Search

KR-102964035-B1 - SNP marker for predicting edwardsiellosis resistance of Paralichthys olivaceus and uses thereof

KR102964035B1KR 102964035 B1KR102964035 B1KR 102964035B1KR-102964035-B1

Abstract

The present invention relates to an SNP marker for predicting resistance to Edwards disease in flounder and a method for predicting resistance to Edwards disease in flounder using the same. The SNP marker of the present invention can be utilized for genomic selection of flounder resistant to Edwards disease, and can induce the development of resistant varieties and continuous production of Edwards disease.

Inventors

  • 이제희
  • 김정은
  • 완창
  • 딜리파 스리팔 리야나게
  • 이지훈
  • 김가은
  • 손한창
  • 만티갈라 아라치게 하시타 딜샨

Assignees

  • 제주대학교 산학협력단

Dates

Publication Date
20260512
Application Date
20250326

Claims (8)

  1. A polynucleotide comprising the nucleotide sequence of SEQ ID NO. 1, wherein the 36th nucleotide is G or A, and the polynucleotide comprises 10 to 100 consecutive nucleotides including the 36th nucleotide, or a polynucleotide complementary thereto, comprising an SNP (Single nucleotide polymorphism) marker. Composition for predicting resistance to Edwards disease in halibut ( Paralichthys olivaceus ).
  2. In Article 1, Predicted to be a flounder resistant to Edwards disease when the 36th base of the polynucleotide composed of the base sequence of SEQ ID NO. 1 is A, Composition for predicting resistance to Edwards disease in halibut ( Paralichthys olivaceus ).
  3. In Article 1, The above composition is a composition for predicting resistance to Edwards disease in flounder ( Paralichthys olivaceus ), comprising a preparation capable of detecting or amplifying an SNP marker for predicting resistance to Edwards disease in flounder.
  4. In Paragraph 3, A composition for predicting resistance to Edwards disease in halibut ( Paralichthys olivaceus ), characterized in that the above formulation is a primer or probe capable of detecting or amplifying the above SNP marker.
  5. A polynucleotide comprising the nucleotide sequence of SEQ ID NO. 1, wherein the 36th nucleotide is G or A, and the polynucleotide comprises 10 to 100 consecutive nucleotides including the 36th nucleotide, or a polynucleotide complementary thereto, comprising an SNP (Single nucleotide polymorphism) marker. SNP chip for predicting resistance to Edwards disease in halibut ( Paralichthys olivaceus ).
  6. A polynucleotide comprising the nucleotide sequence of SEQ ID NO. 1, wherein the 36th nucleotide is G or A, and the polynucleotide comprises 10 to 100 consecutive nucleotides including the 36th nucleotide, or a polynucleotide complementary thereto, comprising an SNP (Single nucleotide polymorphism) marker. Kit for predicting resistance to Edwards disease in halibut ( Paralichthys olivaceus ).
  7. (a) a step of amplifying or detecting polymorphic sites of SNP markers for predicting resistance to Edwards disease in flounder from DNA of a sample isolated from flounder, and (b) a step of determining the base of the polymorphic site amplified or detected in step (a), and The SNP marker of step (a) above is, A polynucleotide comprising the base sequence of SEQ ID NO. 1, wherein the 36th base of the polynucleotide is G or A, and the polynucleotide comprises 10 to 100 consecutive bases including the 36th base, or a polynucleotide complementary thereto. Method for predicting resistance to Edwards disease in halibut ( Paralichthys olivaceus ).
  8. In Article 7, Predicting that it is a flounder resistant to Edwards disease when the 36th base of the polynucleotide composed of the base sequence of SEQ ID NO. 1 is A Method for predicting resistance to Edwards disease in halibut.

