KR-102963168-B1 - CELL FIXATION DEVICE CAPABLE OF CELL PICKING

KR102963168B1KR 102963168 B1KR102963168 B1KR 102963168B1KR-102963168-B1

Abstract

A cell immobilization device capable of cell picking according to an embodiment of the present invention may include: a gel curing chamber having a hollow portion and a glass for curing a hydrogel disposed on a lower surface open downward; a gel thickness forming part capable of being introduced into the hollow portion of the gel curing chamber and limiting the exposure of the hydrogel, which is placed in a sol state on the glass within the gel curing chamber, to ultraviolet light by a set thickness; and a fixing part for fixing the glass to the gel curing chamber.

Inventors

김승훈
강효정
배가은
노혜란
이정민

Assignees

주식회사 씨티셀즈

Dates

Publication Date: 20260511
Application Date: 20230105
Priority Date: 20220107

Claims (10)

A gel curing chamber having a hollow portion and a glass for curing a hydrogel disposed on a lower surface open downward; A gel thickness forming part capable of being introduced into the hollow portion of the gel curing chamber and limiting the exposure of the hydrogel placed in a sol state on the glass within the gel curing chamber to ultraviolet rays by a set thickness; and A fixing part for fixing the glass to the gel curing chamber; comprising, The above fixing part is characterized by being optionally detachable to separate the gel curing chamber from the glass, thereby facilitating access to the hydrogel cured on the glass. A cell immobilization device capable of cell picking, wherein a cell is immobilized on the upper surface of the glass in contact with the gel curing chamber.
In paragraph 1, The above fixed part is, A base member in which the glass is fixed to an upper surface and the gel curing chamber is disposed on the glass; and An intermediate member having a first through hole formed corresponding to the shape of the gel curing chamber, penetrating the gel curing chamber through the first through hole and positioned on the upper part of the base member; and A cell immobilization device capable of cell picking, characterized by including a fixing member that fixes the position of the glass relative to the gel curing chamber by fixing the intermediate member relative to the base member.
In paragraph 2, The above-mentioned fixing member is, A screw forming portion protruding in the shape of a circular band partially cut from the upper surface of the base member with the glass in between, and having screw threads formed on its outer surface; and A cell fixation device capable of cell picking, characterized by including a clamping portion that fixes the position of the glass relative to the gel curing chamber by fixing the intermediate member to the base member through a second through hole of the intermediate member that is penetrated to correspond to the shape of the screw-forming portion.
In paragraph 3, A cell immobilization device capable of cell picking, characterized in that the upper surface of the base member is provided with an insertion groove into which the glass is inserted.
In paragraph 1, A cell immobilization device capable of cell picking, characterized by further including a sealing portion interposed between the gel curing chamber and the glass to tightly seal the bottom of the gel curing chamber and the glass.
In paragraph 1, A cell immobilization device capable of cell picking, characterized by coating the above glass with an azide-based coating material.
In paragraph 1, A cell immobilization device capable of cell picking, characterized in that the gel thickness forming part is provided with a hydrophobic material for separation from the hydrogel.
In paragraph 1, A cell immobilization device capable of cell picking, characterized in that the gel curing chamber is provided in a black color.
In paragraph 1, A stepped member is provided at the lower part of the inner wall of the gel curing chamber, formed in a stepped manner toward the center of the gel curing chamber. A cell fixation device capable of cell picking, characterized in that a locking member engaging with the step member is provided at the lower end of the outer wall of the gel thickness forming part so that the bottom surface of the gel thickness forming part is spaced apart from the upper surface of the glass.
In Paragraph 9, A cell immobilization device capable of cell picking, characterized in that the forming length of the step member is greater than the forming length of the catch member, so that the lower end of the gel thickness forming part is spaced from the glass in the range of 10 micrometers to 200 micrometers, and a hydrogel is formed in the spaced-out space.

Description

Cell fixation device capable of cell picking The present invention relates to a cell immobilization device capable of cell picking, and more specifically, to a cell immobilization device capable of cell picking that allows for accurate and easy cell separation without cell loss by centrifuging while the glass and gel curing chamber are fixed by a fixing part, and also allows access to the hydrogel while the gel curing chamber is separated from the glass, thereby facilitating the use of a cell picking device for immunostaining or subsequent analysis after immunostaining. Immunocytochemistry (ICC) analysis is an experimental technique that utilizes the property of antibodies to specifically bind to antigens to study and identify the sites of specific substances within tissues or cells. Typically, a method of attaching cells to glass using a cell centrifuge can be used. For example, cells can be attached to glass using cytospin, but in the case of this device, there is a risk that approximately 40% of the total cells may be lost during the centrifugation process. Therefore, when applied to rare cells, accurate analysis may be difficult due to cell loss. Meanwhile, in the case of conventional devices, the chamber is attached to the glass, and while cell picking is possible in this state, there may be limitations in picking cells for subsequent analysis due to the wall structure of the chamber. In other words, the cell picking device has a limitation in that it cannot easily access the cells due to the chamber walls, making proper cell picking difficult and consequently reducing work efficiency. Accordingly, there is a need to develop a cell immobilization device with a novel configuration that minimizes cell loss during centrifugation, enables a uniform and accurate curing process for cell staining, and facilitates smooth cell picking by allowing easy access to the cells. FIG. 1 is an exploded perspective view of a cell immobilization device capable of cell picking according to one embodiment of the present invention. Figure 2 is a cross-sectional view of the combined state of the cell immobilization device of Figure 1. Figure 3 is a schematic diagram illustrating the subsequent process after removing the gel curing chamber from the cell immobilization device of Figure 2. Figure 4 is a schematic diagram illustrating the combined structure of the gel curing chamber and the gel thickness forming part shown in Figure 1. Figure 5 is a perspective view showing the combined structure of Figure 4 in three dimensions. The advantages and/or features of the present invention and the methods for achieving them will become clear by referring to the embodiments described below in detail together with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below but may be implemented in various different forms. These embodiments are provided merely to ensure that the disclosure of the present invention is complete and to fully inform those skilled in the art of the scope of the invention, and the present invention is defined only by the scope of the claims. Throughout the specification, the same reference numerals refer to the same components. Hereinafter, embodiments of the present invention will be described in detail with reference to the attached drawings. FIG. 1 is an exploded perspective view of a cell immobilization device capable of cell picking according to one embodiment of the present invention, FIG. 2 is a cross-sectional view of the assembled state of the cell immobilization device of FIG. 1, and FIG. 3 is a schematic diagram illustrating a subsequent process after removing the gel curing chamber from the cell immobilization device of FIG. 2. Referring to FIGS. 1 and 2, a cell immobilization device (100) capable of cell picking according to one embodiment of the present invention may include a gel curing chamber (110), a gel thickness forming part (120), and a fixing part (150). To explain each configuration, first, the gel curing chamber (110) of the present embodiment may have a column shape with a hollow portion overall. For example, it may have a column shape that is rectangular overall, with the central portion penetrating in the height direction, and may be formed into a rectangular column shape by four chamber walls (111). However, the shape of the gel curing chamber (110) is not limited to the above-mentioned case, and the shape of the chamber (110) can be provided in various ways to suit the imaging range and resolution of the device, such as the hydrogel shape, the concentration of cells (cfu/ml) in the suspension, and the fluorescence microscope for observing stained cells, for example, it may be a cylindrical shape with a hollow part, in which case the glass may be circular. While such a gel curing chamber (110) was conventionally made of, for example, polymethylsiloxane (PDMS), in the present embodiment it may be made of polycarbonate (PC), but any mate