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KR-20260062240-A - NOVEL STRAIN BURKHOLDERIA AND USE THEREOF

KR20260062240AKR 20260062240 AKR20260062240 AKR 20260062240AKR-20260062240-A

Abstract

The present invention relates to a novel strain of Bulkholderia cepacia JK6 (KCTC 16020BP) identified from the roots of a black locust tree. The Bulkholderia cepacia JK6 strain according to the present invention exhibited antimicrobial activity against Fusarium oxysporum strains. In addition, the Bulkholderia cepacia JK6 strain exhibited phosphate solubilizing ability and siderophore production ability. Therefore, the Bulkholderia cepacia JK6 strain according to the present invention can be usefully utilized in the future as an antagonistic microorganism against Fusarium oxysporum strains that cause various plant fungal diseases, including banana Panama disease, and as a microorganism for promoting plant growth.

Inventors

  • 김인성
  • 엄완숙

Assignees

  • 농업회사법인 주식회사 오브제바이오

Dates

Publication Date
20260507
Application Date
20241028

Claims (12)

  1. Burkholderia cepacia JK6 strain (accession number KCTC 16020BP).
  2. In paragraph 1, The strain above is a strain comprising the 16s rRNA gene sequence of SEQ ID NO. 1.
  3. In paragraph 1, The strain above is a strain having antifungal activity or plant growth-promoting activity.
  4. Culture of Bulkholderia cepacia JK6 strain (accession number KCTC 16020BP).
  5. A composition for controlling plant pathogenic fungi comprising the Bulkholderia cepacia JK6 strain (accession number KCTC 16020BP) or a culture thereof as an active ingredient.
  6. In paragraph 5, A composition for controlling plant pathogenic fungi, wherein the above-mentioned plant pathogenic fungus is a strain of Fusarium oxysporum.
  7. In paragraph 6, A composition for controlling plant pathogenic fungi, wherein the above-mentioned Fusarium oxysporum strain is one or more selected from the group consisting of F. oxysporum f.sp. cubense, F. oxysporum f.sp. radicis-lycopersici, F. oxysporum f.sp. gladioli, F. oxysporum f.sp. lactucae, F. oxysporum f.sp. cucumerinum, and F. oxysporum f.sp. melonis.
  8. A method for controlling plant pathogenic fungi comprising the step of applying the composition for controlling plant pathogenic fungi of claim 5 to a plant or soil adjacent to the plant.
  9. A composition for promoting plant growth comprising the Bulkholderia cepacia JK6 strain (accession number KCTC 16020BP) or a culture thereof as an active ingredient.
  10. A method for promoting plant growth comprising the step of treating a plant or soil adjacent to a plant with the plant growth-promoting composition of claim 9.
  11. A kit for controlling plant pathogens comprising the Burkholderia cepacia JK6 strain (accession number KCTC 16020BP) or a culture thereof.
  12. A kit for promoting plant growth comprising the Burkholderia cepacia JK6 strain (accession number KCTC 16020BP) or a culture thereof.

Description

Novel STRAIN BURKHOLDERIA AND USE THEREOF The present invention relates to a novel Bulkholderia strain and a composition for controlling plant pathogenic fungi and a composition for promoting plant growth containing the same as an active ingredient. Plant pathogenic fungi cause damage to many crops, significantly reducing their productivity. To protect crops from these fungi, chemical pesticides have been consistently used worldwide. However, the misuse and overuse of chemical pesticides have raised various issues, including soil, water, and agricultural product contamination, toxicity, and ecosystem disruption. Consequently, research aimed at developing biopesticides to replace these chemical pesticides is actively underway globally. In line with this, numerous microorganisms that engage in beneficial interactions with plants are being discovered, and efforts to utilize them as biofertilizers or biocontrol agents are continuing. As described above, microorganisms that exert beneficial effects on plant growth in the rhizosphere are called plant growth-promoting rhizobacteria (PGPR). Numerous studies have reported that these microorganisms enhance plant growth and vitality, and increase yields. These microorganisms are known to aid plant growth by decomposing organic matter in the soil or by converting nutrients that are difficult for plants to utilize into forms that are easily absorbed and utilized. Furthermore, they protect plants by exhibiting antagonistic effects against harmful bacteria that cause plant diseases (Olanrewaju et al. (2017) Mechanisms of action of plant growth promoting bacteria. World J Microbiol Biotechnol 33:197). Among these, there are products developed to utilize bacteria that exhibit antifungal activity and promote plant growth in agriculture, but most are limited to Bacillus, Pseudomonas, and Streptomyces. In Korea, although Enterobacter, which promotes plant growth, was disclosed in Korean Registered Patent No. 10-1611537, the development of effective, low-toxicity antifungal agents for clinical use and domestic microbial pesticides remains insufficient. Therefore, there is a need for more active research on effective microorganisms that can be used as biopesticides to control plant pathogenic fungi. Figure 1 is a diagram showing the results of culturing (2 days) microorganisms isolated from the roots of an acacia tree. Figure 2 is a diagram showing the results of confirming the antimicrobial activity of a microorganism isolated from the roots of a black locust tree against Fusarium oxysporum f.sp. cubense tropical race 4 (FocTR4). Figures 3a and 3b show the results of blast analysis of the 16s rRNA gene sequence of Gj-5 among the 13 microorganisms isolated from the roots of acacia trees. Figures 4a and 4b show the results of blast analysis of the 16s rRNA gene sequence of Gj-8 among the 13 types of microorganisms isolated from the roots of acacia trees. Figures 5a and 5b show the results of blast analysis of the 16s rRNA gene sequences of 11 types of microorganisms (13 types) isolated from the roots of acacia trees. Figures 6a to 6d show the results of multiple sequence alignment analysis of the 16S rRNA gene sequences of 11 microorganisms predicted to be Burkholderia cepacia species among microorganisms isolated from the roots of black locust trees. Figure 7 is a diagram showing phylogenetic groups classified based on 16s rRNA gene sequences for 11 species of microorganisms predicted to be Bulkholderia cepacia species among microorganisms isolated from the roots of black locust trees. Figure 8 is a diagram showing the results of comparing the colony colors of Bulkholderia cepacia JK6 strain (KCTC 16020BP) and Bulkholderia cepacia JK11 strain (KCTC 14199BP), which are strains of the same genus. Figure 9 is a figure showing the results of comparing the motility and antibacterial activity against Escherichia coli of the Bulkholderia cepacia JK6 strain (KCTC 16020BP) and the genus Bulkholderia cepacia JK11 strain (KCTC 14199BP). Figure 10 is a diagram showing the phylogenetic tree of the Bulkholderia cepacia JK6 strain and the Bulkholderia cepacia JK11 strain. Figure 11 is a figure showing the results of confirming the antibacterial activity of the Bulkholderia cepacia JK6 strain according to the present invention against the F. oxysporum f.sp. lactucae strain. Figure 12 is a figure showing the results of confirming the antibacterial activity of the Bulkholderia cepacia JK6 strain according to the present invention against the F. oxysporum f.sp. lagenariae strain. Figure 13 is a figure showing the results of confirming the antibacterial activity of the Bulkholderia cepacia JK6 strain according to the present invention against the F. oxysporum f.sp. cucumerinum strain. Figure 14 is a figure showing the results of confirming the antibacterial activity of the Bulkholderia cepacia JK6 strain according to the present invention against the F. oxysporum f.sp. cubense strain. Figure 15 is