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KR-102963411-B1 - PHARMACEUTICAL COMPOSITION COMPRISING THE EXTRACT OF ABIES NEPHROLEPIS AS AN EFFECTIVE COMPONENT FOR PREVENTION OR TREATMENT OF THROMBOSIS AND HEALTH FUNCTIONAL FOOD COMPRISING THE SAME

KR102963411B1KR 102963411 B1KR102963411 B1KR 102963411B1KR-102963411-B1

Abstract

The present invention relates to a pharmaceutical composition and a health functional food for the prevention or treatment of thrombosis containing an extract of Abies nephrolepis as an active ingredient, and more specifically, to a pharmaceutical composition and a health functional food for the prevention or treatment/improvement of thrombosis containing an extract of leaves or branches of Abies nephrolepis as an active ingredient having potent thrombin inhibitory, prothrombin inhibitory, and blood coagulation factor inhibitory activities. The extract of * Abies nephrolepis *, used as an active ingredient in the pharmaceutical composition and health functional food for the prevention or treatment of thrombosis according to the present invention, exhibits strong antithrombotic activity by inhibiting thrombin, an enzyme related to thrombus formation, and blood coagulation factors. At the same time, it has excellent thermal stability and does not lose its inhibitory effects on blood coagulation factors and thrombus-related enzymes even under acidic conditions of pH 2 and in plasma. Therefore, it is expected to be used for the prevention and treatment of thrombosis, such as ischemic stroke and hemorrhagic stroke, through the improvement of blood circulation. Furthermore, since the active ingredient has the excellent effect of being processed into various forms such as extracts, powders, pills, and tablets, allowing for formulation into a form suitable for regular consumption, this invention is highly useful for the pharmaceutical and food industries.

Inventors

  • 손호용
  • 채정우

Assignees

  • 경기도
  • 국립경국대학교 산학협력단

Dates

Publication Date
20260511
Application Date
20230515

Claims (4)

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  3. A blood coagulation inhibitor containing an ethanol extract of fir tree branches as an active ingredient.
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Description

Pharmaceutical composition for the prevention or treatment of thrombosis and health functional food containing Abies nephrolepis extract as an active ingredient The present invention relates to a pharmaceutical composition and a health functional food for the prevention or treatment of thrombosis containing an extract of Abies nephrolepis as an active ingredient, and more specifically, to a pharmaceutical composition and a health functional food for the prevention or treatment/improvement of thrombosis containing an extract of leaves or branches of Abies nephrolepis as an active ingredient having potent thrombin inhibitory, prothrombin inhibitory, and blood coagulation factor inhibitory activities. As a component of the human body, blood possesses various important functions, including the transport of oxygen, nutrients, and waste products, buffering, maintaining body temperature, regulating osmotic pressure and ion balance, maintaining constant fluid levels, regulating humor, maintaining and regulating blood pressure, and providing biological defense. Normal blood circulation is facilitated by the complementary regulation of the blood coagulation and thrombolytic systems within the body. Among these, the mechanism of the blood coagulation system is reported to involve platelets adhering to and aggregating on the blood vessel walls to form a platelet thrombus, after which the blood coagulation system is activated to form a fibrin thrombus centered around the platelet aggregate. The formation of fibrin clots involves a multi-step reaction of numerous blood coagulation factors, through which thrombin, which is involved in fibrin coagulation, is activated. This ultimately leads to the generation of fibrin monomers from fibrinogen. These fibrin monomers are polymerized by calcium and bind to platelets and endothelial cells, forming a permanent blood clot by creating a fibrin polymer cross-linked by Factor XIII. Furthermore, thrombin plays a pivotal role in clot formation by activating platelets, Factor V, and Factor VII to promote blood coagulation reactions. Therefore, thrombin inhibitors can be used as highly useful preventive and therapeutic agents for various thrombotic diseases resulting from excessive blood coagulation abnormalities. Meanwhile, the endogenous thrombosis pathway is known to involve the sequential activation of Factor XII, Factor XI, Factor IX, and Factor X, followed by the activation of prothrombin, which ultimately leads to the activation of thrombin; thus, the specific inhibition of blood coagulation factors is also an important target for the development of treatments for thrombotic diseases. To date, various anticoagulants, antiplatelet agents, and thrombolytics such as heparin, coumarin, aspirin, and urokinase have been used for the prevention and treatment of thrombotic diseases; however, their use is limited due to their very high cost, as well as hemorrhagic side effects, gastrointestinal disorders, and hypersensitivity reactions. Meanwhile, the Korean fir ( Abies nephrolepis ) is an evergreen coniferous tree belonging to the Pinaceae family of the Coniferales order, native to alpine regions in cold areas of Korea, northeastern China, and eastern Siberia. In Korea, it is distributed along the Taebaek Mountains in areas exceeding 1,000 meters above sea level, such as Seoraksan and Taebaeksan, and also grows in the high altitudes of Jirisan Mountain in the southern region. It is a large tree capable of reaching a height of 25 meters, with slender branches and a straight trunk. The flowers are monoecious and bloom in May; the seeds are approximately 6 mm long, the same color as the fruit, triangular, and winged. Typically, Korean fir is used for pulpwood because its wood is light and soft, and it is also used as an ornamental tree or for Christmas decorations due to the texture of its leaves and its beautiful tree shape. Studies on Abies nephrolepis include the extraction of taxifolin, diosmin, and quercetin using ultrasound (Wei M, et al., 2020. Ultrasound-Assisted Extraction of Taxifolin, Diosmin, and Quercetin from Abies nephrolepis (Trautv.) Maxim: Kinetic and Thermodynamic Characteristics. Molecules. 25:1401. doi: 10.3390/molecules25061401), the analysis of various terpenoids exhibiting cancer cell growth inhibitory activity (Ou-Yang DW et al., 2011, Miscellaneous terpenoid constituents of Abies nephrolepis and their moderate cytotoxic activities. Phytochemistry. 72: 2197-2204), and NO production inhibitory activity (Li YL et al., 2011. Phenolic compounds of Abies nephrolepis and their NO production inhibitory activities. Chem Biodivers. 8: 2299-2309), gap junction inhibitory activity in rat liver epithelial cells (Lee SJ et al., 2004. Abies nephrolepis leaf phenolics prevent the inhibition of gap junction intercellular communication by hydrogen peroxide in rat liver epithelial cells. Biofactors. 21:357-360), growth inhibitory activity of skin and lung cancer cells (Ahn C, et al.