KR-102962754-B1 - METHOD OF CONTROLLING GENE EXPRESSION OF CANCER CELL DNA USING TERAHERTZ WAVE

Abstract

The present invention relates to a method for controlling gene expression in cancer cells that can control demethylation of cancer cells without damaging DNA. The method for controlling gene expression in cancer cells according to the present invention comprises: (a) preparing a control sample cancer cell and a first sample cancer cell to a Nth sample cancer cell; (b) irradiating each of the first sample cancer cell to the Nth sample cancer cell with terahertz waves at different powers; (c) detecting the expression level of a predetermined gene in each of the first sample cancer cell to the Nth sample cancer cell by comparing the first sample cancer cell to the Nth sample cancer cell, to which terahertz waves at different powers were irradiated in step (b), with the control sample cancer cell; and (d) irradiating a target cancer cell with terahertz waves at the power of the terahertz waves irradiated to the sample cancer cell having a predetermined expression level relative to the control sample cancer cell among the first sample cancer cell to the Nth sample cancer cell, thereby expressing a predetermined gene in the target cancer cell to the predetermined expression level (N is a natural number).

Inventors

• 손주혁

Assignees

• 서울시립대학교 산학협력단

Dates

Publication Date

20260507

Application Date

20221209

Claims (6)

1. (a) A step of preparing control sample cancer cells and first sample cancer cells to Nth sample cancer cells; (b) a step of irradiating each of the first sample cancer cells to the Nth sample cancer cells with terahertz waves at different powers; (c) a step of detecting the expression level of a predetermined gene in each of the first sample cancer cells to the Nth sample cancer cells, which have been irradiated with terahertz waves of different power in step (b) above, by comparing them with the control sample cancer cells; and (d) a step of irradiating a target cancer cell with terahertz waves using the power of terahertz waves irradiated to a sample cancer cell having a predetermined expression level relative to the control sample cancer cell among the first sample cancer cell to the Nth sample cancer cell, thereby expressing a predetermined gene of the target cancer cell to the predetermined expression level. A method for controlling gene expression in cancer cells characterized by including (N is a natural number).
2. In paragraph 1, After performing step (b) above, the step of storing the first sample cancer cells to the Nth sample cancer cells for a predetermined period of time A method for controlling gene expression in cancer cells characterized by further including
3. In paragraph 2, A method for controlling gene expression in cancer cells, characterized in that the above-mentioned predetermined time is within 48 hours.
4. In paragraph 1, A method for controlling gene expression in cancer cells, characterized in that the first to Nth sample cancer cells each comprise melanoma cells.
5. In paragraph 2, A method for controlling gene expression in cancer cells, characterized in that the first to Nth sample cancer cells each comprise melanoma cells.
6. In paragraph 1, A method for controlling gene expression in cancer cells, characterized in that the target cancer cells include melanoma cells.

Description

Method of Controlling Gene Expression of Cancer Cells Using Terahertz Waves The present invention relates to a method for controlling gene expression in cancer cells. Cancer cells possess the ability to self-renewal and differentiate into various cell types, leading to active research on their utilization in the treatment of degenerative diseases. While cancer cell-based therapies initially garnered high expectations, reports indicate that the regeneration of damaged tissue falls short of expectations or that the effects are not sustained. This is due to the low survival rate and the low ability of transplanted adult cancer cells to differentiate into desired cell types. Epigenetic modification is recognized as a key factor in the differentiation of cancer cells. Regulating the gene activity of cancer cells allows for the control of embryonic development and the specific cell type and differentiation stage. In other words, if the gene activity of cancer cells is appropriately controlled, they can be regulated to differentiate into a desired cell lineage. Although numerous studies are being conducted to control cancer cell expression using enzymatic biochemical reactions, there are problems such as low reproducibility and the occurrence of DNA damage. Therefore, it is necessary to develop cell therapies that maximize the efficiency of cancer cell treatment through the development of technology that more precisely controls the differentiation of cancer cells. FIG. 1 is a flowchart illustrating a method for controlling gene expression in cancer cells according to the present invention. FIG. 2 is a schematic diagram illustrating step S200 of a method for controlling gene expression in cancer cells according to the present invention. FIG. 3 is a graph showing the number of FC>2 genes over time according to the present invention. FIGS. 4a to 4c are graphs illustrating the gene expression levels of JUN, FOS, and CXCL8 according to the gene expression control method of cancer cells of the present invention. Hereinafter, a method for controlling gene expression in cancer cells according to the present invention will be described in detail with reference to the attached drawings. First, the method for controlling gene expression in cancer cells according to the present invention is performed in a terahertz wave irradiation device, a methylation level detection device, etc. FIG. 1 is a flowchart illustrating a method for controlling gene expression in cancer cells according to the present invention. Referring to FIG. 1, a control sample cancer cell and a first sample cancer cell to the Nth sample cancer cell are prepared (S100) (N is a natural number greater than or equal to 1). The control sample cancer cell and the first sample cancer cell to the Nth sample cancer cell are substantially the same cancer cell. According to the present invention, the control sample cancer cell is not exposed to terahertz waves, whereas the first sample cancer cell to the Nth sample cancer cell are cancer cells to which terahertz waves are irradiated. Here, it is preferable that the control sample cancer cell, the first sample cancer cell to the Nth sample cancer cell are each melanoma cells. Next, terahertz waves are irradiated to each of the first sample cancer cells to the Nth sample cancer cells (S200). Step S200 is explained in detail with reference to Fig. 2. FIG. 2 is a schematic diagram illustrating step S200 of a method for controlling gene expression in cancer cells according to the present invention. Referring to FIG. 2, terahertz waves are irradiated to the first sample cancer cell to the Nth sample cancer cell with the first power to the Nth power, respectively. Specifically, terahertz waves of the first power are irradiated to the first sample cancer cells, and terahertz waves of the second power are irradiated to the second sample cancer cells. Likewise, terahertz waves of the (N-1) power are irradiated to the (N-1) sample cancer cells, and terahertz waves of the N power are irradiated to the N sample cancer cells. Here, the power of the terahertz waves can be appropriately selected as needed and is not limited to a specific value. Next, the first sample cancer cells to the Nth sample cancer cells, which were irradiated with terahertz waves in step S200, are stored until a predetermined time has elapsed (S300). Here, it is preferable that the predetermined time be within 48 hours. The reason for storing the first sample cancer cells to the Nth sample cancer cells irradiated with terahertz waves until a predetermined time has elapsed is that, as illustrated in FIG. 3, gene expression changes over time. This will be described later. Next, in step S200, terahertz waves of the first to Nth power are irradiated, and in step S300, the first to Nth sample cancer cells after a predetermined time has elapsed are compared with the control sample cancer cells (S400). When terahertz waves are irradiated to the sample cancer cells, a