KR-102964038-B1 - SNP markers for predicting rapid growth trait in Paralichthys olivaceus under the commercial extruded pellet feeding and uses thereof

Abstract

The present invention relates to an SNP marker for predicting the growth rate of flounder and a method for predicting the growth rate of flounder using the same. The SNP marker of the present invention can be utilized for genomic selection of growth-rate flounder in a commercial compound feed environment, and can induce the development of growth-rate flounder varieties and continuous production.

Inventors

• 이제희
• 이지훈
• 정태혁
• 김정은
• 김가은
• 한차폴라 아푸하밀라게 차누카 라빈두 한차폴라
• 두와게 찬디마 기한 로드리고
• 조유환

Assignees

• 제주대학교 산학협력단

Dates

Publication Date

20260512

Application Date

20250326

Claims (11)

1. A polynucleotide comprising the nucleotide sequence of SEQ ID NO. 5, wherein the 36th nucleotide is G or A, and the polynucleotide comprises 10 to 50 consecutive nucleotides including the 36th nucleotide, or a polynucleotide complementary thereto, comprising an SNP (Single nucleotide polymorphism) marker. Composition for predicting the growth rate of the halibut ( Paralichthys olivaceus ).
2. In Article 1, The prediction of the rapid growth of the above-mentioned flounder is characterized by being performed in a commercial compound feed feeding environment, Composition for predicting the growth rate of the halibut ( Paralichthys olivaceus ).
3. In Article 1, If the 36th base of the polynucleotide composed of the base sequence of SEQ ID NO. 5 is G, it is predicted to be a fast-growing halibut, Composition for predicting the growth rate of the halibut ( Paralichthys olivaceus ).
4. In Article 1, The above composition comprises a preparation capable of detecting or amplifying SNP markers for predicting the rapid length of flounder, Composition for predicting the growth rate of the halibut ( Paralichthys olivaceus ).
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6. In Article 4, The above formulation is characterized by being a primer or probe capable of detecting or amplifying the above SNP marker. Composition for predicting the growth rate of the halibut ( Paralichthys olivaceus ).
7. A polynucleotide comprising the nucleotide sequence of SEQ ID NO. 5, wherein the 36th nucleotide is G or A, and the polynucleotide comprises 10 to 50 consecutive nucleotides including the 36th nucleotide, or a polynucleotide complementary thereto, comprising an SNP (Single nucleotide polymorphism) marker. SNP chip for predicting the kinetic length of the halibut ( Paralichthys olivaceus ).
8. A polynucleotide comprising the nucleotide sequence of SEQ ID NO. 5, wherein the 36th nucleotide is G or A, and the polynucleotide comprises 10 to 50 consecutive nucleotides including the 36th nucleotide, or a polynucleotide complementary thereto, comprising an SNP (Single nucleotide polymorphism) marker. Kit for predicting the growth length of the halibut ( Paralichthys olivaceus ).
9. (a) a step of amplifying or detecting polymorphic regions of SNP markers for predicting the rapid length of flounder from DNA of a sample isolated from flounder, and (b) a step of determining the base of the polymorphic site amplified or detected in step (a), and The SNP marker of step (a) above is, A polynucleotide comprising the base sequence of SEQ ID NO. 5, wherein the 36th base of the polynucleotide is G or A, and the polynucleotide comprises 10 to 50 consecutive bases including said 36th base, or a polynucleotide complementary thereto. Method for predicting the kinetic length of the halibut ( Paralichthys olivaceus ).
10. In Article 9, A method for predicting the growth length of a flounder ( Paralichthys olivaceus ), characterized in that the growth length prediction of the flounder is performed in a commercial compound feed feeding environment.
11. In Article 9, Predicting that it is a fast-growing halibut when the 36th base of the polynucleotide composed of the base sequence of SEQ ID NO. 5 is G, Method for predicting the kinetic length of the halibut ( Paralichthys olivaceus ).

