KR-20260064811-A - Compositions for controlling bacterial or fungal diseases in plants by antimicrobiological and resistance-inducing activities of Pediococcus inopinatus M21F004 strain, manufacturing method thereof, and method for controlling phytopathogenic bacteria or fungi

Abstract

One aspect relates to a composition for controlling plant diseases comprising the Pediococcus inopinatus M21F004 strain, a culture solution thereof, and an active substance. The Pediococcus inopinatus M21F004 strain of one aspect has antibacterial activity against various plant pathogenic fungi or bacteria and contains oleic acid, which has excellent antifungal effects, as an active substance, so it can be usefully used for controlling plant diseases.

Inventors

• 권용민
• 정다운
• 황현주
• 최그레이스
• 이대성
• 박애란
• 유난희
• 최은아

Assignees

• 국립해양생물자원관
• 주식회사 잰153바이오텍

Dates

Publication Date

20260508

Application Date

20241029

Claims (12)

1. Pediococcus inopinatus strain M21F004 deposited under accession number KCTC 15428BP.
2. The strain of claim 1, wherein the strain comprises the 16S rRNA of SEQ ID NO. 3.
3. In claim 1, the strain is (i) producing one or more of oleic acid, 3-isobutylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione, palmitic acid, and 3-benzyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione (ii) A strain having activity to control plant diseases caused by fungi or bacteria.
4. A composition for controlling plant diseases caused by fungi or bacteria, comprising one or more selected from the group consisting of the strain of claim 1, its dried product, crushed product, lysate, culture solution, culture, extracts thereof, and fractions of the extract; or oleic acid.
5. A composition for controlling plant diseases caused by fungi or bacteria according to claim 4, wherein the strain, dried material, crushed material, lysate, culture solution, culture, extract, or fraction comprises one or more of oleic acid, 3-isobutylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione, palmitic acid, and 3-benzyl-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione.
6. In claim 4, the composition for controlling plant diseases caused by fungi or bacteria inhibits the growth of fungi or bacteria or induces resistance of plants to fungi or bacteria.
7. A composition for controlling plant diseases caused by fungi or bacteria according to claim 4, wherein the plant disease is apple crown gall, stone fruit bacterial spot, tomato bacterial wilt, cabbage soft rot, turf coin blight, rice sheath blight, cucumber vine splitting disease, or damping-off.
8. A composition for controlling plant diseases caused by fungi or bacteria according to claim 4, wherein the fungus is one or more of Clarireedia homoeocarpa , Rhizoctonia solani , Fusarium oxysporum f. sp. cucumerinum , and Pythium aphanidermatum .
9. A composition for controlling plant diseases caused by fungi or bacteria according to claim 4, wherein the bacteria are one or more of Agrobacterium tumefaciens , Xanthomonas arboricola pv. pruni , Ralstonia solanacearum , and Pectobacterium carotovorum subsp. Carotovorum .
10. A method for preparing a composition for controlling plant diseases caused by fungi or bacteria according to any one of claims 4 to 9, comprising the step of culturing the Pediococcus inopinatus M21F004 strain deposited under accession number KCTC 15428BP.
11. A method for controlling plant diseases caused by fungi or bacteria, comprising the step of treating a plant, its seeds, or its habitat with a composition for controlling plant diseases caused by fungi or bacteria according to any one of claims 4 to 9.
12. A method for controlling plant diseases caused by fungi or bacteria according to claim 11, wherein the treatment step is performed in one or more ways selected from the group consisting of spraying, soil drenching, surface spraying, rhizosphere treatment, seed treatment, immersion, poisoning, and fumigation.

Description

Compositions for controlling bacterial or fungal diseases in plants by antimicrobiological and resistance-inducing activities of Pediococcus inopinatus M21F004 strain, manufacturing method thereof, and method for controlling phytopathogenic bacteria or fungi The present invention relates to a novel Pediococcus inopinatus M21F004 strain, a composition for controlling bacterial or fungal diseases in plants through the antimicrobial activity and induced resistance thereof, a method for manufacturing the same, and a method for controlling plant pathogenic bacteria or fungi. The present invention was made with the support of the National Marine Biological Resources Institute, with the research project title being "Identification of Plant Disease Control Characteristics of Marine Lactic Acid Bacteria and Research on Culture Process," the research institution being Gen153 Biotech Co., Ltd., and the research period being January 1, 2023 – December 31, 2023. Plant diseases caused by pathogenic bacteria and fungi have a direct or indirect impact, significantly reducing agricultural yields. Consequently, chemical antibiotics and fungicides are used worldwide to manage plant diseases in various crops. However, the misuse and overuse of synthetic pesticides, which constitute the majority of pesticides, have raised various issues, including soil, water, and agricultural product contamination, toxicity, ecosystem disruption, and the emergence of resistant strains. In response, OECD countries have recently been aiming to reduce the use of synthetic pesticides by more than 40%. Furthermore, with the increasing interest in well-being and the surging demand for organic produce in developed nations, there is an urgent need to develop eco-friendly biological agents capable of controlling plant diseases. Recently, microbial pesticides have been researched as an alternative to conventional chemical treatments for controlling plant diseases. It has been confirmed that various bacteria and fungi possess excellent antimicrobial activity for controlling plant diseases, demonstrating their potential as effective microbial pesticides. Most developed microbial pesticides are derived from bacteria, accounting for approximately 90%, while fungal-derived microbial agents account for 10%. Types of biological control against plant pathogens include direct antagonism, direct-indirect antagonism, and indirect antagonism. Direct antagonism involves mechanisms of overparasitism and predation by various fungi and bacteria, while direct-indirect antagonism includes control through antibiotics and degrading enzymes. Indirect antagonism includes competitive and induced resistance mechanisms. In particular, induced resistance is a phenomenon in which a host plant's resistance to pathogen invasion is induced by specific biological or non-biological factors; it enhances the plant's defense capabilities against microbial invasion or similar stresses and is characterized by its long-term persistence once expressed. Recently, interest in induced resistance mechanisms has been increasing in terms of improving agricultural ecosystems and biological control of various plant diseases. Marine microorganisms must survive under harsh conditions such as high pressure, anaerobic conditions, low temperatures, high acidity, and high ambient temperatures, and must also adapt to conditions involving high salinity, radiation, light, and minimal nutrients. Consequently, extreme conditions in the marine environment lead to genetic and metabolic diversity among marine microorganisms, which ultimately stimulates special adaptation mechanisms, particularly the synthesis of rare active compounds. Among these, lactic acid bacteria, which are beneficial marine microorganisms, are attracting significant attention due to their ability to produce various active compounds, including antimicrobial compounds such as various bacteriological enzymes, organic acids, and bacteriocins. Lactic acid bacteria can be rod-shaped or spherical and constitute a diverse group of Gram-positive, non-motile, acid-resistant microorganisms. They are characterized by not forming spores, lacking catalase activity, and being able to proliferate even in low pH environments. Generally, lactic acid bacteria require small amounts of oxygen and possess strong adaptability to extreme anaerobic and acidic conditions. They are used as acidulants, flavor enhancers, and preservatives, and also produce the biodegradable polymer polylactic acid as a monomer. Representative genera of lactic acid bacteria include Lactobacillus, Leuconostoc, Pediococcus, Lactococcus, Enterococcus, and Streptococcus, and active research is currently underway regarding their potential application as microbial pesticides. Due to these characteristics, the genus Pediococcus , a type of lactic acid bacteria, holds excellent potential as an antimicrobial microbial control agent for the control of plant pathogens. Accordingly, the inventors o