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KR-102963384-B1 - A cosmetic or pharmaceutical composition comprising exosomes derived from Cutibacterium acnes J2K-1573 strain

KR102963384B1KR 102963384 B1KR102963384 B1KR 102963384B1KR-102963384-B1

Abstract

The present invention relates to a cosmetic, pharmaceutical, or quasi-drug composition comprising exosomes derived from the strain Cutibacterium acnes J2K-1573, wherein the exosomes derived from the strain Cutibacterium acnes J2K-1573 of the present invention exhibit excellent efficacy in improving skin wrinkles or skin inflammation.

Inventors

  • 조용한
  • 이혜인
  • 이지연
  • 장준환
  • 배준태

Assignees

  • 주식회사 제이투케이바이오

Dates

Publication Date
20260512
Application Date
20251124

Claims (6)

  1. Step (a) of recovering the culture supernatant by centrifuging the culture of the Cutibacterium acnes J2K-1573 strain of accession number KCTC 16523BP at 9,000–11,000 rpm for 10–20 minutes; Step (b) of obtaining a filtrate by sequentially filtering the culture supernatant recovered in step (a) through 0.65 μm and 0.45 μm; A method for preparing exosomes derived from the strain Cutibacterium acnes J2K-1573 of accession number KCTC 16523BP, characterized by including: step (c) of removing culture medium components and brittle components from the filtrate obtained in step (b) using a molecular weight value 50 kDa filter, and then further filtering with 0.1 μm to obtain a final filtrate.
  2. Step (a) of recovering the culture supernatant by centrifuging the culture of the Cutibacterium acnes J2K-1573 strain of accession number KCTC 16523BP at 9,000–11,000 rpm for 10–20 minutes; Step (b) of obtaining a filtrate by sequentially filtering the culture supernatant recovered in step (a) through 0.65 μm and 0.45 μm; Exosomes derived from the strain Cutibacterium acnes J2K-1573 of accession number KCTC 16523BP obtained from a process comprising: step (c) of removing culture medium components and brittle components from the filtrate obtained in step (b) using a molecular weight value 50 kDa filter, and then further filtering with 0.1 μm to obtain a final filtrate.
  3. A cosmetic composition for improving skin wrinkles comprising exosomes derived from the Cutibacterium acnes J2K-1573 strain of accession number KCTC 16523BP of claim 2.
  4. A cosmetic composition for alleviating inflammation comprising exosomes derived from the Cutibacterium acnes J2K-1573 strain of accession number KCTC 16523BP of claim 2.
  5. delete
  6. An anti-inflammatory composition comprising exosomes derived from the Cutibacterium acnes J2K-1573 strain of accession number KCTC 16523BP of claim 2.

Description

A cosmetic or pharmaceutical composition comprising exosomes derived from Cutibacterium acnes J2K-1573 strain The present invention relates to a cosmetic, pharmaceutical, or quasi-drug composition containing exosomes derived from the Cutibacterium acnes J2K-1573 strain, and more specifically, to a cosmetic, pharmaceutical, or quasi-drug composition containing exosomes derived from the Cutibacterium acnes J2K-1573 strain that has efficacy in improving skin wrinkles or improving skin inflammation. Cutibacterium acnes is an anaerobic Gram-positive bacterium that inhabits the skin and is mainly found deep within hair follicles and pores. C. acnes grows slowly, uses sebum as its primary energy source, produces lipase—a lipid-degrading enzyme—and also has the ability to produce propionic acid. Cutibacterium acnes exists in types A through H. Type A is the most commonly found type and is closely associated with acne and inflammatory lesions, resulting in a high frequency of causing skin diseases. Type H is a strain that helps improve scalp health rather than inducing acne; it is still in the research stage, with extensive studies being conducted on scalp-related aspects such as hair quality, scalp health, and hair growth improvement. Type F is a type that has not been extensively studied at present. According to the results of several studies, the role of Type F may vary depending on skin conditions, such as puberty, aging, and acne. Meanwhile, exosomes are nanovesicles composed of a lipid bilayer structure with a size of approximately 50 to 200 nm, and are substances that play an important role in transmitting signals between cells. Inside exosomes, various nucleic acids such as amino acids, proteins, mRNA, and DNA are contained along with numerous bioactive ingredients beneficial to the skin. Recently, the sources of exosomes have expanded from stem cells to include plants and microorganisms, particularly microbial-derived exosomes isolated during the fermentation process of lactic acid bacteria such as Lactobacillus rhamnosus. These microbial-derived exosomes have been proven to have various skin health improvement effects and beauty synergies, including anti-inflammatory, antioxidant, skin barrier strengthening, skin soothing, cell regeneration, and whitening properties, and are currently gaining attention as new cosmetic materials. In addition, exosomes are highly useful as a target delivery technology (‘delivery system’) for therapeutic ingredients, and their value as biomarkers used as diagnostic indicators for diseases is also receiving significant attention. Therefore, it is expected that the utility of exosome technology will continue to grow across the pharmaceutical and cosmetics sectors in the future. Figure 1 is the identification result of the J2K-1573 strain isolated in the present invention. Figure 2 shows the results of cytotoxicity measurements of exosome samples derived from the J2K-1573 strain before measuring the decrease in MMP-1 mRNA expression. Figure 3 shows the results of measuring the decrease in MMP-1 mRNA expression in exosome samples derived from the J2K-1573 strain. Figure 4 shows the results of measuring the cytotoxicity of exosome samples derived from the J2K-1573 strain before measuring the NO production inhibition effect. Figure 5 shows the results of measuring the NO production inhibitory effect of exosome samples derived from the J2K-1573 strain. In the skin, wrinkles and anti-inflammation play crucial roles in both health and beauty. If wrinkles form and the anti-inflammatory protective function is insufficient, skin aging and various problems occur. Wrinkles form when skin tissue proteins, such as collagen and elastin, are damaged or their quantity decreases, and they also easily develop as the thickness of the skin's elasticity declines during the aging process. Wrinkles are an indicator of skin health, and firm skin makes one look young and healthy. When skin wrinkles are numerous, reduced regenerative capacity, sagging, and loss of elasticity occur, accelerating various forms of skin damage and aging, such as dryness, increased exfoliation, and a weakened skin barrier. Representative cosmetic ingredients known for wrinkle improvement include retinol, peptides, hyaluronic acid, collagen, and vitamins C and E. Anti-inflammation is essential for maintaining normal skin function, protecting the skin barrier, and enhancing regenerative capacity. Chronic inflammation can lead to a decline in skin structure and cellular function, as well as the exacerbation of various skin conditions (such as acne and eczema). Insufficient anti-inflammatory ability can cause skin irritation, worsening of inflammatory lesions, damage to the skin barrier, moisture loss, sensitivity, and reduced skin recovery. Furthermore, persistent chronic inflammation can accelerate aging, result in a darker skin tone, and cause frequent skin breakouts. Known cosmetic ingredients for anti-inflammatory purposes i