KR-102963603-B1 - Porous fluorescent hydrogel and manufacturing method of the same

Abstract

The present invention relates to a porous fluorescent hydrogel in which quantum dot fluorescent nanoparticles are contained within the pores of a nanoporous hydrogel, wherein the nanoporous hydrogel comprises an alginate hydrogel, an agarose hydrogel, an alginate-agarose composite hydrogel, an alginate-acrylamide composite hydrogel, an agarose-acrylamide composite hydrogel, or an alginate-agarose-acrylamide composite hydrogel. According to the present invention, since quantum dot fluorescent nanoparticles that generate fluorescence are contained within the pores of the nanoporous hydrogel, the luminosity is high and the structure is stable in the environment, and it can be utilized as a material for biomarkers or biosensors, such as DNA analysis.

Inventors

• 김창열

Assignees

• 한국세라믹기술원

Dates

Publication Date

20260513

Application Date

20221031

Claims (14)

1. Quantum dot fluorescent nanoparticles are contained within the pores of a nanoporous hydrogel, wherein the quantum dot fluorescent nanoparticles are dispersed by impregnation within the pores of the nanoporous hydrogel, thereby maintaining the fluorescent properties of the quantum dot fluorescent nanoparticles. The above-mentioned nanoporous hydrogel comprises an alginate hydrogel, an agarose hydrogel, an alginate-agarose composite hydrogel, an alginate-acrylamide composite hydrogel, an agarose-acrylamide composite hydrogel, or an alginate-agarose-acrylamide composite hydrogel. The above quantum dot fluorescent nanoparticles are composed of CdSe and have a size of 1 to 20 nm, and The above quantum dot fluorescent nanoparticles are coated with ZnS, which has a larger band gap than itself, to stabilize their fluorescence properties. By having a core-shell structure in which the ZnS forms a shell surrounding the CdSe and the quantum dot fluorescent nanoparticles form a core, the reduction of the fluorescence phenomenon caused by contaminants is prevented. A porous fluorescent hydrogel characterized in that the above quantum dot fluorescent nanoparticles are surface-modified with a surfactant to have one or more functional groups selected from the group consisting of hydroxyl groups, carboxyl groups, sulfonyl groups, and phosphate groups on their surface to exhibit hydrophilicity, and the above nanoporous hydrogel also exhibits hydrophilicity.
2. delete
3. delete
4. delete
5. delete
6. A porous fluorescent hydrogel according to claim 1, characterized in that the surfactant comprises one or more substances selected from the group consisting of sodium dodecylsulfate (SDS), cetyl trimethylammonium bromide (CTAB), cetylpyridinium chloride (CPC), phospholipid, phosphatidylserine, phosphatidylethanolamine, and phosphatidylcholine.
7. A porous fluorescent hydrogel according to claim 1, characterized in that the quantum dot fluorescent nanoparticles are contained in the nanoporous hydrogel at a molar concentration of 2 nM to 1000 mM.
8. Gelling by mixing quantum dot fluorescent nanoparticles into a solution in which a hydrogel precursor is dissolved, or by impregnating a nanoporous hydrogel into a solution in which quantum dot fluorescent nanoparticles are dispersed, The method includes the step of obtaining a porous fluorescent aerogel containing quantum dot fluorescent nanoparticles within the pores of a nanoporous hydrogel, and The above quantum dot fluorescent nanoparticles are dispersed by being impregnated into the pores of the nanoporous hydrogel, thereby maintaining the fluorescent properties of the quantum dot fluorescent nanoparticles, and The above-mentioned nanoporous hydrogel comprises an alginate hydrogel, an agarose hydrogel, an alginate-agarose composite hydrogel, an alginate-acrylamide composite hydrogel, an agarose-acrylamide composite hydrogel, or an alginate-agarose-acrylamide composite hydrogel. The above quantum dot fluorescent nanoparticles are composed of CdSe and have a size of 1 to 20 nm, and The above quantum dot fluorescent nanoparticles are coated with ZnS, which has a larger band gap than itself, to stabilize their fluorescence properties. By having a core-shell structure in which the ZnS forms a shell surrounding the CdSe and the quantum dot fluorescent nanoparticles form a core, the reduction of the fluorescence phenomenon caused by contaminants is prevented. A method for preparing a porous fluorescent hydrogel, characterized in that the above-mentioned quantum dot fluorescent nanoparticles are surface-modified with a surfactant to have one or more functional groups selected from the group consisting of hydroxyl groups, carboxyl groups, sulfonyl groups, and phosphate groups on their surface to exhibit hydrophilicity, and the above-mentioned nanoporous hydrogel also exhibits hydrophilicity.
9. delete
10. delete
11. delete
12. delete
13. A method for preparing a porous fluorescent hydrogel according to claim 8, wherein the surfactant comprises one or more substances selected from the group consisting of sodium dodecylsulfate (SDS), cetyl trimethylammonium bromide (CTAB), cetylpyridinium chloride (CPC), phospholipid, phosphatidylserine, phosphatidylethanolamine, and phosphatidylcholine.
14. A method for preparing a porous fluorescent hydrogel according to claim 8, characterized in that the quantum dot fluorescent nanoparticles are contained in the nanoporous hydrogel at a molar concentration of 2 nM to 1000 mM.

