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KR-102964511-B1 - Composition for injection into the joint cavity comprising DNA fragment mixture and polyalcohol

KR102964511B1KR 102964511 B1KR102964511 B1KR 102964511B1KR-102964511-B1

Abstract

The invention relates to a composition for joint cavity repair comprising a mixture of DNA fragments and a C3 or C4 polyhydric alcohol; a method for joint repair using the same; and an application for joint repair.

Inventors

  • 전소영
  • 정다원
  • 옥슬옹

Assignees

  • 주식회사 파마리서치

Dates

Publication Date
20260513
Application Date
20250411

Claims (7)

  1. A composition for joint cavity repair comprising a mixture of DNA fragments; and a C3 or C4 polyhydric alcohol, The above DNA fragment mixture is included in an amount of 2 to 5 weight percent relative to the total composition, and The above polyhydric alcohol is included in an amount of 0.5 to 4 weight percent relative to the total composition, and A composition for restoring an articular cavity, wherein the polyhydric alcohol is one or more selected from the group consisting of glycerin, propylene glycol, and butylene glycol, and wherein the composition is injected into the articular cavity to reduce mechanical friction of the joint area through physical restoration.
  2. In claim 1, the composition is a composition for restoring the joint cavity, which is for pain relief.
  3. delete
  4. A composition for joint cavity repair according to claim 1, wherein the DNA fragment mixture and polyhydric alcohol are included in a weight ratio of 10:1:1 to 4.
  5. A composition for joint cavity repair according to claim 1, wherein the DNA fragment mixture and the polyhydric alcohol are included in a weight ratio of 5 to 1: 1 to 2.
  6. A composition for restoring an articular cavity according to claim 1, wherein the DNA fragment mixture is a polynucleotide (PN), a polydeoxyribonucleotide (PDRN), or a mixture thereof.
  7. A composition for joint cavity repair according to claim 1, wherein the DNA fragment mixture has a molecular weight of 50 to 10,000 kDa.

Description

Composition for injection into the joint cavity comprising DNA fragment mixture and polyalcohol The present invention relates to a composition for joint cavity repair comprising a mixture of DNA fragments and a C3 or C4 polyhydric alcohol; a method for joint repair using the same; and a use for joint repair. A joint is the area where bones connect; the surfaces of the two bones that make up the joint face each other, and there is a thin layer of hyaline cartilage called articular cartilage. The joint is surrounded by a connective tissue membrane that is a continuation of the periosteum, which is called the joint capsule, and its inner lumen is called the articular cavity. On the inner surface of the joint capsule is a thin membrane called the synovium, which continuously secretes a small amount of synovial fluid into the joint cavity to facilitate smooth joint movement. Osteoarthritis is a disease in which the articular cartilage covering the joint surfaces of the bones wears away, exposing the bone beneath the cartilage, and inflammation occurs in the synovial membrane surrounding the joint, resulting in pain and deformation. Conventional methods for treating damaged cartilage include debridement, bone marrow stimulating techniques, osteochondrocyte transplantation, and autologous chondrocyte transplantation. These are invasive procedures primarily performed when cartilage damage has progressed significantly; conversely, the injection of hyaluronic acid products into the joint cavity is a commonly used treatment applicable in the early stages of cartilage damage. Most invasive treatment methods present several drawbacks, including surgical resection, periosteum harvesting, complexity of use, high treatment costs, leakage of adult stem cells induced during bone marrow stimulation, and the formation of abnormal fibrotic cartilage due to hemostasis issues. Furthermore, since hyaluronic acid injections serve only as a simple lubricant, they merely facilitate smooth joint movement and have limitations in pain relief. Consequently, there is an urgent need for the development of improved biomaterials for joint cavity repair compared to conventional products. Figure 1 is a figure showing the standardized injection force evaluation results for the manufactured comparative example and example. Figures 2 to 5 show the results of the phase angle evaluation for the manufactured comparative examples and embodiments. Figure 6 is a figure showing the results of the biodegradability evaluation for the manufactured comparative example and example. Figure 7 is a figure showing the results of comparing the properties of each composition when preparing a liquid composition. Figure 8 is a figure showing the results of weight-bearing after arthritis induction. Figure 9 is a figure showing the improvement rate according to the weight-bearing results after arthritis induction. The present invention will be explained in more detail below through examples. However, the following examples are merely preferred embodiments for illustrating the present invention and are therefore not intended to limit the scope of the present invention. Meanwhile, technical matters not described in this specification can be fully understood and easily implemented by a person skilled in the art in the field of the present invention or a similar field. Preparation Example: Preparation of a biomaterial for joint cavity repair comprising a DNA fragment mixture and polyhydric alcohols A DNA fragment mixture was placed in a buffer solution and dissolved at a high temperature of 60–80°C using a heat stirrer to prepare a DNA fragment mixture solution. After adding and mixing a polyhydric alcohol to the DNA fragment mixture solution prepared at 60–80°C using a heat stirrer, the temperature of the mixed solution was lowered to reach room temperature to prepare a liquid preparation. At this time, PN (polynucleotide; manufacturer: PharmaResearch, molecular weight 50–1,500 kDa) was used as a representative example of a DNA fragment mixture, and the sample names and concentrations were prepared as shown in Tables 1 and 2 below. Sample nameDNA fragment mixture (weight%)Type of polyhydric alcohol; and content (weight%)Example 12Glycerin; 2Example 22Propylene glycol; 2Example 32Butylene glycol; 2Example 42PEG400; 2Example 52PEG4000; 2Example 62PEG6000; 2Example 72Triethylene glycol; 2 Sample nameDNA fragment mixture (weight%)Glycerin content (weight%)Comparative Example 120Example 820.5Example 921.0Example 10*22.0Example 1123.0Example 1224.0Example 1328.0Comparative Example 230Example 1430.5Example 1531.0Example 1632.0Example 1733.0Example 1834.0Example 1938.0Comparative Example 340Example 2040.5Example 2141.0Example 2242.0Example 2343.0Example 2444.0Example 2548.0Comparative Example 450Example 2650.5Example 2751.0Example 2852.0Example 2953.0Example 3054.0Example 3158.0Comparative Example 570Example 3274.0Example 3378.0Comparative Example 602.0 * Example 10 has the same