the tetrapolar mating type typical of basidiomycetes. Consequently, among the basidiospores formed in the fruiting body, haploid cells (homokaryons) with different karyotypes grow in an n + n pattern, developing into new polyploid cells (heteroaryons). Subsequently, under appropriate temperature, humidity, and culture conditions, it undergoes a life cycle in which it forms fruiting bodies and produces new basidiospores as basidia develop. Schizophyllan, a beta-glucan produced from the shiitake mushroom, is a nonionic, water-soluble beta-glucan that exhibits characteristic viscosity and is used as a thickening agent and moisturizer in the cosmetics industry. In the pharmaceutical field, it is used as an immune response modulator and immune enhancer, and much research is being conducted on vaccines and anticancer drugs. Korean Registered Patent No. 1787393 disclosed a cosmetic composition containing a culture solution obtained by culturing shiitake mushroom mycelium in a fermented red ginseng product, and Korean Registered Patent No. 2057829 disclosed a cosmetic composition containing beta-glucan isolated from shiitake mushroom mycelium or a culture thereof as an active ingredient, and a vacuum-filled ampoule containing the same. However, these methods utilized beta-glucan isolated from the mycelium of the common shiitake mushroom, and research is needed to isolate sizophyllan, a beta-1,3 glucan, with a higher yield. Accordingly, the inventors obtained a new strain by crossbreeding naturally isolated shiitake mushrooms, and confirmed that schizophyllan obtained from a culture of this strain has moisturizing, antibacterial, skin barrier improvement, skin regeneration, and wound healing effects, thereby completing the present invention. Figure 1 is a clamp connection m