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KR-20260064641-A - Manufacturing method of shell powder using mixed microbial culture

KR20260064641AKR 20260064641 AKR20260064641 AKR 20260064641AKR-20260064641-A

Abstract

The present invention relates to a method for manufacturing shell powder using complex microorganisms. Unlike conventional high-temperature calcination methods, the method for manufacturing shell powder using complex microorganisms according to the present invention does not require drying, high-temperature heat decomposition, and pulverization processes that take more than one year, allowing for immediate processing on-site. Furthermore, since it does not require the fuel-intensive high-temperature calcination process of over 900°C, it has the advantage of being able to decompose waste shells at an economical cost. In addition, the method for manufacturing shell powder according to the present invention is environmentally friendly as it enables the recycling of waste shells, which are aquatic waste, into shell fossil fertilizer.

Inventors

  • 방건웅
  • 홍한의
  • 서명희

Assignees

  • 방건웅
  • 홍한의
  • 서명희

Dates

Publication Date
20260507
Application Date
20251031
Priority Date
20241031

Claims (19)

  1. A complex microorganism deposited under accession number KCTC16521BP having conchiolin degrading ability and shell degrading ability.
  2. In paragraph 1, The above-mentioned complex microorganism is a thermophilic complex microorganism comprising 85% to 95% anaerobic microorganisms and 5% to 15% aerobic microorganisms, a complex microorganism deposited under accession number KCTC16521BP.
  3. In paragraph 1, The above-mentioned complex microorganism is a microbial community in which microorganisms of the genus Propionibacterium, the genus Prevotella, and the genus Lactobacillus are dominant species, a complex microorganism deposited under accession number KCTC16521BP.
  4. In paragraph 3, The above-mentioned complex microorganism is a complex microorganism deposited under accession number KCTC16521BP, which further comprises microorganisms of the genus Acetobacter, microorganisms of the genus Megasphaera, microorganisms of the genus Bacteroides, and microorganisms of the genus Komagataeibacter.
  5. A composition for producing shellfish powder comprising a complex microorganism deposited under accession number KCTC16521BP.
  6. In paragraph 5, A composition for producing shellfish powder, characterized in that the above-mentioned complex microorganisms decompose the calcium carbonate of shellfish to pulverize the shellfish.
  7. In paragraph 5, A composition for producing shellfish powder, wherein the above-mentioned complex microorganisms metabolize conchiolin as a substrate in a high-temperature environment.
  8. In paragraph 5, A composition for manufacturing shellfish powder, wherein the above-mentioned shellfish is one or more selected from the group consisting of oyster shells, scallop shells, abalone shells, cockle shells, clam shells, whelk shells, surf clam shells, and mussel shells.
  9. A step of preparing a thermophilic complex microorganism having microorganisms of the genus Propionibacterium, the genus Prevotella, and the genus Lactobacillus as dominant species; A step of adding the thermophilic complex microorganisms to shellfish and decomposing the shellfish at 70°C to 100°C, and A method for producing shellfish powder using complex microorganisms, comprising the step of obtaining shellfish powder.
  10. In Paragraph 9, The step of manufacturing the above thermophilic complex microorganism is A step of preparing mixed soil by collecting soil from limestone areas, volcanic rock areas, and waste shell landfills; A step of repeatedly culturing the above mixed soil in an organic medium for 25 to 35 days, alternating between aerobic and anaerobic conditions in a cycle of 10 to 30 minutes: 30 to 50 minutes (aerobic conditions: anaerobic conditions); and A method for producing shellfish powder using complex microorganisms, which further includes a step of isolating soil microbial communities.
  11. In Paragraph 10, A method for producing shellfish powder using complex microorganisms, characterized in that the above culture is a liquid culture.
  12. In Paragraph 9, A method for producing shellfish powder using a complex microorganism, wherein the above-mentioned thermophilic complex microorganism is a complex strain deposited under accession number KCTC16521BP.
  13. In Paragraph 9, The step of decomposing the above shellfish A method for producing shellfish powder using complex microorganisms, wherein 0.5 ml to 1.5 ml of complex microorganisms is added per 1 kg of shellfish.
  14. In Paragraph 13, The step of decomposing the above shellfish A method for producing shellfish powder using complex microorganisms, wherein 150 ml to 250 ml of water is further added per 1 kg of shellfish.
  15. In Paragraph 9, The step of decomposing the above shellfish A method for producing shellfish powder using complex microorganisms, characterized by being performed for 65 hours or more at a rotational speed of 2 rpm to 10 rpm.
  16. In Paragraph 9, A method for producing shellfish powder using complex microorganisms, wherein the step of decomposing the shellfish is performed in a fermentation tank.
  17. Shellfish powder produced by the manufacturing method of claim 9.
  18. In Paragraph 17, The above shellfish powder is a shellfish powder having a particle size of 0.05 μm to 1.5 μm.
  19. A shell fossil fertilizer composition comprising the shellfish powder of claim 17.

