KR-20260062262-A - Skin whitening composition using exosome of lactic acid bacteria strain Leuconostoc mesenteroides DB1 isolated from Camellia japonica flower

Abstract

The skin whitening composition of the present invention contains exosomes derived from the Leuconostoc mesenteroides DB1 strain deposited under accession number KACC 92611P as an active ingredient.

Inventors

• 지원재
• 김다솜

Assignees

• 대한민국(기후에너지환경부 국립생물자원관장)

Dates

Publication Date

20260507

Application Date

20241028

Claims (6)

1. A skin whitening composition containing exosomes derived from the Leuconostoc mesenteroides DB1 strain deposited under accession number KACC 92611P as an active ingredient.
2. A skin whitening composition according to claim 1, wherein the exosomes are obtained by purifying a culture medium obtained by culturing the strain in MRS medium.
3. A skin whitening composition according to claim 1, wherein the exosomes are obtained by purifying a culture medium obtained by shaking culture the strain in a liquid medium at 25 to 35°C for 15 to 20 hours.
4. A step of obtaining a culture medium by culturing the Leuconostoc mesenteroides DB1 strain deposited under accession number KACC 92611P in a liquid medium; and Step of purifying exosomes derived from the strain from the above culture medium A method for producing a skin whitening active substance including
5. A method for producing a skin whitening active substance, wherein, in paragraph 4, the liquid medium is an MRS medium.
6. A method for producing a skin whitening active substance according to claim 4, wherein the culture is a shaking culture at 25 to 35°C for 15 to 20 hours.

Description

Skin whitening composition using exosome of lactic acid bacteria strain Leuconostoc mesenteroides DB1 isolated from Camellia japonica flower The present invention relates to a skin whitening composition using exosomes of the lactic acid bacteria strain Leuconostoc mesenteroides DB1 isolated from camellia flowers. More specifically, the present invention relates to a skin whitening composition capable of providing an excellent skin whitening effect by utilizing a novel lactic acid bacteria strain isolated from camellia flowers, which are a biological resource of Korea, and also relates to a method capable of efficiently producing a skin whitening active substance. Active research is being conducted to identify and utilize commercially and/or industrially useful microorganisms among the countless microorganisms in nature. Although various useful microorganisms have been discovered and utilized to date, it is still necessary to expand the pool of available useful microorganisms by newly discovering them, and at the same time, it is also necessary to discover new applications that leverage the characteristics of microorganisms. Meanwhile, skin whitening, which involves making the skin beautiful and white, is a topic of great interest in the field of functional cosmetics, and accordingly, the discovery of substances capable of exhibiting such whitening effects is actively underway. Generally, inhibiting the formation of melanin is crucial for achieving a whitening effect. Therefore, substances with excellent whitening effects can be identified based on their melanin-inhibiting activity, and the whitening effect of candidate substances can be confirmed by investigating the influence on the activity and/or expression of tyrosinase, an enzyme involved in the early stages of melanin production, as a related factor. The inventors of the present invention sought to discover useful microbial resources from various natural samples in Korea and to develop a method to utilize these microbial resources more efficiently through further research. In particular, they sought to develop a method that can be utilized in the field of functional cosmetics by studying the whitening-related activities mentioned above. Figure 1 shows the 16S rRNA gene sequence of the Leuconostoc mesenteroides DB1 strain of the present invention. Figure 2 shows the Nanoparticle Tracking Analysis (NTA) results of exosomes purified from the DB1 strain of the present invention. Figure 3 shows the results of a cytotoxicity test of exosomes purified from the DB1 strain of the present invention. Figure 4 shows the experimental results of the melanin synthesis inhibitory activity of exosomes purified from the DB1 strain of the present invention. Figure 5 shows the experimental results of the tyrosinase activity inhibitory activity of exosomes purified from the DB1 strain of the present invention. Figure 6 shows the experimental results of the MITF and TRP-2 expression inhibitory activity of exosomes purified from the DB1 strain of the present invention. The skin whitening composition of the present invention is characterized by containing exosomes derived from the strain of the present invention, namely exosomes derived from the DB1 strain, as an active ingredient. The strain of the present invention is a novel lactic acid bacterium strain belonging to the species * mesenteroides * of the genus * Leuconostoc *, with the strain name DB1. It was isolated from the flowers of * Camellia japonica L.* and deposited at the Korean Agricultural Culture Collection (KACC) of the National Institute of Agricultural Sciences, Rural Development Administration under accession number KACC 92611P. The strain of the present invention has the 16S rRNA gene sequence of SEQ ID NO. 1 (Fig. 1). The strain of the present invention is a different strain that differs from the existing strain of similar origin disclosed in Korean Registered Patent No. 10-2567092, Leuconostoc mesenteroides DB3 strain in terms of citric acid utilization, acetoin production, D-mannitol fermentation/oxidation, esterase lipase (C8) activity, and valine arylamidase activity. The strain of the present invention can be cultured according to conventional culture methods used for culturing lactic acid bacteria, particularly lactic acid bacteria of the genus Leuconostoc, and particularly lactic acid bacteria of the species Leuconostoc mesenteroides. For example, it can be cultured using MRS medium at a temperature of 20 to 40°C. The composition of MRS medium is well known to those skilled in the art. For more efficient growth, the temperature condition is preferably 25 to 35°C, more preferably 28 to 32°C, and even more preferably 29 to 31°C. The exosomes of the present invention, namely the exosomes derived from the DB1 strain of the present invention, can be obtained according to conventional methods used to obtain exosomes from cells. For example, they can be obtained by culturing the DB1 strain to obtain a cul