KR-20260062724-A - Peptone derived from salmon by-product and composition of medium for culturing microorganisms containing the same

Abstract

The present invention relates to a method for producing peptone derived from salmon by-products and a culture medium composition for microorganisms comprising the same, the method comprising the steps of: heating salmon by-products to pulverize muscle protein separated from bone; mixing water with the muscle protein powder to prepare a mixture; adding a hydrolytic enzyme to the mixture to hydrolyze it; and drying the hydrolyzed mixture to obtain peptone powder. In addition, the present invention provides a peptone derived from salmon by-products and a microbial culture medium composition containing the same, which has anti-aging, reverse aging, and antioxidant effects, and can be utilized in the development of health functional food, cosmetic, and pharmaceutical industries, and can help reduce environmental pollution because it uses fish waste.

Inventors

• 오철홍
• 박건후
• 김영화
• 이수진
• 스비니 딜리파 마라싱헤
• 웨르라개 민타리 판타르
• 조은영
• 임정현
• 이영득

Assignees

• 한국해양과학기술원

Dates

Publication Date

20260507

Application Date

20241029

Claims (8)

1. A step of heating salmon by-products to powderize muscle protein separated from the bones; A step of preparing a mixture by mixing water with the above muscle protein powder; A step of hydrolyzing the above mixture by adding a hydrolytic enzyme; and A method for producing peptone derived from salmon by-products, comprising the step of drying the hydrolyzed mixture to obtain peptone powder.
2. In paragraph 1, The above hydrolytic enzyme is, A method for producing peptone derived from salmon by-products, characterized by being one or more selected from the group consisting of papain, protamax, trypsin, bromelain, alcalase, and pronase.
3. In paragraph 1, The above hydrolysis step is, A method for producing peptone derived from salmon by-products, characterized by adjusting the above mixture to a pH of 6 to 8, adding the above hydrolytic enzyme, and treating at 45 to 60 ℃ for 5 to 7 hours.
4. In paragraph 1, The above hydrolysis step is, A method for producing peptone derived from salmon by-products, further comprising the step of inactivating the hydrolytic enzyme at 90 to 100 ℃ for 5 to 15 minutes after the above hydrolysis.
5. A microbial culture medium composition comprising peptone produced by the method for producing peptone derived from salmon by-products of claim 1.
6. In paragraph 5, The above peptone is, A microbial culture medium composition characterized by being a nitrogen source for the microbial culture medium.
7. In paragraph 5, The above peptone is, A microbial culture medium composition characterized by exhibiting anti-aging, reverse aging, and antioxidant activities.
8. In paragraph 5, The above peptone is, A microbial culture medium composition characterized by improving the productivity of genetically modified proteins.

Description

Peptone derived from salmon by-product and composition of medium for culturing microorganisms containing the same The present invention relates to peptone derived from salmon byproducts and a microbial culture medium composition containing the same. More specifically, it relates to a microbial culture medium composition that uses peptone extracted from salmon head muscle protein, which is fish waste, to improve the production of recombinant proteins and has excellent anti-aging, reverse aging, and antioxidant activities. As global fisheries production has increased significantly due to population growth and advancements in fishing technology, fish waste has also risen substantially. This global increase in fish waste is causing massive environmental problems and economic losses. Therefore, there is a need to develop technologies for the efficient processing and recycling of fish waste. Maximizing the utilization of currently underutilized, low-value fish residues—such as skin, scales, fins, bones, flesh, heads, and internal organs—can produce high-demand compounds like collagen, gelatin, fish oil, omega-3 fatty acids, bioactive peptides, protein hydrolysates, and enzymes. These high-value compounds are utilized in various industries, including food processing, health functional foods, pharmaceuticals, cosmeceuticals, and biotechnology. Therefore, converting nutrient-rich fish waste, which is often discarded, into high-value-added products through sustainable and economically viable technologies helps maximize the efficient utilization of fish resources. Meanwhile, peptone serves as an essential nutrient source for microbial and cell culture, playing a significant role in various industries such as life science research, pharmaceuticals, the production of genetically modified drugs, and the fermentation industry. In the food industry, it enhances flavor and nutrition, while in cosmetics, it aids in skincare products by promoting cell regeneration and hydration. In the biopharmaceutical industry, peptone is used as a primary ingredient to increase the productivity of protein drugs and antibiotics. In particular, the production of biopharmaceuticals is one of the most promising achievements in the field of biotechnology, and research to increase productivity plays an important role as demand rises due to advancements in biotechnology and human aging. As part of efforts to increase productivity, the inventors developed peptone using salmon heads. Additionally, since it has been reported that some fish protein hydrolysates exhibit anti-aging effects, the inventors completed the present invention by using peptone, a type of protein hydrolysate, to confirm antioxidant activity, anti-aging effects, and anti-aging effects that revert old cells back to young cells. FIG. 1 is a protein electrophoresis diagram comparing the hydrolysis efficiency of salmon head-derived muscle protein prepared according to one embodiment of the present invention by various hydrolytic enzymes. ((a) bromelain, (b) papain, (c) pronase, (d) protamax, (e) trypsin, (f) alkalase) Figure 2 is a diagram showing the recovery rate of water-soluble protein after protein hydrolysis of salmon head-derived protein prepared according to one embodiment of the present invention, categorized by type of hydrolytic enzyme. Figure 3 is a diagram analyzing the active oxygen scavenging efficacy of salmon head peptone prepared according to one embodiment of the present invention. Figure 4 is a diagram analyzing the efficacy of inhibiting HDF cell aging caused by LPS according to treatment with salmon head peptone prepared according to one embodiment of the present invention. Figure 5 is a diagram analyzing the efficacy of inhibiting HDF cell aging caused by H₂O₂ according to treatment with salmon head peptone prepared according to one embodiment of the present invention. Figure 6 is a diagram showing the change in HDF cell size after treating aged HDF cells with salmon head peptone, PBS control, plant peptone, and animal peptone prepared according to one embodiment of the present invention. Figure 7 is a diagram showing the analysis of changes in p21 mRNA, cell viability by the MTT method, and cell viability by the cell counting method after treating aged HDF cells with salmon head peptone, PBS control, plant peptone, and animal peptone prepared according to one embodiment of the present invention. Figure 8 is a diagram showing the changes in expression of IL1β, SAA1, SLIT2, KLF4, SOX2, and OCT4 mRNA after treating aged HDF cells with salmon head peptone prepared according to one embodiment of the present invention, PBS control, plant-based peptone, and animal-based peptone. FIG. 9 is a diagram comparing the recombinant protein productivity of microbial culture media containing salmon head peptone, a PBS control, plant peptone, and animal peptone, respectively, prepared according to one embodiment of the present invention. The terms used in this specification wi