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KR-20260062178-A - Novel Streptomyces genus strain producing high aureothin and composition containing the same

KR20260062178AKR 20260062178 AKR20260062178 AKR 20260062178AKR-20260062178-A

Abstract

The present invention relates to a composition for controlling parasitic nematodes comprising one or more selected from the group consisting of the Streptomyces sp. AL-3 strain deposited under accession number KCTC15851BP, the lysate thereof, the extract thereof, the culture thereof, the concentrate of the culture, and the dried product of the culture; a method for preparing a Streptomyces sp. mutant strain with improved oreotin production ability; and a method for controlling parasitic nematodes using the composition. The present invention contributes to the industrial sector by developing superior actinomycetes through the selective pressure ALE method and increasing the production capacity of biologically important secondary metabolites such as antibiotics, anticancer agents, antiviral agents, immunosuppressants, and enzymes produced by actinomycetes, as well as being usefully utilized in the development and production of microbial control agents, thereby contributing to environmental conservation through the realization of eco-friendly agriculture.

Inventors

  • 손광희
  • 박동진
  • 서준형
  • 이화
  • 이병민
  • 김종훈
  • 신기선
  • 이소혜
  • 박문조

Assignees

  • 한국생명공학연구원

Dates

Publication Date
20260507
Application Date
20241025

Claims (16)

  1. Streptomyces sp. AL-3 strain deposited under accession number KCTC15851BP.
  2. In claim 1, the strain is a strain having nematicidal activity.
  3. In paragraph 2, the above nematode is a strain that is a parasitic nematode.
  4. In paragraph 2, the nematode is a strain selected from the group consisting of Bursaphelenchus spp., Caenorhabditis spp. , Meloidogyne spp . , Aphelenchodies spp. , Ditylenchus spp ., Globodera spp ., Heterodera spp. , Pratylenchus spp., Trichodorus spp. , and Xiphinema spp.
  5. In paragraph 2, the nematode is a strain of the pine nematode ( Bursaphelenchus xylophilus ) or the nematode (Caenorhabditis elegans) .
  6. In claim 1, the strain is a strain having the ability to produce oreotin.
  7. In claim 1, the strain is a strain in which the ability to produce oreotin is enhanced through adaptive evolution.
  8. A method for preparing a mutant strain of Streptomyces sp. with enhanced oreotin production ability, comprising the step of adaptively evolving a Streptomyces strain in a medium supplemented with kojic acid, sodium dehydroacetate, or a combination thereof.
  9. A method of preparation according to claim 8, wherein the Streptomyces genus strain is a Streptomyces thioluteus strain.
  10. A method of preparation according to claim 8, wherein the Streptomyces genus mutant strain is the Streptomyces sp. AL-3 strain deposited under accession number KCTC15851BP.
  11. A culture medium composition for preparing a mutant strain of Streptomyces sp. with enhanced oreotin production ability, comprising kojic acid, sodium dehydroacetate, or a combination thereof.
  12. In claim 11, the above composition is a culture medium composition further comprising a strain of the genus Streptomyces.
  13. A composition for controlling parasitic nematodes, comprising one or more selected from the group consisting of a strain of any one of claims 1 to 7, a crushed product of said strain, an extract of said strain, a culture of said strain, a concentrate of said culture, and a dried product of said culture.
  14. A method for controlling parasitic nematodes, comprising the step of treating a plant body, its seeds, its growth environment, or a parasitic nematode with the composition for controlling parasitic nematodes of claim 13.
  15. A method for controlling parasitic nematodes according to claim 14, wherein the method is characterized by directly spraying or immersing the parasitic nematode control composition onto the seeds, leaves, stems, roots, or soil adjacent thereto.
  16. A microbial preparation comprising the composition of claim 13.

