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KR-102962916-B1 - Protection of bioactive substances and/or their precursors

KR102962916B1KR 102962916 B1KR102962916 B1KR 102962916B1KR-102962916-B1

Abstract

The present invention relates to a method for producing an emulsion or suspension from biomass. The present invention also relates to the production of a powder or a product produced therefrom. The present invention also relates to an emulsion or suspension produced by a method as described herein. The present invention also relates to a powder or a product produced therefrom produced by a method as described herein.

Inventors

  • 어거스틴 마리 앤
  • 상구안스리 루즈

Assignees

  • 커먼웰쓰 사이언티픽 앤드 인더스트리얼 리서치 오가니제이션

Dates

Publication Date
20260511
Application Date
20190201
Priority Date
20180202

Claims (20)

  1. A method for producing a powder containing an entrapped or encapsulated bioactive, wherein the method i) a step of obtaining an aqueous mixture containing proteins and carbohydrates from the biomass of a single-species organism; ii) A step of adding exogenous oil to an aqueous mixture; iii) a step of forming an emulsion or suspension containing a bioactive substance; and iv) a step of forming a powder comprising a bioactive substance that is entrapped or encapsulated from an emulsion or suspension. Includes, A method in which the bioactive substance is an exogenous oil and the biomass is Brassic a.
  2. In paragraph 1, the powder has the following characteristics: a) an induction period of 10 to 300 hours when measured at an initial oxygen pressure of 80°C and 5 bar; b) 5% w/w to 50% w/w oil; c) 10% w/w to 40% w/w of oil; and d) The oil content of the emulsion or suspension before forming the powder is 1% w/w to 10% w/w. A method comprising one or more of the following.
  3. In paragraph 1, The aqueous mixture additionally contains proteins and carbohydrates from at least one additional biomass from a single species of organism, or a) Biomass or additional biomass comprises a protein-to-carbohydrate ratio of 1:1 to 1:10.5; or b) Additional biomass is Brassicaceae , method.
  4. The method according to claim 1, wherein the exogenous oil comprises a fatty acid, the fatty acid is an omega-3, omega-6, or omega-9 fatty acid, and the omega-3 fatty acid is one or more of α-linolenic acid (ALA), eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA).
  5. In paragraph 1, a) A step of pre-treating biomass, wherein the step of pre-treating i) Heating; ii) Macerating; iii) Microwaving; iv) Exposure to low-frequency sound waves (ultrasound); v) Pulsed electric field processing; vi) Static high pressure; vii) Extrusion; viii) Enzyme treatment; ix) Fermentation; x) extraction or separation process; and xi) Drying A step comprising one or more of the following; and/or b) a step of post-treating the emulsion or suspension to reduce microbial activity, wherein the step of post-treating i) Heating; ii) Microwaving; iii) UV treatment; and iv) High-pressure processing Steps that include one or more of A method that additionally includes
  6. In paragraph 1, the exogenous oil has the following characteristics: a) containing one or more fatty acids; b) selected from one or more of fish oil, krill oil, oxalic acid oil, canola oil, sunflower oil, avocado oil, soybean oil, borage oil, evening primrose oil, safflower oil, flaxseed oil, olive oil, pumpkin seed oil, hemp seed oil, wheat germ oil, palm oil, palm olein, palm kernel oil, coconut oil, medium-chain triglycerides, and grapeseed oil; and c) The exogenous oil is a fish oil or aquatic oil, and the fish oil or aquatic oil is selected from one or more of tuna oil, herring oil, mackerel oil, sardine oil, cod liver oil, menhayden oil, shark oil, algae oil, squid oil, and squid liver oil. A method having one or more of the following.
  7. In paragraph 1, The step of forming the powder includes spray drying, freeze drying, refractance window drying, or drum drying, and/or, A method in which the intrapped or encapsulated bioactive material is resistant to degradation for 3 to 24 months.
  8. In paragraph 1, Brassica is the Brassica oleracea variety italica , method.
  9. In paragraph 1, Brassica is a method of the Brassica oleracea variety botrytis .
  10. A powder comprising a bioactive substance that is entrapped or encapsulated, and a protein and a carbohydrate derived from the biomass of a single organism, wherein the bioactive substance is an exogenous oil and the biomass is a brasicacin powder.
  11. In Paragraph 10, The powder has the following characteristics: a) The fact that intrapped or encapsulated bioactive materials are resistant to oxygen degradation compared to non-intrapped or unencapsulated bioactive materials; b) the powder has an induction period of 10 to 300 hours when measured at an initial oxygen pressure of 80°C and 5 bar; c) the powder contains 5% w/w to 50% w/w of oil; d) The powder contains 10% w/w to 40% w/w of oil. Powder having one or more of the following.
  12. A product containing the powder of Clause 10.
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Description