Description

SNP markers for predicting rapid growth trait in Paralichthys olivaceus under the commercial extruded pellet feeding environment and uses thereof The present invention relates to an SNP marker composition for predicting the rapid growth of flounder in a commercial compound feed feeding environment and a method for predicting the rapid growth of flounder using the same. Flounder is a fish species primarily consumed as sashimi in the form of live fish. With the start of full-scale aquaculture in the 1980s, the production volume of farmed flounder increased rapidly, and it has established itself as a very important species that currently accounts for about half of the domestic fish farming production scale. Meanwhile, feed is one of the key factors determining production efficiency in aquaculture. Currently, over 90% of domestic land-based flounder farms utilize moisture pellets (MP), which are produced by extruding small fish such as mackerel, horse mackerel, and sardines mixed with powdered feed and nutritional supplements. However, due to declining catches and marine environmental pollution, the supply of small fish required for moisture pellet production is becoming unstable. Furthermore, moisture pellets have disadvantages, including high water content leading to significant feed loss during feeding, deterioration of the aquaculture environment which promotes pathogen proliferation, and difficulties in feed storage. To address these issues, the Ministry of Oceans and Fisheries has been pursuing a policy mandating the use of extruded pellets (EP) since 2004; however, this policy has not been implemented due to opposition from the aquaculture industry, which argues that extruded pellets result in lower growth rates and body fat percentages compared to moisture pellets. Therefore, it is crucial to develop new feed varieties that exhibit high growth rates and body fat percentages even when fed extruded pellets. To this end, we intend to identify significant single nucleotide polymorphism (SNP) markers associated with growth traits of flounder in commercial compound feed feeding environments through genome-wide association study (GWAS) and apply them to breed improvement research through genomic selection (GS) to successfully lead the transition to compound feed in the flounder aquaculture industry. Figures 1 and 2 show the results of whole-genome association analysis for the kinetics of the flounder. Figures 3 to 23 show information on 21 SNPs for the prediction of the length of flounder, information by genotype, and the mean and standard deviation of the phenotypes by genotype of the corresponding SNPs, as well as graphs. The present invention identified single nucleotide polymorphisms (SNPs) capable of predicting the growth of flounder through genetic trait analysis using a 70K SNP chip for flounder and genome-wide association study (GWAS) on selected individuals. In one aspect, the present invention relates to an SNP marker composition for predicting the rapid growth of flounder in a commercial compound feed environment. Specifically, the present invention comprises: a polynucleotide composed of the base sequence of SEQ ID NO. 1, wherein the 36th base is A or C, and the polynucleotide is composed of 10 to 50 consecutive bases including said 36th base, or a polynucleotide complementary thereto; a polynucleotide composed of the base sequence of SEQ ID NO. 2, wherein the 36th base is T or G, and the polynucleotide is composed of 10 to 50 consecutive bases including said 36th base, or a polynucleotide complementary thereto; a polynucleotide composed of the base sequence of SEQ ID NO. 3, wherein the 36th base is C or A, and the polynucleotide is composed of 10 to 50 consecutive bases including said 36th base, or a polynucleotide complementary thereto; a polynucleotide composed of the base sequence of SEQ ID NO. 4, wherein the 36th base is C or T, and the polynucleotide is composed of 10 to 50 consecutive bases including said 36th base, or a polynucleotide complementary thereto; A polynucleotide composed of the base sequence of SEQ ID NO. 5, wherein the 36th base is G or A, and the polynucleotide is composed of 10 to 50 consecutive bases including said 36th base, or a polynucleotide complementary thereto; a polynucleotide composed of the base sequence of SEQ ID NO. 6, wherein the 36th base is A or G, and the polynucleotide is composed of 10 to 50 consecutive bases including said 36th base, or a polynucleotide complementary thereto; a polynucleotide composed of the base sequence of SEQ ID NO. 7, wherein the 36th base is C or A, and the polynucleotide is composed of 10 to 50 consecutive bases including said 36th base, or a polynucleotide complementary thereto; a polynucleotide composed of the base sequence of SEQ ID NO. 8, wherein the 36th base is C or T, and the polynucleotide is composed of 10 to 50 consecutive bases including said 36th base, or a polynucleotide complementary thereto; A polynucleotide composed o