KR-102963513-B1 - Antagonist microorganisms for inhibiting fire blight and composition for inhibiting overwater burns with the same as an active ingredient

KR102963513B1KR 102963513 B1KR102963513 B1KR 102963513B1KR-102963513-B1

Abstract

The present invention relates to an antagonistic microorganism for suppressing fire blight and a composition for preventing or suppressing fire blight containing the same as an active ingredient, and provides an antagonistic microorganism that exhibits excellent antagonistic activity against the causative agent, Erwinia amylovora . By providing an antagonistic microorganism for suppressing fire blight isolated domestically, the present invention can replace biological control agents for suppressing fire blight, which incur enormous costs due to reliance on imports.

Inventors

박덕환
최동혁
최현주
김연주
임연정
이인경

Assignees

강원대학교산학협력단

Dates

Publication Date: 20260512
Application Date: 20230208
Priority Date: 20220603

Claims (8)

Bacillus altitudinis KPB25-HP strain, a novel strain with enhanced oxidative stress resistance, deposited under accession number KACC81238BP.
In paragraph 1, The above strain is a strain that has antagonistic activity against the Erwinia amylovora strain.
A composition for preventing or suppressing fire blight of fruit trees, comprising the novel strain of claim 1 as an active ingredient.
In paragraph 3, The above-mentioned fruit tree fire blight is a composition in which the causative agent is Erwinia amylovora .
In paragraph 4, The above-mentioned fire blight is a composition induced in apples.
A biological control agent comprising a composition for preventing or suppressing fire blight of paragraph 3.
A method for preventing or suppressing fire blight, comprising the step of treating a plant with the composition for preventing or suppressing fire blight of claim 3.
In Paragraph 7, The above plant is a method that is an apple.

Description

Antagonist microorganisms for inhibiting fire blight and composition for inhibiting overwater burns containing the same as an active ingredient The present invention relates to an antagonistic microorganism for inhibiting fire blight and a composition for inhibiting fire blight containing the same as an active ingredient. Fire blight is a bacterial disease affecting certain plants, including apples, pears, and Rosaceae, caused by the pathogen * Erwinia amylovora *. It primarily occurs during the flowering season and is transmitted by bees or pathogens carried by rain. When infected, tissues such as leaves, flowers, branches, stems, and fruits turn black and gradually dry up and die. The disease poses a significant problem due to the lack of effective treatments or control agents and its rapid spread, resulting in large-scale damage. Since fire blight was first reported in the eastern United States in 1780, it has occurred in numerous countries including North America and Europe. In Korea, following the first outbreak in Anseong, Gyeonggi-do in 2015, new cases have emerged in Cheonan and Anseong in 2017, Chungju, Jecheon, Wonju, and Pyeongchang in 2018, and Paju, Yeoncheon, Icheon, and Yongin in 2019, indicating a gradual expansion of affected areas. In particular, the large-scale outbreaks in the Jecheon and Chungju regions have caused severe economic damage due to the closure of apple and pear orchards. Furthermore, as there is a possibility of the disease spreading beyond the affected areas to major fruit-growing regions such as Yeongju, Bonghwa, and Yecheon in North Gyeongsang Province, which are major apple-producing areas, and Danyang, Cheongju, and Okcheon in North Chungcheong Province, it is necessary to block new outbreaks and find alternatives to prevent further spread. Fire blight is classified into affected, buffer, and non-affected areas, and policies to prevent its spread are being implemented for each region. Although new outbreaks and spread prevention are being attempted through the application of pesticides, environmentally friendly orchards are unable to apply pesticides, so other organic materials for disease control or self-developed, unregistered treatments are often used. To solve these problems, the present invention aims to develop and provide a biological pesticide that can be used in orchards nationwide. FIG. 1 is a diagram illustrating the isolation source of 45 types of microorganisms in one embodiment of the present invention. FIG. 2 is a figure showing a strain that exhibits antagonistic activity against the fruit tree fire blight pathogen ( Erwinia amylovora ) among 45 types of microorganisms in one embodiment of the present invention. Figure 3 is a figure confirming the antagonistic activity and inhibitory size of five strains (KPB15, 21, 25, 31, 39) against the fruit tree fire blight pathogen ( Erwinia amylovora ) in one embodiment of the present invention. Figure 4 is a figure confirming the difference in activity of five strains (KPB15, 21, 25, 31, 39) according to culture medium conditions in one embodiment of the present invention. FIG. 5 is a figure confirming the motility of five strains (KPB15, 21, 25, 31, 39) in one embodiment of the present invention. FIG. 6 is a figure confirming the antagonistic activity of five strains (KPB15, 21, 25, 31, 39) against the fruit tree fire blight pathogen ( Erwinia amylovora ) in immature apples in one embodiment of the present invention. Figure 7 is a figure showing the necrotic area of disease symptoms observed on the appearance of an immature apple in one embodiment of the present invention, analyzed using Image J. FIG. 8 is a figure confirming the antagonistic activity of five strains (KPB15, 21, 25, 31, 39) against the fruit tree fire blight pathogen ( Erwinia amylovora ) in apple seedlings in one embodiment of the present invention. FIG. 9 is a figure showing the degree of disease observed in the appearance of an apple seedling in one embodiment of the present invention. FIG. 10 is a figure showing the density of the causative agent of fire blight surviving in apple seedlings in one embodiment of the present invention. FIG. 11 is a diagram showing the schematic of KPB15 in one embodiment of the present invention. FIG. 12 is a diagram showing the schematic of KPB21 in one embodiment of the present invention. FIG. 13 is a diagram showing the schematic of KPB25 in one embodiment of the present invention. FIG. 14 is a diagram showing the schematic of KPB31 in one embodiment of the present invention. FIG. 15 is a diagram showing the schematic of KPB39 in one embodiment of the present invention. FIG. 16 is a figure confirming the antagonistic activity between five strains (KPB15, 21, 25, 31, 39) in one embodiment of the present invention. FIG. 17 is a figure showing the inhibition test of H₂O₂ resistance - enhanced strains KPB25-HP (accession number: KACC81238BP) and KPB31-HP (accession number: KACC81239BP) in one embodiment of the