Description

Manufacturing method of shell powder using mixed microbial culture The present invention relates to a method for manufacturing seashell powder using complex microorganisms. Recently, the types of waste generated during industrialization and daily life are very diverse, and environmental pollution caused by these wastes is becoming increasingly serious day by day. As the harmful effects become a reality, various waste treatment technologies, pollution prevention technologies, and policies are being proposed to address this. Among the shells generated domestically, the biggest headache is oyster shells. Oysters account for the largest share of the domestic aquaculture industry as a single species. Annual production averages over 300,000 tons, making it the second-largest volume globally after China's 540,000 tons.1) The problem lies with the oyster shells generated after harvesting; while about 70% of these are recycled for purposes such as spat, over 60,000 tons of shells remain, causing serious environmental issues such as foul odors and water pollution. Therefore, the government is currently guiding policies toward recycling industrial and household waste whenever possible, and accordingly, many researchers are conducting extensive research on the recycling of seashells. As mentioned above, most seashells are disposed of through landfilling, but regarding methods for recycling some seashells, the technology described in Korean Registered Patent 10-0430815 (Method for manufacturing organic fertilizer using an alkali stabilization method for seashells) and Registered Patent 10-0784495 (Method for manufacturing inorganic thermal insulation material using seashells) and the method for recycling seashells into seashell fertilizer by crushing them is described. Additionally, the technology described in Registered Utility Model 20-0190143, Registered Patent 10-0806396 (Building material using seashells and method of manufacturing the same), and Registered Patent 20-0165053 (Building material recycled from loess and seashells) describes a method for recycling seashells into building materials. These technologies share the common feature of pulverizing waste seashells by calcining them at high temperatures. The background description of the invention is provided to facilitate a better understanding of the present invention. The matters described in the background description should not be construed as an acknowledgment that they exist as prior art. FIG. 1a illustrates an exemplary method for manufacturing shellfish powder according to one embodiment of the present invention. FIG. 1b illustrates an exemplary method for producing complex microorganisms according to one embodiment of the present invention. Figure 2 illustrates the results of microbial identification of a complex microorganism according to one embodiment of the present invention. Figure 3 illustrates an example of preparing shellfish powder using complex microorganisms according to one embodiment of the present invention. Figure 4 shows the scanning electron microscope observation results of shellfish powder according to one embodiment of the present invention. Figure 5 shows the results of particle size analysis using a particle size analyzer of shellfish powder according to one embodiment of the present invention. Figure 6 illustrates the results of the crystal structure analysis of shellfish powder according to one embodiment of the present invention. The advantages and features of the present invention and the methods for achieving them will become clear by referring to the embodiments described below in detail together with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below but may be implemented in various different forms. These embodiments are provided merely to ensure that the disclosure of the present invention is complete and to fully inform those skilled in the art of the scope of the invention, and the present invention is defined only by the scope of the claims. In the following, terms used within this specification are explained for clarity of explanation. In this document, expressions such as "have," "can have," "include," or "can include" refer to the existence of the relevant feature (e.g., numerical values, functions, actions, or components, etc.) and do not exclude the existence of additional features. In this document, "or" means "and/or" unless otherwise noted. Expressions such as "A or B," "at least one of A or/and B," or "one or more of A or/and B" may include all possible combinations of items listed together. For example, "A or B," "at least one of A and B," or "at least one of A or B" may refer to cases including (1) at least one A, (2) at least one B, or (3) both at least one A and at least one B. In this document, '%' used to indicate the concentration of a specific substance is (w/w) % for solid/solid, (w/v) % for solid/liquid, and (v/v) % for liquid/liquid, unless ot