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KR-102962813-B1 - Cosmetic composition for improving skin Moisturizing and wound-healing comprising Hydrangea serrata extract as active ingredients

KR102962813B1KR 102962813 B1KR102962813 B1KR 102962813B1KR-102962813-B1

Abstract

The present invention relates to a cosmetic composition for promoting skin moisturization or improving skin wounds and a topical skin composition containing Hydrangea serrata extract as an active ingredient. A cosmetic composition containing Hydrangea serrata extract of one aspect was found to effectively increase the expression of skin moisturization-related factors, activate the expression of signaling proteins involved in moisturization, and increase skin wound healing without cytotoxicity; thus, it can be effectively utilized as a cosmetic composition for wound healing or moisturization.

Inventors

  • 홍성윤
  • 김수지
  • 이준배
  • 박명삼

Assignees

  • 코스맥스 주식회사

Dates

Publication Date
20260512
Application Date
20221222

Claims (8)

  1. A cosmetic composition for improving skin wounds caused by physical damage, comprising Hydrangea serrata extract as an active ingredient.
  2. A cosmetic composition according to claim 1, wherein the hydrangea extract is extracted with hot water.
  3. A cosmetic composition according to claim 1, wherein the hydrangea extract is included at a concentration of 0.1 ppm to 25 ppm with respect to the total weight of the cosmetic composition.
  4. A cosmetic composition according to claim 1, wherein the hydrangea extract is included in an amount of 0.1 to 10 weight% based on the total weight of the cosmetic composition.
  5. A cosmetic composition according to claim 1, wherein the hydrangea extract promotes the expression of one or more selected from the group consisting of keratin 5, keratin 6, and keratin 16.
  6. delete
  7. A cosmetic composition according to claim 1, wherein the wound is one type selected from the group consisting of a cut, incision, stab wound, split wound, squash, laceration, puncture wound, and puncture wound.
  8. A pharmaceutical composition for treating skin wounds caused by physical damage, comprising Hydrangea serrata extract as an active ingredient.

Description

Cosmetic composition for improving skin moisturizing and wound-healing comprising Hydrangea serrata extract as active ingredients The present invention relates to a cosmetic composition for promoting skin moisturization or improving skin wounds and a skin external application composition containing Hydrangea serrata extract as an active ingredient. The skin is largely composed of three layers: the epidermis, dermis, and subcutaneous fat, and plays an important role in preventing the loss of water and electrolytes and protecting the human body from external stimuli. Damage to such skin tissue constitutes a wound, and wound healing is an essential response to restore the function and morphological characteristics of the damaged tissue to normal. Since failure to treat a wound properly can lead to further damage, such as bacterial infection, it is important to treat it promptly. When a wound occurs, an inflammatory response takes place, during which bacteria or tissue debris are eliminated by phagocytosis, and various cytokines are released from macrophages, triggering the migration and division of cells differentiating during the proliferation phase. Epidermal Growth Factor (EGF) is one of the growth factors that regulate cell proliferation, differentiation, and survival. It performs an excellent function in wound healing by promoting the proliferation and migration of keratinocytes and inducing re-epithelialization. EGF increases the expression of keratin 6 and keratin 16 and activates cell proliferation signaling pathways. Clinical studies have shown that applying EGF to chronic wounds promotes epithelialization, thereby accelerating the speed of wound healing. Hyaluronic acid (HA) is a component of the extracellular matrix and has been reported to be involved not only in water retention, maintaining intercellular spacing, and the storage and diffusion of cell growth factors and nutrients, but also in cell division, differentiation, and migration. A decrease in hyaluronic acid in the skin is a direct cause of the decline in skin elasticity and reduced moisture content associated with aging. Hyaluronic acid synthesis proceeds on the inner surface of the cell membrane by hyaluronic acid synthase (HAS), and it is known that during synthesis, it penetrates the cell membrane and accumulates in the extracellular matrix. Meanwhile, * Hydrangea serrata * belongs to the Saxifragaceae family and is distributed in Korea and Japan. Its flowers are hermaphrodite, appearing in large, flat inflorescences at the tips of branches grown in July and August. The flowers are hairy, and the surrounding sterile flowers have 3 to 5 petals and are bluish-green. The leaves contain the sweetening components phyllodulcin and isophilodulcin, as well as hydrangenol, rutin, kaempferol, and caffeic acid. They are known to be effective against inflammation, antioxidant activity, and photoaging, but their effects on wound healing or skin moisturization are not known. Figure 1 is a figure showing the formulation stability of a cream-type cosmetic composition prepared using a manufactured hydrangea ethanol extract and a hydrangea hot water extract. Figure 2 shows the results of confirming cytotoxicity according to treatment with different concentrations of hydrangea extract in HaCaT cells. Figure 3 is a figure showing the results of confirming the mRNA expression levels of HAS-1 (Figure 3A), HAS-2 (Figure 3B), and HAS-3 (Figure 3C) according to treatment with different concentrations of hydrangea extract in HaCaT cells. Figure 4 shows the results of confirming the mRNA expression levels of HYAL1 (Figure 4A), HYAL 2 (Figure 4B), and HYAL 4 (Figure 4C) according to treatment with different concentrations of hydrangea extract in HaCaT cells. Figure 5 shows the results of the confirmation of the wound healing migration assay following treatment with hydrangea extract in wound-induced HaCaT cells. Figure 6 shows the results of confirming the mRNA expression levels of KRT5 (Figure 6A), KRT6 (Figure 6B), and KRT16 (Figure 6C) following treatment with hydrangea extract in HaCaT cells. Figure 7 shows the results of treating HaCaT cells with hydrangea extract, confirming the activation of the Activator protein (AP)-1 promoter through a Luciferase reporter assay. Figure 8 shows the effect of increasing the phosphorylation of intracellular AKT/PI3K signaling proteins AKT, PI3K, and PDK by hydrangea extract. Figure 9 shows the effect of increasing the phosphorylation of intracellular AP-1 signaling proteins ERK, JNK, p38, and c-Fos by hydrangea extract. Figure 10 shows the effect of increasing HAS-1, 2, and 3, which are skin moisturizing proteins, by hydrangea extract. Hereinafter, the present invention will be described in detail with reference to examples in order to specifically explain the invention. However, the embodiments according to the present invention may be modified in various different forms, and the scope of the present invention should not