Description

SNP marker for predicting edwardsiellosis resistance of Paralichthys olivaceus and uses thereof The present invention relates to an SNP marker composition for predicting resistance to Edwardsiellosis in flounder and a method for predicting resistance to Edwardsiellosis in flounder using the same. Flounder is a species primarily consumed as sashimi in live form. With the full-scale commencement of aquaculture in the 1980s, the production of farmed flounder increased rapidly, establishing it as a crucial species that currently accounts for approximately half of the domestic aquaculture production scale. However, over the past decade, the production of farmed flounder has stagnated, and damage caused by environmental factors and diseases has been increasing year by year. Edwardsiella disease, one of the causes of flounder mortality, occurs regardless of size from the fry stage to the adult stage. Although antibiotics are commonly used to treat bacterial diseases, their misuse and overuse in aquaculture farms are leading to antibiotic-resistant bacteria; this merely perpetuates a vicious cycle and does not serve as a fundamental solution. In the early 2000s, the introduction of next-generation sequencing (NGS) technology accelerated the search for large-scale genetic variations, and there is a trend of developing DNA molecular markers associated with major economic traits based on microsatellite and SNP information and utilizing them for early selection. Therefore, in this invention, significant SNP markers associated with resistance to Edwards disease are identified through a genome-wide association study (GWAS), and the SNP markers are utilized as early selection markers for resistance to Edwards disease through the analysis of the genotypes and candidate genes in which they are located. Figure 1 shows whole-genome analysis for the development of a high-quality SNP chip for flounder. Figure 2 is a graph showing the mortality rate of flounder infected with Edwards disease. Figures 3 to 53 show information on 51 SNPs for predicting resistance to Edwards disease, as well as the mean and standard deviation of survival, survival days, and survival time of flounder by genotype and box graphs. The inventors have filed a patent application for “SNP markers for predicting viral hemorrhagic sepsis virus resistance in flounder” (Korean Registered Patent No. 10-2281658), and all contents described in the said registered patent may be referenced in the present invention. The present invention identified single nucleotide polymorphisms (SNPs) capable of predicting resistance to Edwards disease through genetic trait analysis using a 70K SNP chip for flounder and genome-wide association study (GWAS) on individuals selected from Edwards disease infection experiments. In one aspect, the present invention relates to an SNP marker composition for predicting Edwards disease resistance in halibut. Specifically, the present invention comprises: a polynucleotide composed of the base sequence of SEQ ID NO. 1, wherein the 36th base is G or A, and the polynucleotide is composed of 10 to 100 consecutive bases including said 36th base, or a polynucleotide complementary thereto; a polynucleotide composed of the base sequence of SEQ ID NO. 2, wherein the 36th base is G or A, and the polynucleotide is composed of 10 to 100 consecutive bases including said 36th base, or a polynucleotide complementary thereto; a polynucleotide composed of the base sequence of SEQ ID NO. 3, wherein the 36th base is T or G, and the polynucleotide is composed of 10 to 100 consecutive bases including said 36th base, or a polynucleotide complementary thereto; a polynucleotide composed of the base sequence of SEQ ID NO. 4, wherein the 36th base is C or T, and the polynucleotide is composed of 10 to 100 consecutive bases including said 36th base, or a polynucleotide complementary thereto; A polynucleotide composed of the base sequence of SEQ ID NO. 5, wherein the 36th base is G or A, and the polynucleotide is composed of 10 to 100 consecutive bases including said 36th base, or a polynucleotide complementary thereto; a polynucleotide composed of the base sequence of SEQ ID NO. 6, wherein the 36th base is C or T, and the polynucleotide is composed of 10 to 100 consecutive bases including said 36th base, or a polynucleotide complementary thereto; a polynucleotide composed of the base sequence of SEQ ID NO. 7, wherein the 36th base is T or C, and the polynucleotide is composed of 10 to 100 consecutive bases including said 36th base, or a polynucleotide complementary thereto; a polynucleotide composed of the base sequence of SEQ ID NO. 8, wherein the 36th base is T or C, and the polynucleotide is composed of 10 to 100 consecutive bases including said 36th base, or a polynucleotide complementary thereto; A polynucleotide composed of the base sequence of SEQ ID NO. 9, wherein the 36th base is G or A, and the polynucleotide is composed of 10 to 100 consecutive bases includi