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KR-102963773-B1 - TISSUE SPECIFIC EXPRESSION PROMOTER FROM ORYZA SATIVA LOC_OS04G46910 GENE AND USE THEREOF

KR102963773B1KR 102963773 B1KR102963773 B1KR 102963773B1KR-102963773-B1

Abstract

The present invention relates to a tissue-specific promoter, an expression vector comprising said promoter, a transformant transformed with said expression vector, and a method for producing the same. The tissue-specific promoter of the present invention can be usefully utilized to cultivate crops with increased productivity by specifically regulating gene expression necessary for improving traits of pollen and root hairs, thereby improving pollen development, flower development, and root development of major grains including rice.

Inventors

  • 김유진
  • 정기홍
  • 김의정
  • 홍우종

Assignees

  • 경희대학교 산학협력단
  • 부산대학교 산학협력단

Dates

Publication Date
20260513
Application Date
20230811

Claims (10)

  1. A mature anther or root hair-specific expression promoter consisting of the nucleotide sequence represented by SEQ ID NO. 1.
  2. delete
  3. A vector comprising a promoter according to claim 1 and an exogenous gene operably linked thereto.
  4. Transformed cells transformed by a vector according to paragraph 3.
  5. Transgenic plant body transformed by a vector according to paragraph 3 or a transformed cell according to paragraph 4.
  6. In paragraph 5, The above-described transgenic plant is characterized as being a monocotyledonous plant.
  7. In paragraph 6, The above-mentioned monocotyledonous plant is characterized by being selected from the group consisting of rice, reed, corn, grass, barley, wheat, millet, sorghum, oats, rye, sugarcane, barnyard grass, bamboo, foxtail, orchid, reed, cattail, silver grass, garlic, tulip, cymbidium, iris, lily, foxtail millet, Job's tears, barnyard grass, morning glory, tiger lily, agave, red bamboo, Chinese bamboo, wild garlic, flower beetle, broom, and titmouse.
  8. A method for transforming a plant, comprising the step of transforming the plant with a vector according to paragraph 3 or a transformed cell according to paragraph 4 to express an foreign gene in the plant.
  9. A method for preparing a rice transformant that specifically expresses mature anthers or root hairs, comprising the following steps: (1) A step of manufacturing a vector comprising a promoter consisting of a nucleotide sequence represented by SEQ ID NO. 1 and an exogenous gene operably linked thereto; (2) a step of introducing the above vector into rice; and (3) A step of selecting a rice transformant in which the above vector is introduced and the above foreign gene is expressed in a tissue-specific manner in the above rice.
  10. delete

Description

Tissue-Specific Expression Promoter Derived from Rice LOC_Os04g46910 Gene and Use Thereof The present invention relates to a tissue-specifically expressed promoter derived from the rice LOC_Os04g46910 gene (also named "OsADF6"), an expression vector comprising said promoter, a transformant transformed with said expression vector, and a method for producing the same. Rice ( Oryza sativa ) is one of the world's most important food crops, and efforts have been made to steadily increase yields over the past 20 years. However, given current population growth trends, it is now necessary to increase yields by more than 70% by 2020. Nevertheless, farmland for rice cultivation is shrinking due to industrialization in various countries, and new genetic resources used for breeding are being depleted, requiring the introduction of useful foreign genes. Fortunately, advancements in molecular biology have made it possible to isolate and manipulate useful foreign genes. By transforming these useful genes into the cells of many plants, including rice, to obtain transgenic plants with new gene combinations, they serve as valuable materials for breeding as well as for gene expression research that elucidates various biological phenomena. The production of new varieties using such transformation technology is expected to emerge as a high-value-added industry in the upcoming 21st century, and is also expected to help solve the food problem, which is highlighted as one of humanity's biggest issues, to some extent. Transformation methods known to date include obtaining many transformants in the 1980s using polyethylene glycol (PEG) and electroporation on protoplasts, the use of gene guns became widespread in the late 1990s, and recently, the use of Agrobacterium, which has been widely used in dicotyledonous plants, is also being widely utilized. Other methods include the pollen pathway and microinjection. Promoters can achieve the purpose of transformation by restricting the expression of foreign genes to the entire plant body or specific tissues, and can be classified as follows according to their function. First, there are systemic expression-inducing promoters. As a representative systemic expression-inducing promoter for plants, the promoter of the 35S RNA gene of the cauliflower mosaic virus (CaMV) is used for dicotyledonous plants. For monocotyledonous plants such as rice, rice actin and maize ubiquitin gene promoters have been mainly used, and recently, a promoter of the rice cytochrome C gene (OsOc1) has been developed by domestic researchers and is currently in use (Reference: Registration No. 10-0429335). These are already inherent in basic carriers for plant transformation to induce the expression of antibiotic or herbicide resistance genes and reporter genes used as selection markers, and they are promoters that are considered first when attempting to elucidate the function of target genes within the plant from a research perspective. Because these promoters lack tissue or organ selectivity, the transformed selection markers are expressed throughout the entire plant, resulting in delayed plant growth. Furthermore, due to the nature of the promoters, the expression of the introduced gene is limited to the target organ and cannot be fully achieved, which reduces the economic viability of the transformant. Secondly, seed-specific promoters can be cited. Representative examples include the glutelin promoter used in the development of golden rice, which is used as a promoter for major storage protein genes in rice; it is currently widely used to induce seed-specific expression in monocotyledonous plants. Promoters mainly used to induce seed-specific expression in dicotyledonous plants include the lectin promoter derived from soybeans, the napin promoter derived from Chinese cabbage, and the carrot-derived DC-3 promoter and perilla-derived oleosin promoter used in a study that enhanced vitamin E production by inducing gamma-tocopherol methyl transferase (γ-TMT) gene expression in Arabidopsis thaliana seeds (see patent application number 10-2006-0000783). The aforementioned seed-specific promoters are primarily used for purposes such as the accumulation of useful proteins and the production of useful substances in major crops where the seeds themselves are used as food or food ingredients. Third, there is a root-specific expression promoter. Arabidopsis peroxidase (prxEa) was isolated and root-specific expression was confirmed. Recently, the ibMADS gene and the glucose-inducible ADP-glucose pyrophosphatase (AGPase) gene derived from sweet potatoes were isolated, and it was confirmed that the corresponding promoter induces specific expression in roots and root-specific transient expression in carrots and radishes, leading to patent registration (Republic of Korea Registration Nos. 10-0604186 and 10-0604191). Fourth, there are other tissue-specific promoters such as leaves. These include the rbcS (ribulose bisphosphate carbox