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KR-20260062960-A - Plant cultivation methods, uses, and products

KR20260062960AKR 20260062960 AKR20260062960 AKR 20260062960AKR-20260062960-A

Abstract

A method for cultivating plants, uses, and products are provided. The method for cultivating plants comprises the step of introducing an induction medium to an initial plant and then culturing the plant to obtain a target plant with optimized traits compared to the initial plant, without containing the induction medium. The induction medium can induce demethylation of 6-methylated bases in plant RNA. The method for cultivating plants can be used to obtain a plant having optimized traits that does not contain the induction medium introduced initially.

Inventors

  • 지아 구이팡
  • 리 지
  • 리 웨이펑

Assignees

  • 에피플랜트 컴퍼니 리미티드

Dates

Publication Date
20260507
Application Date
20240628
Priority Date
20230831

Claims (20)

  1. A method for culturing plants, characterized by introducing an induction medium into an initial plant and obtaining a target plant having optimized traits compared to the initial plant without containing the induction medium through cultivation; wherein the induction medium is capable of inducing demethylation of 6-methylated bases in plant RNA.
  2. A method according to claim 1, wherein the target plant has at least one trait with an optimization degree of Y compared to the initial plant.
  3. A method according to claim 1, wherein the induction medium is temporarily introduced into an initial plant to directly obtain a target plant having optimized traits compared to the initial plant without containing the induction medium.
  4. A method according to claim 1, wherein the induction medium is temporarily introduced into the protoplast of the initial plant.
  5. A method according to claim 1, wherein the induction medium is introduced into the initial plant to obtain an intermediate plant, and the intermediate plant undergoes subsequent cultivation to obtain a target plant having optimized traits compared to the initial plant without containing the induction medium.
  6. In paragraph 5, the method wherein the intermediate plant contains the induction medium.
  7. In paragraph 5, the method wherein the target plant has at least one trait with an optimization degree of Y compared to the initial plant is optimized.
  8. In claim 7, the method wherein the intermediate plant has at least one trait with an optimization degree of X compared to the initial plant.
  9. A method according to either claim 2 or 7, wherein Y is the growth rate relative to the initial plant of the same period, and Y≥0.20; Y≥0.50; Y≥1.00; preferably Y≥2.00; preferably Y≥3.00; preferably Y≥4.00.
  10. A method according to claim 1, wherein the trait optimization includes an increase in yield.
  11. A method according to claim 1, wherein the trait optimization includes increasing the yield of plant organs.
  12. A method according to claim 1, wherein the trait optimization comprises at least one increase in the volume, quantity, weight, or tillering number of plant organs.
  13. A method according to claim 1, wherein the induction medium can induce demethylation of 6-methyladenine in plant RNA.
  14. A method according to claim 1, wherein the induction medium is introduced to the initial plant in an excess amount.
  15. The method according to claim 1, wherein the induction medium is a nucleic acid molecule and/or polypeptide, its homologue, its functional variant, or conjugate.
  16. A method according to claim 1, wherein, where the induction medium is a nucleic acid molecule, a homologue thereof, a functional variant thereof, or a conjugate thereof, the target plant does not contain the induction medium and does not contain a polypeptide obtained from the expression of the induction medium; and where the induction medium is a polypeptide, a homologue thereof, a functional variant thereof, or a conjugate thereof, the target plant does not contain the induction medium.
  17. In claim 1, the induction medium is selected from the m6A demethylase of RNA and the nucleic acid encoding it.
  18. A method according to claim 1, wherein the induction medium is selected from at least one of an FTO nucleic acid molecule and/or polypeptide, its homologues, its functional variants, or conjugates.
  19. In claim 1, the FTO in the induction medium is derived from vertebrates, invertebrates, birds, their orthologs, or paralogs.
  20. A method according to claim 1, wherein the induction medium is as shown in sequence list sequence numbers 1 to 15.