Description

Porous fluorescent hydrogel and manufacturing method of the same The present invention relates to a porous fluorescent hydrogel and a method for manufacturing the same, and more specifically, to a porous fluorescent hydrogel and a method for manufacturing the same, in which quantum dot fluorescent nanoparticles that generate fluorescence are contained within the pores of the nanoporous hydrogel, so that it has high brightness and is stable in the environment, and can be utilized as a material for biomarkers or biosensors such as DNA analysis. Quantum dots exhibit varying bandgap energies depending on the size and composition of the nanoparticles. Consequently, they absorb light to excite electrons in the conduction band, and as these excited electrons fall back to the lower valence band, fluorescence occurs. This phenomenon is utilized in applications such as fluorescent materials for displays and quantum dot light-emitting displays. Quantum dot fluorescent materials are currently in mass production as display materials and are materials that can be applied to Quantum Dots LED displays, which will replace Organic Electroluminescent Displays (OLEDs) in the future. In addition, they are receiving a lot of attention as biomarkers and sensor materials utilizing fluorescence phenomena due to their ability to control various colors. While existing biomarkers are used for observing cell staining using fluorescent proteins, research is also actively being conducted on observing the behavior of cancer cells and the like by utilizing quantum dots. Figure 1 is a schematic diagram showing quantum dot fluorescent nanoparticles dispersed in the pores inside an agarose hydrogel. Figure 2 shows sample photographs of CdSe quantum dot fluorescent nanoparticles synthesized at 260°C for different reaction times. Figure 3 is a diagram showing the fluorescence characteristics of the CdSe quantum dot fluorescent nanoparticle samples shown in Figure 2. Figure 4 is a graph showing the fluorescence characteristics of a porous fluorescent hydrogel obtained by impregnating hydrophilic CdSe quantum dot fluorescent nanoparticles into an agarose hydrogel according to Experimental Example 4. Hereinafter, preferred embodiments according to the present invention will be described in detail with reference to the attached drawings. However, the following embodiments are provided to enable those skilled in the art to fully understand the present invention and may be modified in various other forms, and the scope of the present invention is not limited to the embodiments described below. When a component is described as "comprising" another component in the detailed description of the invention or the claims, this shall not be interpreted as being limited to being composed solely of said component unless specifically stated otherwise, but shall be understood as potentially including additional components. Hereinafter, the term 'nanoporous' is used to mean porosity in which multiple pores of 1 nm or more and less than 1 µm are distributed with a size in the nanometer (nm) range. In addition, the term 'hydrogel' refers to a product in which agarose or alginate, extracted and purified from agar, a type of seaweed, is dissolved in a solvent such as water or a buffer solution and then gelled by controlling a catalyst or temperature. Furthermore, a gel hybridized by adding acrylamide to agarose, alginate, or a mixture thereof, and adding a polymerization initiator, etc., is collectively named 'composite hydrogel'. According to the definition of IUPAC (International Union of Pure and Applied Chemistry), porous materials are classified into three types based on their pore diameter (d). Pores with a diameter of less than 2 nm are classified as micropores, those in the range of 2 to 50 nm as mesopores, and finally those exceeding 50 nm as macropores. A porous fluorescent hydrogel according to a preferred embodiment of the present invention comprises quantum dot fluorescent nanoparticles contained within the pores of the nanoporous hydrogel, and the nanoporous hydrogel comprises an alginate hydrogel, an agarose hydrogel, an alginate-agarose composite hydrogel, an alginate-acrylamide composite hydrogel, an agarose-acrylamide composite hydrogel, or an alginate-agarose-acrylamide composite hydrogel. The above quantum dot fluorescent nanoparticles are composed of one or more materials selected from the group consisting of CdSe and ZnSe and may have a size of 1 to 20 nm. The above quantum dot fluorescent nanoparticles may be coated with one or more materials selected from the group consisting of CdS and ZnS, which have a larger band gap than themselves, in order to stabilize their fluorescence properties. The above quantum dot fluorescent nanoparticles exhibit hydrophilicity, and the above nanoporous hydrogel may also exhibit hydrophilicity. The above quantum dot fluorescent nanoparticles can be surface-modified with a surfactant to have one or more functional