KR-102964788-B1 - Moraxella sp. DOX410 strain for reducing 1,4-dioxane in freshwater and wastewater and uses thereof

KR102964788B1KR 102964788 B1KR102964788 B1KR 102964788B1KR-102964788-B1

Abstract

The present invention relates to the use of a novel microbial strain of the genus Moraxella, DOX410, capable of decomposing 1,4-dioxane to purify contaminated water environments. In particular, the strain exhibits the effect of decomposing 1,4-dioxane up to a concentration of 500 ppm in freshwater environments by 100%. It also has the effect of decomposing 1,4-dioxane in actual industrial wastewater. According to the present invention, the microbial strain of the genus Moraxella, DOX410, possesses the ability to effectively remove 1,4-dioxane in freshwater environments such as chemical manufacturing, textile manufacturing, sewage treatment plants, or rivers that primarily discharge 1,4-dioxane. Therefore, it can be applied to contaminated water environments in various fields and can be utilized for environmental restoration in an eco-friendly manner.

Inventors

류병곤
강혜경
이미화

Assignees

국립낙동강생물자원관

Dates

Publication Date: 20260513
Application Date: 20231123

Claims (6)

Moraxella sp. DOX410 strain deposited as KCTC 15673BP.
A microbial preparation for reducing 1,4-dioxane, comprising the strain of claim 1 as an active ingredient.
A composition for reducing 1,4-dioxane comprising the strain of claim 1 as an active ingredient.
A method for culturing the strain of claim 1, A method of culturing in a nutrient medium under conditions of a temperature of 15℃ to 40℃, a pH of 5.5 to 9.0, and a salinity of 0% to 1%.
A method for treating fresh water or wastewater contaminated with 1,4-dioxane using the strain of claim 1, A treatment method comprising: a step of adjusting the environmental conditions of fresh water or wastewater to a temperature of 15℃ to 40℃, a pH of 5.5 to 9.0, and a salinity of 0% to 1%; and a step of introducing the strain of claim 1.
In claim 5, A treatment method further comprising the step of introducing a nutrient for growing the strain of claim 1, either before or after controlling environmental conditions, or after introducing the strain.

Description

Moraxella sp. DOX410 strain for reducing 1,4-dioxane in freshwater and wastewater and uses thereof Moraxella sp. DOX410 strain for reducing 1,4-dioxane in freshwater and wastewater and uses thereof The present invention relates to a microbial preparation and composition for reducing 1,4-dioxane, comprising a novel Moraxella sp. DOX410 strain deposited at KCTC 15673BP. The characteristics and uses of 1,4-dioxane are described in detail: (Physicochemical Characteristics) 1,4-dioxane is a colorless, flammable liquid at room temperature with the chemical formula C4H8O2 (molecular weight 88.1). It is soluble in water, solvents, and oil. Its melting point is 11.8°C, its boiling point is 101°C, and its density at 20°C is 1.03 g/mL, making it slightly heavier than water. It is stable to light and reacts with oxygen to produce peroxide. When released into the natural environment, it undergoes photochemical reactions in the atmosphere to generate OH radicals, which are incomplete substances. It is readily absorbed by soil and dissolves easily in groundwater, and it does not hydrolyze in water, resulting in a residual nature. (Main Uses) 1,4-dioxane is used as a stabilizer for chlorinated solvents and as a solvent for resins, dyes, waxes, and oils. It is also used as a solvent for adhesives, sealants, cosmetics, pharmaceuticals, rubber chemicals, and surface coatings. In Japan, it has been used as a solvent and surface treatment agent for artificial leather. The toxicity of 1,4-dioxane and domestic detection cases are described in detail: (Toxicity) The WHO announced that if an adult consumes 2 liters of water per day with a concentration of 1,4-dioxane of 50 ppb for 30 years, 1 in 100,000 people may develop cancer. The Ministry of Environment designated 1,4-dioxane as a national health protection standard item in 2014 and is managing it. (Detection Cases) 1,4-dioxane became an issue in Korea when it was detected in treated water and raw water sources in the Nakdong River basin during a survey on the presence of trace hazardous substances in tap water conducted in 2003. In January 2009, 1,4-dioxane was detected at the Duryu Water Purification Plant, one of the water treatment plants using the Nakdong River as its source, and as this became a major issue, emission regulations were implemented in earnest. Recently, even after 2020, 1,4-dioxane continues to be discharged and detected from sewage treatment plants in the Nakdong River basin as well as from various chemical and textile manufacturing industries, making it a pressing social issue. The reduction technology for 1,4-dioxane is described in detail: 1,4-dioxane present in water is difficult to remove, and reduction technologies such as UV/hydrogen peroxide, ozone/hydrogen peroxide, and Fenton oxidation, which are mainly advanced oxidation processes, are known. Although it is known that treatment is possible using microbial methods such as biological activated carbon filtration, the treatment efficiency is known to be at the level of 50–60%. Existing microorganisms capable of degrading 1,4-dioxane are described in detail: To decompose 1,4-dioxane exposed to the environment in an eco-friendly manner, biological degradation methods using microorganisms can be applied, and the microbial community of the genus Xanthobacter is a well-known representative microorganism capable of decomposing 1,4-dioxane. The background technology described in the patent document is described in detail: Patent Document 1 relates to an invention for treating wastewater containing 1,4-dioxane, and mentions Mycobacterium vaccae , Rhodococcus, Amycolata, Methylibium petroleiphilum PM1, and Azoarcus sp . EbN1 in relation to decomposition strains (see identification items [0011], [0014]). Patent Document 2 mentions Sphingomonas wittchii RW1 in relation to decomposition strains (see identification item [0004]). Patent Document 3 describes the use of transition metal catalysts and ozone instead of microorganisms (see abstract). Patent Document 4 describes pseudonocardia having 1,4-dioxane decomposition ability. A strain of the genus ( Pseudonocardia sp. ) GB7 and/or a culture thereof are described. Figure 1 is a phylogenetic tree of the Moraxella genus strain DOX, which possesses 1,4-dioxane degradation activity. Figure 2 is a electron microscope (TEM) analysis image of the Moraxella genus strain DOX410. Figure 3 is a graph showing the degradation results of 1,4-dioxane concentration according to growth and time of Moraxella strain DOX410. Figure 4 is a graph showing the degradation results of the Moraxella strain DOX410 over time according to 1,4-dioxane concentration. Figure 5 is a graph showing the growth and 1,4-dioxane degradation results of strain DOX410 in actual industrial wastewater. Figures 6 and 7 are graphs of experimental results when a nutrient component is added to improve the growth of strain DOX410 and the efficiency of 1,4-dioxane removal using said strain. Terms and words used