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KR-102964512-B1 - Composition for repairing tendons or ligaments comprising DNA fragment mixture and polyalcohol

KR102964512B1KR 102964512 B1KR102964512 B1KR 102964512B1KR-102964512-B1

Abstract

The invention relates to a composition for tendon or ligament repair comprising a mixture of DNA fragments and a C3 or C4 polyhydric alcohol; a method for tendon or ligament repair using the same; and an application for tendon or ligament repair.

Inventors

  • 전소영
  • 정다원
  • 옥슬옹

Assignees

  • 주식회사 파마리서치

Dates

Publication Date
20260513
Application Date
20250411

Claims (7)

  1. A composition for tendon or ligament repair comprising a DNA fragment mixture; and a C3 or C4 polyhydric alcohol, The above DNA fragment mixture is included in an amount of 2 to 5 weight percent relative to the total composition, and The above polyhydric alcohol is included in an amount of 0.5 to 4 weight percent relative to the total composition, and A composition for tendon or ligament repair, wherein the polyhydric alcohol is one or more selected from the group consisting of glycerin, propylene glycol, and butylene glycol, and wherein the composition is for tensile reinforcement of the tendon or ligament.
  2. delete
  3. A composition for tendon or ligament repair according to claim 1, wherein the DNA fragment mixture and polyhydric alcohol are included in a weight ratio of 10:1:1 to 4.
  4. A composition for tendon or ligament repair according to claim 1, wherein the DNA fragment mixture and the polyhydric alcohol are included in a weight ratio of 5 to 1: 1 to 2.
  5. A composition for repairing tendons or ligaments according to claim 1, wherein the DNA fragment mixture is a polynucleotide (PN), a polydeoxyribonucleotide (PDRN), or a mixture thereof.
  6. A composition for tendon or ligament repair according to claim 1, wherein the DNA fragment mixture has a molecular weight of 50 to 10,000 kDa.
  7. A biomaterial for tendon or ligament repair comprising the composition of any one of claims 1 and 3 to 6.

Description

Composition for repairing tendons or ligaments comprising DNA fragment mixture and polyalcohol The present invention relates to a composition for tendon or ligament repair comprising a DNA fragment mixture and a C3 or C4 polyhydric alcohol; a method for tendon or ligament repair using the same; and a use for tendon or ligament repair. Ligaments are specialized connective soft tissues that link different organs or tissues and attach bone to bone. In the latter case, ligaments are flexible enough to allow for the natural movement of bones, yet they are also strong and do not stretch, providing stability to the joints by preventing resistance to applied forces. Tendons connect muscles to bones and can withstand tension. Additionally, tendons passively regulate force during movement, providing additional stability without active work. Their elastic properties allow tendons to store and recover energy with high efficiency. In tendons and ligaments, bundles of collagen fibers are embedded within a connective matrix composed of proteoglycan components. These collagen fiber bundles provide load-carrying elements. In tendons, the collagen fibers are arranged in a nearly parallel manner, allowing them to withstand high unidirectional loads. In ligaments, the collagen fibers are arranged in a less parallel manner, thereby enabling them to withstand dominant tensile stress in one direction and smaller stress in the other. Every year, hundreds of thousands of people suffer from sprains, tears, or ruptures of ligaments, particularly in the knees, shoulders, and ankles, or injuries to tendons in the upper and lower extremities, particularly in the shoulders, knees, feet, and ankles (J Orthop Res. 2019 Sep 30;38(1):7-12). Tendons and ligaments receive a relatively insufficient supply of blood compared to other tissues in the human body, and since most tendon cells in damaged tendons no longer participate in the regeneration process, it is difficult to fully restore normal tendon function once damaged. As simple lubrication alone has limitations in improving damaged tendons or ligaments, there is an urgent need to develop improved biomaterials for tendon or ligament repair that offer efficacy beyond simple lubrication. Figure 1 is a figure showing the standardized injection force evaluation results for the manufactured comparative example and example. Figures 2 to 5 show the results of the phase angle evaluation for the manufactured comparative examples and embodiments. Figure 6 is a figure showing the results of the biodegradability evaluation for the manufactured comparative example and example. Figure 7 is a figure showing the results of comparing the properties of each composition when preparing a liquid composition. Figure 8 shows the results of tensile strength improvement by administering the composition of the present invention in a tenotomy model using rabbits. The present invention will be explained in more detail below through examples. However, the following examples are merely preferred embodiments for illustrating the present invention and are therefore not intended to limit the scope of the present invention. Meanwhile, technical matters not described in this specification can be fully understood and easily implemented by a person skilled in the art in the field of the present invention or a similar field. Preparation Example: Preparation of a biomaterial for tendon or ligament repair comprising a DNA fragment mixture and polyhydric alcohols A DNA fragment mixture was placed in a buffer solution and dissolved at a high temperature of 60–80°C using a heat stirrer to prepare a DNA fragment mixture solution. After adding and mixing a polyhydric alcohol to the DNA fragment mixture solution prepared at 60–80°C using a heat stirrer, the temperature of the mixed solution was lowered to reach room temperature to prepare a liquid preparation. At this time, PN (polynucleotide; manufacturer: PharmaResearch) was used as a representative example of a DNA fragment mixture, and the sample names and concentrations were prepared as shown in Tables 1 and 2 below. Sample nameDNA fragment mixture (weight%)Type of polyhydric alcohol; and content (weight%)Example 12Glycerin; 2Example 22Propylene glycol; 2Example 32Butylene glycol; 2Example 42PEG400; 2Example 52PEG4000; 2Example 62PEG6000; 2Example 72Triethylene glycol; 2 Sample nameDNA fragment mixture (weight%)Glycerin content (weight%)Comparative Example 120Example 820.5Example 921.0Example 10*22.0Example 1123.0Example 1224.0Example 1328.0Comparative Example 230Example 1430.5Example 1531.0Example 1632.0Example 1733.0Example 1834.0Example 1938.0Comparative Example 340Example 2040.5Example 2141.0Example 2242.0Example 2343.0Example 2444.0Example 2548.0Comparative Example 450Example 2650.5Example 2751.0Example 2852.0Example 2953.0Example 3054.0Example 3158.0Comparative Example 570Example 3274.0Example 3378.0Comparative Example 602.0 * Example 10 has the same composition and content