KR-20260064448-A - Cosmetic composition for improving acne containing hemp callus extract as an effective ingredient

Abstract

The present invention provides a cosmetic composition for preventing and improving acne comprising a hemp callus extract as an active ingredient, which can rapidly treat acne due to high anti-inflammatory activity without side effects.

Inventors

• 김혁
• 임동우
• 유가람
• 김다훈

Assignees

• 토포랩 주식회사

Dates

Publication Date

20260507

Application Date

20250408

Priority Date

20241031

Claims (6)

1. A cosmetic composition for preventing and improving acne containing a callus extract derived from cannabis plant tissue as an active ingredient.
2. In paragraph 1, A cosmetic composition in which the above-mentioned callus derived from cannabis plant tissue is derived from branches, roots, leaves, stems, or flowers.
3. In paragraph 1, A cosmetic composition comprising the above extract in an amount of 0.1 to 10 parts by weight based on the total weight of the composition.
4. In paragraph 1, The above callus extract is a cosmetic composition prepared in the form of a wet paste or powder.
5. In paragraph 1, The above cosmetic composition is characterized by being in the form of a toner, skin toner, lotion, nourishing lotion, nourishing cream, massage cream, essence, pack, soap, liquid cleanser, gel, or patch.
6. A pharmaceutical composition for improving and treating acne comprising a callus extract derived from cannabis plant tissue as an active ingredient.

Description

Cosmetic composition for preventing and improving acne containing hemp callus extract as an effective ingredient The present invention relates to a cosmetic composition for preventing and improving acne, and more specifically, to a cosmetic composition for preventing and improving acne comprising hemp callus extract as an active ingredient. Plant callus refers to an undifferentiated, irregular mass of cells, also known as plant stem cells, which is the tissue undergoing vigorous division around a wound when a plant body is injured. Plants are broadly composed of meristematic tissue, which undergoes cell division, and non-meristematic tissue. When cells from the initial meristematic tissue are placed in a nutrient medium and grown, callus is formed, followed by the development of adventitious embryos, which eventually differentiate into plant bodies. Callus can be induced from various plant parts, such as stems, leaves, roots, or embryos. Callus culture is widely used in micropropagation—a technique that enables rapid plant reproduction in a controlled environment—making it efficient for the mass production of valuable plant species, including rare or endangered species. Furthermore, due to its vigorous division, it contains high concentrations of bioactive substances effective for anti-inflammation, antioxidant, anti-wrinkle, and whitening effects compared to ordinary plant tissues. In particular, because it contains various phytochemicals beneficial to human skin that differ from those found in fully differentiated plant tissues, it is utilized as a core material for cosmeceuticals and bio-cosmetics. Meanwhile, acne refers to a chronic inflammatory disease of the sebaceous glands characterized by the formation of comedones, papules, pustules, cysts, or nodules, which occurs mainly in adolescent boys and girls. It is a disease of the more than 3 million sebaceous glands in the skin and is an inflammatory disease that mainly occurs on the face, back, and chest. For the treatment of such acne, substances that regulate sebum, such as male hormones, tetracyclines, and benzoyl peroxide, are used; salicylic acid or retinoic acid preparations, which are vitamin A derivatives, are used to exfoliate pores; and topical antibiotics such as clindamycin, erythromycin, tetracycline, and sulfonamide are used. However, the use of male hormones and similar substances in cosmetics is regulated, and the therapeutic effects of salicylic acid preparations carry the risk of causing skin redness, edema, or skin aversion. Furthermore, the use of antibiotics may lead to resistance or hypersensitivity reactions, resulting in erythematous papules accompanied by itching. Therefore, there is a need to develop a treatment that effectively treats acne without side effects. In this regard, Korean Patent Publication No. 2024-0148991 discloses a composition for inhibiting inflammatory aging that includes liposomes derived from fermented hemp stem extract as an active ingredient. Figure 1 is a photograph showing the appearance of a callus induced from cannabis tissue in a culture medium according to one embodiment of the present invention. Figure 2 is a graph showing the results of analyzing the survival rate of macrophages treated with the hemp callus extract of the present invention. Figure 3 is a graph showing the results of analyzing the inhibitory effect of hemp callus extract treatment on NO (nitric oxide) production using mouse macrophage cell line RAW264.7. Figure 4a is a graph showing the results of analyzing the inhibitory effect of hemp callus extract treatment on the expression of the inflammatory cytokine TNF-α using ELISA. Figure 4b is a graph showing the results of analyzing the inhibitory effect of hemp callus extract treatment on the expression of the inflammatory cytokine interleukin-6 using ELISA. Figure 5a is a gel image showing the results of Western blot analysis of the inhibitory ability of the cannabis callus extract of the present invention on the expression of inflammatory protein markers. Figure 5b is a graph showing the results of a quantitative analysis of the expression level of Cox-2, an inflammatory protein marker. Figure 5c is a graph showing the results of a quantitative analysis of the expression level of iNOS, an inflammatory protein marker. Figure 5d is a graph showing the results of a quantitative analysis of the expression level of pERK, an inflammatory protein marker. Figure 6 is a graph showing the results of analyzing changes in skin soothing before and after use of a product containing the composition of the present invention. Figure 7 is a graph showing the results of analyzing the skin soothing improvement rate before and after using a product containing the composition of the present invention. Figure 8 is an image showing the degree of soothing following the use of the product on skin damaged by heat. Figure 9 is a graph showing the results of analyzing the change in skin temperature before and