KR-102962881-B1 - Corynebacterium matruchotii strain and its use for improving skin conditions

Abstract

The present invention relates to a novel strain of Corynebacterium matruchotiii, its lysate, culture medium, extract of the culture medium, and the use thereof for improving skin condition. Since the strain has an effect of improving skin condition, it can be usefully used in cosmetics, pharmaceuticals, food, etc.

Inventors

• 김혜빈
• 이동걸
• 강승현

Assignees

• 코스맥스 주식회사

Dates

Publication Date

20260512

Application Date

20230703

Claims (10)

1. Corynebacterium matruchotii CM38 strain deposited under accession number KCCM13240P.
2. delete
3. Claim 1, wherein the strain is a strain that reduces or inhibits melanin production.
4. Culture solution of the strain of claim 1.
5. A cosmetic composition for improving skin condition comprising the Corynebacterium matruchotii CM38 strain deposited under accession number KCCM13240P, its lysate, culture medium, extract of the culture medium, or a mixture thereof, A cosmetic composition in which the improvement of the above skin condition is skin whitening.
6. delete
7. A cosmetic composition according to claim 5 that reduces or inhibits melanin production.
8. A topical skin composition for the prevention or treatment of hypermelanosis, comprising the Corynebacterium matruchotii CM38 strain deposited under accession number KCCM13240P, its lysate, culture medium, extract of the culture medium, or a mixture thereof, A topical skin composition wherein the above-mentioned melanin hyperpigmentation disorder is any one selected from the group consisting of freckle; melasma; chloasma; liver spot; nevus; solar lentigo; melanosis; Peutz-Jeghers syndrome; chloasma gravidarum; hyperpigmentation after drug use; and postinflammatory hyperpigmentation.
9. A pharmaceutical composition for the prevention or treatment of hypermelanosis, comprising the Corynebacterium matruchotii CM38 strain deposited under accession number KCCM13240P, its lysate, culture medium, extract of the culture medium, or a mixture thereof, A pharmaceutical composition wherein the above-mentioned melanin hyperpigmentation disorder is any one selected from the group consisting of freckle; melasma; chloasma; liver spot; nevus; solar lentigo; melanosis; Peutz-Jeghers syndrome; chloasma gravidarum; hyperpigmentation after drug use; and postinflammatory hyperpigmentation.
10. A food composition for improving skin condition comprising the Corynebacterium matruchotii CM38 strain deposited under accession number KCCM13240P, its lysate, culture medium, extract of the culture medium, or a mixture thereof, A food composition in which the improvement of the above skin condition is skin whitening.

Description

Corynebacterium matruchotii strain and its use for improving skin conditions The invention relates to a novel microorganism, its lysate, culture medium, extract of the culture medium, and the use thereof for improving skin conditions. The skin's ecosystem provides diverse forms of habitats for microorganisms, supporting a wide range of species. These microorganisms form a symbiotic relationship with their human hosts and are known to exert many positive effects on the host. The skin comprises various habitats, such as invaginations and specialized niches, facilitating the growth of a wide distribution of microorganisms. Fundamentally, the skin forms a physical barrier that helps defend against potential external hazards and toxins. It serves as a point of contact with the external environment and is also a habitat for various microorganisms (e.g., fungi, bacteria, viruses, and small larvae). Microorganisms adapt to specialized niches to establish habitats based on their physical and chemical needs. Generally, the skin is cool, acidic, and remains dry. Structurally, the epidermis forms the skin barrier, playing a crucial role in blocking the penetration of microorganisms and toxins while retaining moisture. The uppermost layer of the epidermis is composed of the stratum corneum. The epidermis has a structure known as a 'brick and mortar structure,' and the skin tissue undergoes a continuous self-repair process, while the scales (squames) that have completed the differentiation process are constantly shed from the skin tissue. Probiotics is a collective term for microorganisms that exert beneficial effects on the human body. Most probiotics known to date are lactic acid bacteria. While it has been reported that probiotics produce effective efficacy through various beneficial actions, research on the interaction between skin commensal bacteria and the skin remains insufficient. Therefore, there is a need to develop skin commensal bacteria that can be usefully applied to skin-related conditions. Figure 1 is a graph showing the amount of melanin produced (%). None: α-MSH untreated + sample untreated group, Control: α-MSH treated + medium treated group (negative control), Arbutin (100 ppm): α-MSH treated + arbutin treated group (positive control), C. matruchotii (1%): α-MSH treated + strain culture solution (Example 2) treated group. The following examples will be explained in more detail. However, these examples are intended to illustrate one or more specific examples, and the scope of the present invention is not limited to these examples. [Example] Example 1. Isolation and Identification of Strains A strain of Corynebacterium matruchotii was isolated and identified from the skin of a healthy woman. Specifically, the cheeks, tip of the nose, and nostril borders of healthy women were swabed 2 to 3 times each using a sterile cotton swab soaked in PBS (phosphate buffer saline). The swab was placed in a tube containing PBS and vortexed for 10 seconds. 10-, 100-, and 1,000-fold dilutions of the samples were prepared, and 100 μL of each was added to 0.1X BHI (Brain Heart Infusion) Broth and 0.5X R2A (Reasoner's 2A) agar medium, respectively, and spread using a spreader. After incubating the plates at 30°C or 37°C, colonies were isolated. 16S rRNA gene sequence identification was performed on the colonies after incubation. The primers used were designed to react and amplify only bacteria, and the primer sequences are as follows: 27F, Forward primer: 5'-AGAGTTTGATCCTGGCTCAG-3' (Sequence No. 2); 1492R, reverse primer: 5'-GGTTACCTTGTTACGACTT-3' (Sequence No. 3). PCR amplification was performed for 30 cycles of 95°C for 1 minute, 55°C for 1 minute, and 75°C for 1 minute 30 seconds, followed by treatment at 72°C for 8 minutes and storage at 4°C. After the PCR reaction was completed, the DNA sequences of the isolated cultured microorganisms were determined using ABI-3730XL (ABI, USA). Using the 16S-based ID program provided on the Korean EZbiocloud website, the nucleotide sequences of the 16S rRNA region were compared and analyzed with other strains. Among them, a novel microorganism , Corynebacterium matruchotii CM38 strain, was selected, which has 99.58% homology with the Corynebacterium matruchotii type strain. A selected strain of Corynebacterium matruchotiii was deposited with the Korean Culture Center of Microorganisms (KCCM) on September 15, 2022, and was assigned accession number KCCM13240P. The above-mentioned strain of Corynebacterium matruchotiii has the 16S rRNA sequence of SEQ ID NO. 1 (complementary DNA). Example 2. Preparation of strain culture solution A culture solution of the Corynebacterium matruchotii strain was prepared. Specifically, the Corynebacterium matruchotiii CM38 strain isolated in Example 1 above was cultured in Brain Heart Infusion (BHI) medium. Cells were removed by centrifugation, and the supernatant was recovered and filtered to prepare a culture solution of the Corynebacterium matruchotii