Protection of bioactive substances and/or their precursors The present invention relates to a method for producing an emulsion or suspension from biomass. The present invention also relates to the production of a powder or a product produced therefrom. The present invention also relates to an emulsion or suspension produced by a method as described herein. The present invention also relates to a powder or a product produced therefrom produced by a method as described herein. Bioactives, such as oxygen-sensitive fatty acids and oils, are desirable ingredients for foods, supplements, and/or cosmetics. However, because many oils and bioactive molecules are susceptible to oxidation and degradation reactions when exposed to various environments (e.g., oxygen, heat, pH, or enzymes), these ingredients may degrade before ingestion or use, cannot be stored in a form suitable for ingestion or use, or fail to reach the desired site in the body after ingestion. Oxygen-sensitive oils include those containing polyunsaturated fatty acids, such as omega-3, omega-6, or omega-9 fatty acids. Unstable bioactive ingredients include ingredients that are water-soluble (e.g., polyphenols unstable at high pH), oil-soluble (e.g., oxygen-sensitive carotenes), or sparingly soluble in oil or water (e.g., resveratrol, curcumin). 58. Encapsulation has been used for the protection and delivery of lipophilic and hydrophilic bioactive substances, but there are still difficulties in selecting the most suitable encapsulation system for delivery (References [Augustin and Sanguansri, 2015; McClements, 2015]). Due to the health-promoting properties of omega-3 oils, they are highly susceptible to oxidation, so the stabilization of these oils has received considerable attention (References [Sanguansri and Augustin, 2006; Drusch and Manino, 2007]). Although methods for encapsulating oxygen-sensitive oils and bioactive substances are known, these methods require purified or substantially purified proteins (e.g., isolated whey protein, isolated soy protein, or casein and carbohydrates) which are not economical for many products. Purified proteins and purified carbohydrates can be used alone or in combination as encapsulation matrices for the delivery of bioactive substances (References [Augustin and Hemar, 2009; Aditya et al., 2017]). For example, starch has been commonly used as a wall material for encapsulation (References [Hoyos-Leyva et al., 2018]), and proteins have been found to be useful for delivery due to their own numerous desirable functional properties suitable for encapsulation (References [Subirade and Chen, 2008; Livney, 2010]). In MicroMAX® microencapsulation technology, purified proteins (preferably casein) and purified carbohydrates are used to produce encapsulating agents for oils. Heated protein-carbohydrate formulations ( MicroMAX® microencapsulation technology) have been found to be superior to physical formulations of corresponding proteins and carbohydrates (WO 01/74175; literature [Augustin et al., 2006]). The purified proteins and purified carbohydrates used in this process are selected to be colorless and flavorless, and the purification steps involved in their isolation can be costly. Therefore, there is a need for novel formulations and processes for producing products containing oxygen-sensitive bioactive substances, such as fatty acids and oils, which are easily degraded during storage, processing, and within the gastrointestinal tract. The inventors have developed a method for producing an emulsion, suspension, or powder containing proteins and carbohydrates obtained from a single source. In one embodiment, the present invention provides a method for producing an emulsion or suspension, and the method i) a step of obtaining an aqueous mixture containing proteins and carbohydrates from the biomass of a single-species organism; ii) optionally adding oil to the aqueous mixture; and iii) a step of forming an emulsion or suspension comprising a bioactive substance and/or a bioactive substance precursor. Includes In one embodiment, the aqueous mixture is an aqueous suspension. In one embodiment, the present invention provides a method for producing a powder comprising an entrapped or encapsulated bioactive substance and/or a bioactive substance precursor, and the method i) a step of obtaining an aqueous mixture containing proteins and carbohydrates from the biomass of a single-species organism; ii) A step of adding oil to an aqueous mixture; iii) a step of forming an emulsion or suspension comprising a bioactive substance and/or a bioactive substance precursor; and iv) a step of forming a powder comprising a bioactive substance and/or a bioactive substance precursor that is entrapped or encapsulated from an emulsion or suspension. Includes In one embodiment, the present invention provides a method as described herein, wherein a bioactive substance and/or a bioactive substance precursor is i) Components of biomass; ii) the oi