Description

Novel Streptomyces genus strain producing high aureothin and composition containing the same The present invention relates to a novel Streptomyces sp. AL-3 strain that produces aureothin and the use thereof, specifically, to a Streptomyces sp. AL-3 strain having nematicidal activity; a composition comprising one or more selected from the group consisting of said strain, said lysate, said extract, said culture, said culture concentrate, and said culture dried product; a method for preparing a Streptomyces sp. mutant strain with improved aureothin production ability; a method for nematode control; a method for parasitic nematode control; and a microbial preparation. Plant-parasitic nematodes are a type of protozoan belonging to the phylum Nematoda that parasitize various parts of plants to absorb nutrients, thereby inhibiting plant growth and, in severe cases, causing the plants to wither and die. Plant-parasitic nematodes are one of the major pests causing serious damage to crops worldwide, affecting a wide variety of plants including grains, vegetables, fruits, and ornamental plants. Damage caused by plant-parasitic nematodes is estimated to result in a 12% annual decrease in global agricultural productivity and financial losses of approximately $130 billion. In Korea, damage caused by plant-parasitic nematodes has increased by an average of 10% annually since 1990, manifesting as widespread agricultural damage by 2008. Parasitic nematodes not only harm the productivity of economic crops such as vegetables and medicinal plants but also damage trees crucial for maintaining the environmental ecosystem, such as pine trees. Among them, pine wilt disease caused by the pine nematode ( *Bursaphelenchus xylophilus *) is a plant disease classified as International Quarantine No. 1 because it causes serious damage to conifers such as pine trees. It was first reported in Japan in the 1900s and causes fatal damage to susceptible Pinus species in various countries including Korea, Japan, Taiwan, China, and Portugal. Furthermore, the pine wilt nematode is a pathogen that multiplies in many host plants, including pine trees, firs ( *Abies *), spruce (* Pea* ), Douglas fir ( *Pseudotsuga *), larch (* Lalix *), deciduous larch (* Cedrus* ), and pine trees ( *Tsuga * spp.). It blocks the tracheids of host plants to prevent water from rising and secretes cellulase to destroy tissues, causing the host plants to wither and die. However, it lacks the ability to move between hosts on its own and infects host plants through insect vectors such as the long-horned beetle ( *Monochamus alternatus *). To control the pine nematode, methods such as trunk injection of nematicides like abamectin or emamectin benzoate or ground spraying of substances like fosthiazate, as well as various highly toxic chemical insecticides targeting vectors, have been primarily used. Direct control of the pine nematode is mainly used because it is more effective than control targeting vectors. However, there is a high demand for new substances due to adverse effects on humans and animals, such as wood fatigue caused by repeated trunk injections and safety concerns arising from increased toxicity. To address these issues, research on pine nematode control substances that can replace chemical pesticides is currently being actively conducted worldwide, and there is a need for a technological breakthrough. Microorganisms that produce nematicidal active substances are one of the major research methods, and to date, there is no notable control technology of microbial origin other than Abamectin (Published Patent KR 10-2011-0042233 A (2011.04.25) etc.), which is the most widely used pesticide in the pine nematode control market. Meanwhile, among microorganisms, actinomycetes are Gram-positive bacteria that produce abundant amounts of biologically important secondary metabolites, such as antibiotics, anticancer agents, antivirals, immunosuppressants, and enzymes. They are attracting attention as industrially and biologically important microorganisms with high utility value because they help maintain the ecological equilibrium of the soil by cooperating with nutrient cycling without polluting the environment. Accordingly, various approaches, including molecular biology and synthetic biology, have recently been pursued to utilize actinomycetes industrially. However, due to their very complex and strong cell walls and base composition with high G-C content, there have been difficulties in genetic analysis and manipulation. Figure 1 is a figure showing a comparison of changes in community morphology through adaptive evolution between the Streptomyces thioluteus DSM40027 strain and the Streptomyces AL-3 strain (left), and an HPLC profile confirming a comparison of oreotin content (right). Figure 2 is a phylogenetic schematic diagram showing the 16S rRNA sequence of the Streptomyces AL-3 strain compared with that of Streptomyces species. Figure 3 is a graph evaluating the