KR-20260062560-A - COSMETIC COMPOSITION COMPRISING OF ANDROGRAPHOLIDE AND EXTRACTS OF BIDENS PILOSA L.

Abstract

The present invention relates to a cosmetic composition comprising andrographolide and Bidens pilosa extract. The cosmetic composition of the present invention, when using andrographolide and Bidens pilosa extract together, exhibited superior effects in improving wrinkles, skin irritation, elasticity, inflammation, and antioxidant properties compared to when each was used alone.

Inventors

• 예상현
• 전승현

Assignees

• 주식회사 엘지생활건강

Dates

Publication Date

20260507

Application Date

20241029

Claims (7)

1. A cosmetic composition comprising andrographolide and Bidens Pilosa L. extract.
2. In paragraph 1, The above composition is a cosmetic composition for improving wrinkles, improving skin irritation, enhancing skin elasticity, anti-inflammatory, or antioxidant purposes.
3. In paragraph 1, The above-mentioned Ulsan devil's needle extract is a cosmetic composition extracted by one or more methods selected from the group consisting of supercritical extraction, hot water extraction, cold maceration extraction, ultrasonic extraction, and reflux cooling extraction.
4. In paragraph 1, A cosmetic composition in which the above andrographolide is included in an amount of 0.0001 to 10 weight% relative to the weight of the total composition.
5. In paragraph 1, A cosmetic composition in which the above-mentioned Ulsan devil's needle extract is included in an amount of 0.0001 to 10 weight% relative to the weight of the total composition.
6. In paragraph 1, The above cosmetic composition comprises andrographolide and Bidens pilosa extract in a ratio of 1:2 to 1:12.
7. A cosmetic composition according to claim 1, wherein the andrographolide and the extract of Bidens pilosa in the cosmetic composition are contained within liposomes.

Description

Cosmetic composition for skin improvement comprising andrographolide and extracts of Bidens pilosa L. The present invention relates to a cosmetic composition for skin improvement comprising andrographolide and Devil's Needle extract, and a method for preparing the same. Skin aging includes natural aging caused by genetic factors and external aging caused by the living environment. The biggest external cause of skin aging is ultraviolet (UV) radiation. UV rays penetrate the skin, inducing inflammatory and oxidative reactions that cause skin damage, and also directly affect DNA, disrupting skin homeostasis. UV rays promote the expression of Matrix Metalloproteinase-1 (MMP-1), an enzyme that breaks down Collagen Type 1 and contributes to the formation of skin wrinkles. Meanwhile, retinol is a type of Vitamin A known for its excellent anti-aging effects and is widely used in anti-wrinkle cosmetics. Retinol is known to act on skin fibroblasts to inhibit MMP-1 enzyme expression and increase collagen type-1 synthesis, thereby improving skin wrinkles. However, retinol has the disadvantage of being difficult to use during the day due to its susceptibility to skin irritation and photodegradation. To solve these problems, there is a need to develop effective materials that exhibit excellent skin improvement like retinol while being non-irritating. Figure 1 is a graph showing the comparison of the expression levels of the RXRA (Retinoid X receptor alpha) gene after treatment with andrographolide and Ulsan devil's needle extract. Figure 2 is a graph showing the collagen protein secretion amount (%) measured and compared after treatment with andrographolide and Ulsan devil's needle extract. Figure 3 is a graph showing the comparison of MMP-1 protein secretion (%) measured after treatment with andrographolide and Ulsan devil's needle extract. Figure 4 is a figure showing the comparison of fibrillin-1 protein expression after treatment with andrographolide and Ulsan devil's needle extract. Figure 5 is a graph showing the area (%) of fibrillin-1 protein measured and compared after treatment with andrographolide and Devil's Needle extract. Figure 6 is a graph showing the IL-1a protein secretion amount (%) measured and compared after treatment with andrographolide and Ulsan devil's needle extract. Figure 7 is a graph showing the cell viability (%) reduced by hydrogen peroxide after treatment with andrographolide and Bidens pilosa extract. The advantages and features of the present invention and the methods for achieving them will become clear by referring to the embodiments described in detail below. However, the present invention is not limited to the embodiments disclosed below but may be implemented in various different forms; these embodiments are provided merely to ensure that the disclosure of the present invention is complete and to fully inform those skilled in the art of the scope of the invention, and the present invention is defined only by the scope of the claims. The raw materials used in the present invention were purchased from raw material suppliers that are typically available for purchase. Preparation Example 1. Preparation of andrographolide and Bidens pilosa extract I purchased and used both Ulsan devil's needle extract (Revinage®, Chemyunion, Brazil) and andrographolide (AlphaTriptec). Preparation Example 2. Liposomalization of andrographolide and Bidens pilosa extract Dipropylene glycol, hydrogenated lecithin, cholesterol, stearic acid, sorbitan stearate, andrographolide, and Bidens pilosa extract were mixed and dissolved at 350 rpm at 80 ℃ as shown in the ingredients of Table 1 below. Then, purified water at 80 ℃ was added and mixed at 350 rpm for 30 minutes, followed by natural cooling at room temperature to prepare liposomes containing andrographolide and Bidens pilosa extract. ingredientContent (Unit: weight%)Dipropylene glycol33.33Hydrogenated lecithin2cholesterol0.1Stearic acid0.1Sorbitan stearate0.1Andrographolide0.001Ulsan Devil's Needle Extract0.1purified waterRemaining amount Examples In this example, the efficacy of andrographolide and Bidens pilosa extract in promoting retinoid X receptor alpha gene expression (Example 1), collagen synthesis (Example 2-1), MMP-1 expression reduction (Example 2-2), Fibrillin-1 expression promotion (Example 2-3), skin inflammation improvement (Example 3-1), and inflammation relief and antioxidant efficacy was confirmed. Example 1. Evaluation of Retinoid X Receptor Alpha Gene Expression of Andrographolide and Bidens pilosa Extract To measure the expression level of RXRA, a retinoid X receptor gene, in the cell nucleus, human dermal fibroblasts were seeded into 6-well plates at a density of 3 x 10^5 cells/well and cultured overnight in a CO2 incubator. After 24 hours, andrographolide and Bidens pilosa extracts were mixed into DMEM media (Dulbecco Modified Eagle Medium) at the concentrations shown in Table 1 below and then treated with the cells. After 24 hours, R