Description

Plant cultivation methods, uses, and products The present invention relates to the field of plant cultivation, specifically to a method of plant cultivation, an application, a target plant obtained, and a product. Artificial intervention methods for plant cultivation typically encompass various approaches, such as genetic engineering, cell engineering, and microbial engineering. To obtain new plant varieties, the aforementioned methods are generally used. New plant varieties introduced herein include whole plants, plant organs (e.g., flowers, fruits, seeds, roots, stems, leaves, etc.), plant tissues, plant cells, and other parts that may be derived from plants. Newly obtained plant varieties are typically expected to exhibit more optimized traits, such as optimized growth, increased yield, optimized biomass, enhanced structure, or optimized cell division. Increased yield can be achieved through various phenotypic factors, such as larger seed size or increased weight per seed/thousand seeds, an increase in the number of tillers in the root system, and other factors affecting plant yield, particularly increased yield of desired parts of the plant. The aforementioned expected effects are typically achieved through specific modification methods, such as altering traits by modifying plant DNA or histones. Even prior to the present invention, approaches regarding the demethylation of plant RNA for the purpose of optimizing plant traits have existed. Figure 1 schematically illustrates the demethylation of N6-methyladenine. Figure 2 shows the PCR-generated electrophoresis profile of a plant sample. FIG. 3 shows, as an example of embodiment 2, the delivery of a pCAMBIA1307 vector containing the FTO gene and the hygromycin resistance gene (Hygromycin) to Agrobacterium tumefaciens LBA4404. Figure 4 is an example of comparison between the whole plant and the rice grain between the early plant (Nipp) and the intermediate plant (FTO) using rice as Embodiment 2. Figure 5 is a figure comparing the entire plant and rice between the initial plant (Nipp) and the target plant (subsequent FTO) as an embodiment 2. Figure 6 is an example of comparison of the root systems of an early plant (Nipp), an intermediate plant (FTO), and a target plant (later FTO) using rice as Embodiment 2. To enable those skilled in the art to better understand the technical solution of the present invention, a more detailed description of the present invention is provided below, along with specific embodiments. Research for the present invention has revealed that specific enzymes can promote the methylation of RNA bases during plant growth and development. For example, the methyltransferase protein MTA can catalyze the N6-methylation of adenine bases within RNA, where "6-" refers to the sixth N in the forward or reverse direction from the N in the adenine base linked to the RNA sequence. After methylation, RNA can promote plant growth and development through subsequent processes, such as expression, or through products derived therefrom. Experimental evidence indicates that removing these methylation enzymes has adverse effects on plant growth and development; for example, plants may fail to produce viable seeds or seeds may not germinate normally. However, it has been further revealed that partially removing methyl groups from methylated RNA enhances the ability of the RNA or its products to promote plant growth and development, thereby facilitating trait optimization. This demethylation can be achieved using appropriate induction media. Typically, to obtain plants with optimized traits, it is common practice to select plants possessing an induction medium and maintain the optimized traits. However, the applicant of the present invention unexpectedly discovered that plants that have undergone RNA demethylation through a suitable induction medium can maintain the optimized traits even in the absence of the induction medium. Furthermore, these optimized traits can be stably inherited in some cases. In the study of this invention, it was discovered that by introducing a specific induction medium that promotes RNA demethylation into plants, the inherent characteristics of plants can be optimized, plants with optimized traits can be obtained even without the induction medium, and in particular, plant yield can be increased. This type of RNA demethylation induction medium primarily targets RNA that already contains methylated bases. Such induction media are mainly associated with demethylases such as FTO. The chemical mechanism of induction is illustrated in Figure 1, using the demethylation process of 6-methyladenine as an example. According to research results, although the specific RNA sequences demethylated by these induction media have not yet been clearly identified, it has been found that these induction media demethylate RNA containing methylated bases, thereby enabling the production of plants with optimized traits. A further advantage is that plan